Evaluation of lymphocytes' influence in luminol-dependent chemiluminescence emission of mouse spleen cells.

The focus of this study was to elucidate the influence of lymphocytes on phagocytic cell-dependent chemiluminescence (CL). CL was induced by in vitro addition of mitogens, particulate substances and Keyhole Limpet Hemocyanin (KLH)-antigen. CL was measured in spleen cells of immunologically naive mice and in mice immunized with KLH.

Chemiluminescence response of peritoneal exudate cells from mice infected with virulent and avirulent strains of Toxoplasma gondii.

The zymosan-triggered chemiluminescence response of peritoneal exudate cells from mice infected with relatively virulent or avirulent Toxoplasma gondii strains was examined at various intervals after infection. Toxoplasma infections increased the background chemiluminescence compared with controls, but higher values were attained earlier in virulent than avirulent infection. A phase of transient unresponsiveness to zymosan was observed with both types of toxoplasma strains.

Influence of dextran polymer particles (Sephadex G-200) on the chemiluminescence response of murine peritoneal leukocytes.

Murine peritoneal leukocytes exhibited enhanced levels of chemiluminescence (Cl) when derived from mice inoculated intraperitoneally with Sephadex G-200 gel. This could be observed in the background, 'spontaneous' Cl reactivity, and in Cl elicited by in vitro addition of zymosan or mitogens. Leukocytes found in the peritoneum after Sephadex G-200 inoculation are predominantly neutrophils.

Bacteria and zymosan opsonized with histone, dextran sulfate, and polyanetholesulfonate trigger intense chemiluminescence in human blood leukocytes and platelets and in mouse macrophages: modulation by metabolic inhibitors in relation to leukocyte-bacteria interactions in inflammatory sites.

Human blood leukocytes and platelets and mouse peritoneal macrophages emit very rapid and very intense Luminol-dependent chemiluminescence (CL) signals when treated with streptococci, staphylococci, or with zymosan, which have been preopsonized with arginine-rich histone, dextran sulfate or polyanetholesulfonate (liquoid). Liquoid alone at 10-30 micrograms/2 X 10(5) leukocytes also triggers intense CL responses in the absence of a carrier. Strong CL can also be triggered, and at the same levels, when the various polyelectrolytes are simply mixed with the bacteria or zymosan and added to the leukocyte suspensions.

Influence of dextran polymer particles (Sephadex G-200) on humoral immune responses.

Dextran polymer particles (Sephadex G-200) mixed with bovine serum albumin (BSA) or sheep red blood cells (SRBC) were injected intraperitoneally (i.p.) into mice and the number of antibody-forming cells in spleen and antibody titers in sera were determined.