Publications by authors named "K E Fladmark"
Accelerated Sarcopenia Phenotype in the DJ-1/-Knockout Zebrafish.
Antioxidants (Basel)
December 2024
Age-dependent loss of muscle mass and function is associated with oxidative stress. DJ-1/ acts as an antioxidant through multiple signalling pathways. DJ-1-knockout zebrafish show a decline in swimming performance and loss of weight gain between 6 and 9 months of age.
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- Graphene is a special material made of carbon atoms and could be useful in medicine, but we need to check how safe it is for living things.
- We compared two ways to make graphene: one method (sonication) gave us more toxic graphene, while the other method (microfluidization) produced safer graphene.
- In tests with blood and zebrafish larvae, we found that sonicated graphene was much more harmful than microfluidized graphene, which was less toxic and caused fewer changes in immune cell behavior.
Parkinson's disease is characterized neuropathologically by the degeneration of dopaminergic neurons in the ventral midbrain, the accumulation of α-synuclein (α-syn) aggregates in neurons and chronic neuroinflammation. In the past two decades, in vitro, ex vivo and in vivo studies have consistently shown the involvement of inflammatory responses mediated by microglia and astrocytes, which may be elicited by pathological α-syn or signals from affected neurons and other cell types, and are directly linked to neurodegeneration and disease development. Apart from the prominent immune alterations seen in the CNS, including the infiltration of T cells into the brain, more recent studies have demonstrated important changes in the peripheral immune profile within both the innate and adaptive compartments, particularly involving monocytes, CD4+ and CD8+ T cells.
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- Parkinson's disease (PD) is a chronic, progressive condition linked to neuroinflammation and immune dysfunction, requiring a better understanding of immune system roles in its development.
- Different methodological approaches are used to study immune changes in PD, each with advantages and limitations, highlighting the need for both animal and in vivo models to validate findings.
- There is potential for discovering therapeutic targets by exploring immune-mediated mechanisms in PD, emphasizing the necessity for established guidelines in experimental research for accuracy and reliability.
DJ-1 is a redox sensitive protein with a wide range of functions related to oxidative stress protection. Mutations in the gene, which codes for DJ-1 are associated with early onset familial Parkinson's disease and increased astrocytic DJ-1 levels are found in pathologic tissues from idiopathic Parkinson's disease. We have previously established a DJ-1 knockout zebrafish line that developed normally, but with aging the DJ-1 null fish had a lowered level of tyrosine hydroxylase, respiratory mitochondrial failure and a lower body mass.
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