[Spheroid formation in explants of human breast tumor and fibroadenomatous tissues and lymphoid infiltration of explants during cultivation in diffusion chambers].

An extensive formation of epithelioid cell spheroids was shown during tumour tissue growth on millipore filters. In addition, the appearance of such multicellular structures as an early sign of malignant transformation was demonstrated in some fibroadenomatous tissue cultures. The increased fibroadenomatous tissue proliferative activity was accompanied by the rising level of lymphoid cell infiltration.

[Proliferative activity of cells in dyshormonal fibroadenomatosis of the human breast].

Fibroadenomatous tissue of the human mammary gland was cultivated in diffuse chambers implanted into mice. On day 6 of culture the growing cells were subjected to morphological and autoradiographic analysis. The index of 3H-thymidine labeling of cell nuclei was found to correlate with the morphological pattern of dyshormonal fibroadenomatosis of the mammary gland.

[Lymph node cell composition and DNA content in their different lymphocyte populations in mice with tumor induced by murine Moloney sarcoma virus].

The ratio of T and B lymphocytes and DNA content in population enriched by these cells were analysed in the lymph nodes of BALB/c mice with progressively growing sarcomas induced by the MSV Moloney and in untreated mice. T lymphocyte enriched fractions have a higher DNA content in comparison to fractions enriched with B lymphocytes. Sarcoma development promotes a decrease in the relative number of macronucleolar lymphocytes and simultaneous decrease of micronucleolar ones in the lymph nodes, particularly in the regional ones.

[DNA metabolism in the immunocompetent organs of C57BL mice in chemical carcinogenesis].

DNA synthesis was determined in small lymphocytes nuclei in mice C57BL during the process of methylcholanthrene induced carcinogenesis. Both in the pretumor period and in animals with a tumor the DNA synthesis in lymph node lymphocytes nuclei was found to be decreased. Inhibition of the DNA synthesis is greatly pronounced in lymphocytes adjacent to the site of lymph node tumor development.