Survey on forensic DNA biology training in forensic science service laboratories in the United States.

Training is an essential component of onboarding new hires in forensic science service provider (FSSP) laboratories. There are several DNA training standards published by the American Academy of Forensic Sciences (AAFS) Academy Standards Board (ASB) American National Standards Institute (ANSI) accredited framework. In this study, we conducted a survey of forensic DNA laboratory training programs to better understand training activities and materials.

Development of Polymerase Chain Reaction-High-Resolution Melt Assay for Waterborne Pathogens , , and .

is the waterborne pathogen primarily responsible for causing both Pontiac Fever and Legionnaire's Disease in humans. is transmitted via aerosolized water droplets. The purpose of this study was to design and test primers to allow for rapid polymerase chain reaction (PCR) melt detection and identification of this infectious agent in cases of clinical or emergency response detection.

Live Vaccine Strain training of murine alveolar and bone marrow-derived macrophages.

Unlabelled: Traditionally, successful vaccines rely on specific adaptive immunity by activating lymphocytes with an attenuated pathogen, or pathogen subunit, to elicit heightened responses upon subsequent exposures. However, recent work with and other pathogens has identified a role for "trained" monocytes in protection through memory-like but non-specific immunity. Here, we used an co-culture approach to study the potential role of trained macrophages, including lung alveolar macrophages, in immune responses to the Live Vaccine Strain (LVS) of is an intracellular bacterium that replicates within mammalian macrophages and causes respiratory as well as systemic disease.

Systems genetics uncover new loci containing functional gene candidates in Mycobacterium tuberculosis-infected Diversity Outbred mice.

Mycobacterium tuberculosis infects two billion people across the globe, and results in 8-9 million new tuberculosis (TB) cases and 1-1.5 million deaths each year. Most patients have no known genetic basis that predisposes them to disease.

Host Genetic Background Influences BCG-Induced Antibodies Cross-Reactive to SARS-CoV-2 Spike Protein.

Bacillus Calmette-Guérin (BCG) protects against childhood tuberculosis; and unlike most vaccines, BCG broadly impacts immunity to other pathogens and even some cancers. Early in the COVID-19 pandemic, epidemiological studies identified a protective association between BCG vaccination and outcomes of SARS-CoV-2, but the associations in later studies were inconsistent. We sought possible reasons and noticed the study populations often lived in the same country.