Effect of glucosamine on interleukin-1-conditioned articular cartilage.

Glucosamine inhibits recombinant human interleukin-1 stimulated cartilage degradation in equine cartilage explants. Recently, recombinant equine interleukin-1 has been cloned and purified. Therefore, the objective of this study was to characterise the effects of glucosamine on indices of cartilage degradation in recombinant equine IL-1beta-stimulated equine articular cartilage explants.

The effects of glucosamine derivatives on equine articular cartilage degradation in explant culture.

Objective: To determine whether glucosamine-3-sulfate, glucose-3-sulfate (control) and N-acetyl glucosamine inhibit experimentally induced degradation of equine articular cartilage explants similar to glucosamine HCl.

Design: Articular cartilage was obtained from the antebrachio-carpal and middle joints of horses (2-8 years old) killed for reasons unrelated to lameness. Cartilage discs were harvested from the weight-bearing region of the articular surface and cultured.

Glucosamine HCl reduces equine articular cartilage degradation in explant culture.

Objective To determine whether glucosamine inhibits experimentally induced degradation of equine articular cartilage explants. Methods Articular cartilage was obtained from the antebrachio-carpal and middle joints of horses (2-8 years old) killed for reasons unrelated to lameness. Cartilage discs were harvested from the weight-bearing region of the articular surface and cultured.

Cartilage turnover in embryonic chick tibial explant cultures.

Growth plate cartilage regulates the rate of growth and ultimate length of several bones in the skeleton. Chondrocytes within the growth plate proliferate, differentiate, enlarge, and die. The extracellular matrix undergoes synthesis, reorganization, and eventually degradation.

Biochemical characterization of cartilage degradation in embryonic chick tibial explant cultures.

The growth plates of birds reared for meat production are susceptible to diseases such as tibial dyschondroplasia (TD). We have modified a tibial explant culture system to study the regulation of growth plate cartilage turnover. The purpose of these experiments was to characterize some of the biochemical changes that occur in cultured tibiae as the cartilage is degraded.