Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET.

Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated "in-line reader" for high-throughput drug discovery.

Employing new grads: a plan for success.

The authors describe an elegant goal-driven program designed to prepare new nursing graduates to function optimally on a hospital unit by focusing first on their security and affiliative needs [belonging], and subsequently on their professional skill and knowledge acquisition. This developmental program has both a clear timeline and structure as well as well defined role expectations for the experienced RN guide/mentor, the orientee, and the unit's nurse manager and staff. The guide/mentors are carefully selected and prepared for their new roles as well.

Cow-calf association delays postpartum ovulation in mastectomized cows.

Eleven 3-yr-old crossbred Angus cows that had raised one calf each were mastectomized at 6 to 7 mo of their second gestation. All calves were removed from cows within 12 h after birth. Cows were kept in a drylot with a fertile bull for 60 d after parturition.

Factors associated with shortened estrous cycles after abortion in beef heifers.

Forty-six crossbred Hereford heifers were allotted into five experimental groups. Fenprotalene, a prostaglandin analogue, was administered to induce abortion and (or) to regress the corpus luteum (CL) in four groups of heifers about 75 d after conception. The four groups were 1) pregnant heifers, 2) pregnant heifers with the ovary contralateral to the gravid uterine horn removed 24 h after fenprostalene injection, 3) same as Group 2 but with the ipsilateral, rather than the contralateral, ovary removed and 4) heifers with uterus removed (ovaries intact) about 35 d after conception.