Publications by authors named "K Chousalkar"

This study evaluated the impact of vaccine diluents (peptone or water) on the protective effects of Typhimurium (. Typhimurium) vaccine. Vaccinated broilers were challenged with different doses of wild-type .

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Salmonella contamination of eggs is a global food safety concern, producers, regulatory authorities, and affecting public health. To mitigate Salmonella risks on-farm and along the supply chain, egg producers have adopted various quality assurance, animal husbandry, and biosecurity practices recommended by organizations such as Australian eggs, the European Commission, and the US Department of Agriculture (USDA). However, egg storage requirements vary significantly worldwide.

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Article Synopsis
  • - Salmonella, a common foodborne pathogen, is often found in raw eggs, and safety guidelines suggest using food acids to reduce its presence; however, some strains can become tolerant to acidic environments.
  • - This study explored the persistence of acid-tolerant Salmonella strains in mayonnaise versus sensitive strains, using various methods to generate and assess these strains.
  • - Results showed that acid-tolerant strains survived longer and formed more biofilms in mayonnaise, indicating that current food safety measures may not be sufficient to eliminate Salmonella effectively.
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One of the commonly used methods to control on-farm outbreaks in the poultry industry is vaccination. Vaccine diluents may affect vaccine efficacy but there are limited studies on how different diluents affect the colonization of live, attenuated vaccines. In this study, buffered peptone water, Marek's diluent and water were used as the diluents to reconstitute the commercially available Typhimurium vaccine (Vaxsafe® ST).

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Role of dust in Salmonella transmission on chicken farms is not well characterised. Salmonella Typhimurium (ST) infection of commercial layer chickens was investigated using a novel sprinkling method of chicken dust spiked with ST and the uptake compared to a conventional oral infection. While both inoculation methods resulted in colonisation of the intestines, the Salmonella load in liver samples was significantly higher at 7 dpi after exposing chicks to sprinkled dust compared to the oral infection group.

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