Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro.

Drug discovery is associated with high levels of compound elimination in all stages of development. The current practices for the pharmacokinetic testing of intestinal absorption combine Transwell inserts with the Caco-2 cell line and are associated with a wide range of limitations. The improvement of pharmacokinetic research relies on the development of more advanced in vitro intestinal constructs that better represent human native tissue and its response to drugs, providing greater predictive accuracy.

Factor model for ordinal categorical data with latent factors explained by auxiliary variables applied to the major depression inventory.

In behavioral and social research, questionnaires are an important assessment tool, through which individuals can be categorized according to how they classify themselves in respect to a personal trait. One example is the Major Depression Inventory (MDI), which is widely used for the assessment of depression. It can also be used as a depression severity scale, with scores ranging from 0 to 50 constructed considering the same weight for each item in the MDI.

Investigation into the significant role of dermal-epidermal interactions in skin ageing utilising a bioengineered skin construct.

Increased prevalence of skin ageing is a growing concern due to an ageing global population and has both sociological and psychological implications. The use of more clinically predictive in vitro methods for dermatological research is becoming commonplace due to initiatives and the cost of clinical testing. In this study, we utilise a well-defined and characterised bioengineered skin construct as a tool to investigate the cellular and molecular dynamics involved in skin ageing from a dermal perspective.