The effect of GV-58, a calcium channel modifier, on synaptic transmission at the larval and crayfish neuromuscular junctions.

GV-58 is known to increase the opening time of the mammalian P-type calcium channel in presynaptic motor nerve terminals. GV-58 is suggested as a therapeutic agent for dampening the symptoms of amyotrophic lateral sclerosis. To further understand the mechanisms of GV-58 actions, the and crayfish neuromuscular junctions were used as models.

Development of an H&E on-block staining technique for collagen detection in cryo-fluorescence tomography imaging of frozen breast tissue samples.

Background: Hematoxylin and eosin (H&E) staining is widely considered to be the gold-standard diagnostic tool for histopathology evaluation. However, the fatty nature of some tissue types, such as breast tissue, presents challenges with cryo-sectioning, often resulting in artifacts that can make histopathologic interpretation and correlation with other imaging modalities virtually impossible. We present an optimized on-block H&E staining technique that improves contrast for identifying collagenous stroma during cryo-fluorescence tomography (CFT) sectioning.

The Effect of Magnesium Concentration on Myogenic Cardiac Function: Larval .

The heart of larval serves as a model preparation in addressing cardiac function, as known genetic mutations can be mimicked to examine therapies. Pharmacological agents and function of proteins, like TRPA1, which affect ionic transport and ion concentrations can be investigated for their action on cardiac function in this model. To maintain function, the larval heart tube needs to remain viable; thus, a physiological saline is required.

Interview Results From a Telehealth Hospice Intervention for Children and Young Adults With Cancer.

Background And Aims: Surveyed families of children and young adults with cancer who participated in a pilot study of three coordinated telehealth visits (triad of patient/family, hospital clinician, and hospice nurse) during the first month of hospice enrollment found telehealth feasible and were highly satisfied with hospice care. The aim of this study is to further explore adult patient and caregiver perspectives on telehealth, specifically the benefits of telehealth, trade-offs with in-person appointments, and the impact on patient care and family end-of-life preparations.

Methods: This is a single-arm prospective pilot study of patients aged 0-29-with cancer initiating hospice care between 2021 and 2022 and their caregivers.