Breaking the Cycle of Human Trafficking.

Resources to help nurses identify, assess, and care for victims.

Structural characteristics of the plasmid-encoded toxin from enteroaggregative Escherichia coli.

Intoxication by the plasmid-encoded toxin (Pet) of enteroaggregative Escherichia coli requires toxin translocation from the endoplasmic reticulum (ER) to the cytosol. This event involves the quality control system of ER-associated degradation (ERAD), but the molecular details of the process are poorly characterized. For many structurally distinct AB-type toxins, ERAD-mediated translocation is triggered by the spontaneous unfolding of a thermally unstable A chain.

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway.

The plasmid-encoded toxin (Pet) of enteroaggregative Escherichia coli is a 104-kDa autotransporter protein that exhibits proteolytic activity against the actin-binding protein alpha-fodrin. Intracellular cleavage of epithelial fodrin by Pet disrupts the actin cytoskeleton, causing both cytotoxic and enterotoxic effects. Intoxication requires the serine protease activity of Pet and toxin endocytosis from clathrin-coated pits.

Stability over age and sex of children's responses to embedded figures test.

240 children in Grades K to 4 were administered the entire Children's Embedded Figures Test to determine whether similar factor structures are obtained from samples of different sex and age as well as when the population is randomly split. Data were analyzed by principal axis factor analysis and relevant factor structures were compared using Veldman's 1967 program "Relate". There was one stable factor which was consistent across sex and accounted for about 19% of the variance.

Factor structure of shortened Children's Embedded Figures Test for different age groups.

240 children, 24 of each sex in Grades K to 4, were administered the entire Children's Embedded Figures Test, regardless of the failure rule. Factor loadings for items from a shortened version of the test were examined for a randomly divided sample, a sample divided by sex, a sample divided in two grade groupings, and an undivided total population. Stable factors were found for the total sample and when the sample was divided by sex.