Exploring Excited-State Intramolecular Proton-Coupled Electron Transfer in Dinuclear Ir(III)-Complex via Covalently Tagged Hydroquinone: Phototherapy Through Futile Redox Cycling.

Anticipating intramolecular excited-state proton-coupled electron transfer (PCET) process within dinuclear Ir-photocatalytic system via the covalent linkage is seminal, yet challenging. Indeed, the development of various dinuclear complexes is also promising for studying integral photophysics and facilitating applications in catalysis or biology. Herein, this study reports dinuclear [Ir(bis{imidazo-phenanthrolin-2-yl}-hydroquinone)(ppy)] (1) complex by leveraging both ligand-centered redox property and intramolecular H-bonding for exploring dual excited-state proton-transfer assisted PCET process.

A Viewpoint on the Ethics of Pseudostuttering Assignments: Guidelines and Best Practices for Their Use.

Purpose: Pseudostuttering, or the act of voluntarily stuttering or stuttering on purpose, has been both regularly used by clinicians alongside clients in stuttering therapy and taught to students in stuttering courses for decades. However, in recent years, teaching speech-language pathology students how to pseudostutter in stuttering courses has been increasingly questioned by students on grounds that pseudostuttering may be ableist, a disability simulation, and of questionable clinical value. The purpose of this article is to discuss the value and ethics of pseudostuttering assignments as part of graduate clinical education for speech-language pathologists (SLPs).

Synthesis of N-Methyluridine- and 2'-O-Alkyl/2'-Fluoro-N-Methyluridine-Modified Phosphoramidites and Their Incorporation into DNA and RNA Oligonucleotides.

In this article, we describe the synthesis of N-methyluridine (mU) and 2'-O-alkyl/2'-fluoro-N-methyluridine (2'-O-alkyl/2'-F-mU) phosphoramidites as well as their incorporation into a 14-mer DNA and RNA oligonucleotide sequence. Synthesis of the 2'-O-alkyl-mU phosphoramidite starts with commercially available uridine to achieve a tritylated mU intermediate, followed by 2'-O-alkylation and finally phosphitylation. Synthesis of the 2'-F-mU phosphoramidite is obtained from a commercially available 2'-F-uridine nucleoside.

Selective Recognition of the Dimeric NG16 Parallel G-Quadruplex Structure Using Synthetic Turn-On Red Fluorescent Protein Chromophore.

Red fluorescent protein (RFP)-based fluorescent probes that can selectively interact with specific nucleic acids are of great importance for therapeutic and bioimaging applications. Herein, we have reported the synthesis of RFP chromophores for selective recognition of G-quadruplex nucleic acids in vitro and ex vivo. We identified as a fluorescent turn-on probe that binds to the dimeric NG16 parallel quadruplex with superior selectivity and sensitivity over various parallel, antiparallel, and hybrid topologies.

Investigating the Effect of Chemical Modifications on the Ribose Sugar Conformation, Watson-Crick Base Pairing, and Intrastrand Stacking Interactions: A Theoretical Approach.

Over the last few decades, chemically modified sugars have been incorporated into nucleic acid-based therapeutics to improve their pharmacological potential. Chemical modification can influence the sugar conformation, Watson-Crick hydrogen (W-C) bonding, and nucleobase stacking interactions, which play major roles in the structural integrity and dynamic properties of nucleic acid duplexes. In this study, we categorized 33 uridine (U*) and cytidine (C*) sugar modifications and calculated their sugar conformational parameters.