Publications by authors named "K BUJKO"
Populations of very small embryonic-like stem cells (VSELs) (CD34+lin-CD45- and CD133+lin-CD45-), circulating in the peripheral blood of adults in small numbers, have been identified in several human tissues and together with the populations of hematopoietic stem cells (HSCs) (CD34+lin-CD45+) and CD133+lin-CD45+constitute a pool of cells with self-renewal and pluripotent stem cell characteristics. Using advanced cell staining and sorting strategies, we isolated populations of VSELs and HSCs for bulk RNA-Seq analysis to compare the transcriptomic profiles of both cell populations. Libraries were prepared from an extremely small number of cells; however, their good quality was preserved, and they met the criteria for sequencing.
View Article and Find Full Text PDFBackground: Guidelines recommend elective irradiation of the external iliac and upper pelvic lymph nodes (LNs) regardless of clinical stage, but the supporting evidence for this recommendation is limited.
Methods: We conducted a retrospective analysis of 68 consecutive patients with squamous cell carcinoma of the anal canal who underwent volumetric modulated arc therapy chemoradiation, excluding external iliac LNs from elective irradiation. In patients with negative bilateral inguinal LNs, both external iliac regions were omitted, while in those with unilateral positive inguinal LNs, only the ipsilateral external iliac region was included and the contralateral side was omitted.
Article Synopsis
- Researchers identified a specific population of Very Small Embryonic-Like Stem Cells (VSELs) in human postnatal tissues such as bone marrow, peripheral blood, and umbilical cord blood that can differentiate into various tissue stem cells.
- Molecular analysis showed that these VSELs share similar characteristics with embryonic stem cells, including certain gene expressions and patterns of genetic imprinting.
- Using single-cell RNA sequencing, the study found that these human VSELs also exhibit subpopulations related to germline development and are influenced by factors that control differentiation and immune response.