Genome-scale evolution in local populations of wild chimpanzees.

Analysis of genome-scale evolution has been difficult in large, endangered animals because opportunities to collect high-quality genetic samples are limited. There is a need for novel field-friendly, cost-effective genetic techniques. This study conducted an exome-wide analysis of a total of 42 chimpanzees (Pan troglodytes) across six African regions, providing insights into population discrimination techniques.

mutant newts regenerate normal hindlimbs despite severe developmental defects.

In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function is broadly conserved in tetrapods and/or involved in adult limb regeneration remains unknown. To tackle this question, we established mutant lines in the newt which has amazing regenerative ability. While mutant forelimbs develop normally, the hindlimbs fail to develop and downregulate FGF target genes.

Physical activity of children and adolescents with disabilities in Poland - First Para Report Card.

Background: This study provides a comprehensive evaluation of the Polish Para Report Card, which assesses various aspects of physical activity (PA) and related indicators among Polish children and adolescents with disabilities. This area has been under-researched, especially in the Polish context.

Methods: The research methodology included systematic literature searches from 2014 to 2022, utilizing databases such as PubMed, EBSCO, and Google Scholar.

Isolation of planarian viable cells using fluorescence-activated cell sorting for advancing single-cell transcriptome analysis.

Preparing viable single cells is critical for conducting single-cell RNA sequencing (scRNA-seq) because the presence of ambient RNA from dead or damaged cells can interfere with data analysis. Here, we developed a method for isolating viable single cells from adult planarian bodies using fluorescence-activated cell sorting (FACS). This method was then applied to both adult pluripotent stem cells (aPSCs) and differentiating/differentiated cells.