Nucleotide-resolution Mapping of RNA N6-Methyladenosine (m6A) modifications and comprehensive analysis of global polyadenylation events in mRNA 3' end processing in malaria pathogen .

is an obligate human parasite of the phylum Apicomplexa and is the causative agent of the most lethal form of human malaria. Although N6-methyladenosine modification is thought to be one of the major post-transcriptional regulatory mechanisms for stage-specific gene expression in apicomplexan parasites, the precise base position of m6A in mRNAs or noncoding RNAs in these parasites remains unknown. Here, we report global nucleotide-resolution mapping of m6A residues in using DART-seq technology, which quantitatively displayed a stage-specific, dynamic distribution pattern with enrichment near mRNA 3' ends.

A highly optimized and sensitive bowtie shape-based SPR biosensor for different analyte detection.

With advancements in photonic technologies, photonic crystal fibers (PCFs) have become crucial components in developing highly sensitive and efficient biosensors. This paper presents an optimized bowtie-shaped PCF biosensor that leverages surface plasmon resonance (SPR) phenomena for enhanced refractive index (RI) sensing. The proposed design uses an external sensing mechanism to effectively characterize performance across an RI range of 1.

Anatomic and Visual Outcomes of Scleral Buckle in Primary Rhegmatogenous Retinal Detachment in the Era of Pars Plana Vitrectomy.

To determine the presenting clinical characteristics of patients with rhegmatogenous retinal detachment (RRD) who underwent scleral buckling and the impact on final visual outcomes and to determine the postoperative primary attachment rate, complications, and subsequent management. This retrospective observational study comprised patients with RRD who had scleral buckling surgery from January 2016 to December 2021 with a minimum follow-up of 6 months. The study comprised 307 eyes of 290 patients; 97% of eyes were phakic.

A pilot trial of integrating the Patient-Reported Outcome Measurement Information System (PROMIS®) into rheumatology care.

Objectives: Utilising Patient-Reported Outcomes Measurement Information System (PROMIS®) questionnaires can enhance clinical care by measuring longitudinal changes in symptom severity as reported by the patient. The aim of this pilot study was to assess the feasibility and impact of incorporating PROMIS® questionnaires at the point-of-care in rheumatology practice.

Methods: Patients with rheumatic diseases and decrements in ≥1 PROMIS® domain (pain intensity, physical function, or sleep disturbance) were stratified by their concerning domain, then randomised to either receive an interpretation of their PROMIS® scores prior to their rheumatology appointment (Arm 1) or to usual care (Arm 2) (ClinicalTrials.

Theoretical study of a new molecular ion species BeN: molecular states and cross sections for electronic excitation and dissociation.

We present here a theoretical study of a new molecular ion species BeN, which has not been reported before. Using a good quality configuration interaction model, we first undertake a configuration interaction calculation of the molecular states to obtain the potential energy curves of several low lying states of BeN and the dipole and transition dipole moments of some of its states as a function of the internuclear distance. We find the ground state to be bound and of X Σ character having a shallow minimum at = 2.