When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.

In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can block the progression of transcribing RNA polymerase (RNAP), leading to RNAP pausing and ultimately dissociation from the DNA template. In this review, we discuss the mechanisms by which transcriptional roadblocks can impede RNAP progression, as well as how RNAP can overcome these obstacles to continue transcription. We examine different DNA-binding proteins involved in transcriptional roadblocking and their biophysical properties that determine their effectiveness in blocking RNAP progression.

Efficient -demethylation of lignin monoaromatics using the peroxygenase activity of cytochrome P450 enzymes.

A crucial reaction in harnessing renewable carbon from lignin is -demethylation. We demonstrate the selective -demethylation of syringol and guaiacol using different cytochrome P450 enzymes. These can efficiently use hydrogen peroxide which, when compared to nicotinamide cofactor-dependent monooxygenases and synthetic methods, allows for cheap and clean -demethylation of lignin-derived aromatics.

RNA polymerase efficiently transcribes through DNA-scaffolded, cooperative bacteriophage repressor complexes.

DNA can act as a scaffold for the cooperative binding of protein oligomers. For example, the phage 186 CI repressor forms a wheel of seven dimers wrapped in DNA with specific binding sites, while phage λ CI repressor dimers bind to two well-separated sets of operators, forming a DNA loop. Atomic force microscopy was used to measure transcription elongation by Escherichia coli RNA polymerase (RNAP) through these protein complexes.

APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting and MRSA Biofilms.

The high infection and mortality rate of methicillin-resistant Staphylococcus aureus (MRSA) necessitates the urgent development of new treatment strategies. Bacteriophages (phages) have several advantages compared to antibiotics for the treatment of multi-drug-resistant bacterial infections, and thus provide a promising alternative to antibiotics. Here, S.

Editors' Roundup: April 2022.

This Commentary represents the first instalment of a regular feature that seeks to fulfil one of Biophysical Reviews' IUPAB mandated goals-that of assisting in the international promotion of biophysical research. Known as the 'Editors' Roundup', this Commentary feature is a multi-author collective description of recently published research from journals publishing material across the biophysical realm. Although Biophysical Reviews is published by Springer-Nature, the source of contributed articles is unrestricted and can include different commercial and society publishers.