Genome-wide DNA methylation profiles regulate distinct heat stress response in zebu (Bos indicus) and crossbred (Bos indicus × Bos taurus) cattle.

Epigenetic variations result from long-term adaptation to environmental factors. The Bos indicus (zebu) adapted to tropical conditions, whereas Bos taurus adapted to temperate conditions; hence native zebu cattle and its crossbred (B indicus × B taurus) show differences in responses to heat stress. The present study evaluated genome-wide DNA methylation profiles of these two breeds of cattle that may explain distinct heat stress responses.

Formation and Transformation of Clathrate Hydrates under Interstellar Conditions.

ConspectusContinuing efforts by many research groups have led to the discovery of ∼240 species in the interstellar medium (ISM). Observatory- and laboratory-based astrochemical experiments have led to the discovery of these species, including several complex organic molecules (COMs). Interstellar molecular clouds, consisting of water-rich icy grains, have been recognized as the primordial sources of COMs even at extremely low temperatures (∼10 K).

Induced Migration of CO from Hydrate Cages to Amorphous Solid Water under Ultrahigh Vacuum and Cryogenic Conditions.

Restricted migration of reactive species limits chemical transformations within interstellar and cometary ices. We report the migration of CO from clathrate hydrate (CH) cages to amorphous solid water (ASW) in the presence of tetrahydrofuran (THF) under ultrahigh vacuum (UHV) and cryogenic conditions. Thermal annealing of sequentially deposited CO and HO ice, CO@HO, to 90 K resulted in the partitioning of CO in 5 and 56 CH cages (CO@5, CO@56).

Machine Learning in Drug Metabolism Study.

Metabolic reactions in the body transform the administered drug into metabolites. These metabolites exhibit diverse biological activities. Drug metabolism is the major underlying cause of drug overdose-related toxicity, adversative drug effects and the drug's reduced efficacy.

Accelerated and Concerted Aza-Michael Addition and SuFEx Reaction in Microdroplets in Unitary and High-Throughput Formats.

The sulfur fluoride exchange (SuFEx) reaction is significant in drug discovery, materials science, and chemical biology. Conventionally, it involves installation of SO F followed by fluoride exchange by a catalyst. We report catalyst-free Aza-Michael addition to install SO F and then SuFEx reaction with amines, both occurring in concert, in microdroplets under ambient conditions.

Facile Synthesis of Triazoles using Electrospray-Deposited Copper Nanomaterials to Catalyze Azide-Alkyne Cycloaddition (AAC) Click Reactions.

Electrospray deposition of copper salt-containing microdroplets onto the liquid surface of an electrically grounded reaction mixture leads to the formation of Cu nanoclusters, which then catalyze the azide-alkyne cycloaddition (AAC) reaction to form triazoles. This method of in situ nanocatalyst preparation provided 17 times higher catalytic activity compared to that in the conventional catalytic reaction. The gentle landing of the Cu-containing droplets onto the liquid surface forms a thin film of catalyst which promotes the heterogeneous AAC reaction while showing diffusion-controlled kinetics.

Late-Stage Functionalization and Characterization of Drugs by High-Throughput Desorption Electrospray Ionization Mass Spectrometry.

Late-stage functionalization (LSF) of drug molecules is an approach to generate modified molecules that retain functional groups present in the active drugs. Here, we report a study that seeks to characterize the potential value of high-throughput desorption electrospray ionization mass spectrometry (HT DESI-MS) for small-scale rapid LSF. In conventional route screening, HT-based DESI-MS provides contactless, rapid analysis, reliable and reproducible data, minimal sample requirement, and exceptional tolerance to high salt concentrations.

Desorption-induced evolution of cubic and hexagonal ices in an ultrahigh vacuum and cryogenic temperatures.

Reflection absorption infrared spectroscopic investigations of multilayer films of acetonitrile (ACN) and water in an ultrahigh vacuum under isothermal conditions showed the emergence of cubic (ice I) and hexagonal (ice I) ices depending on the composition of the film. The experiments were conducted with a mixed film of 300 monolayers in thickness and the ACN : HO monolayer ratios were varied from 1 : 5 to 5 : 1. Mixed films were deposited at 10 K and warmed to 130-135 K, where ACN desorbed subsequently and IR spectral evolution was monitored continuously.

Organic Zinc and Copper Supplementation-Associated Changes in Gene Expression and Protein Profiles in Buck Spermatozoa.

Mineral supplementation has greater impact on male reproduction; however, the mechanism of action has not been studied in detail. The present study was aimed to deal with the lacuna in mechanism of action of mineral supplementation on improvement in sperm characteristics. A group of 40 bucks (aged 5 months) were assigned to 10 groups (4 in each group) based on their body weight and fed with concentrate mixture: basal roughage (minimal diet) in equal proportion to all the bucks.

Sociodemographic and clinical profile of drug treatment seekers attending the State Psychiatric Hospital and Drug De-addiction Center at Agartala, Tripura.

Background: Substance use disorders have become a widespread public health problem, especially in the northeastern states, and limited studies have been undertaken to reflect the picture of the same.

Aims: The aim of the study was to study the sociodemographic and clinical profile of drug treatment seekers attending a State Psychiatric Hospital and De-addiction Center of Northeastern India.

Materials And Methods: It was a cross-sectional hospital-based study conducted from October 2018 to August 2019.

Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media.

Multipotent porcine mesenchymal stem cells (pMSC) are invaluable for research and therapeutic use in regenerative medicine. Media used for derivation and expansion of pMSC may play an important role for the selection of MSC subpopulation at an early stage and thereby, the specific basal medium may also affect differentiation potential of these cells. The present study was undertaken to evaluate the effects of αMEM, aDMEM, M199, αMEM/M199, aDMEM/M199 and αMEM/aDMEM media on (1) porcine bone marrow MSC derivation; (2) expression of number of osteogenic markers (ALP, COL1A1, SPP1 and BGLAP) at 5th and 10th passage in pMSC before differentiation; and (3) differentiation of pMSC (at 5th passage) to osteogenic lineage.

Iron assisted formation of CO over condensed CO and its relevance to interstellar chemistry.

Catalytic conversion of CO to CO has been investigated in ultrahigh vacuum (UHV) under cryogenic conditions (10 K). This cryogenic oxidation is assisted by iron upon its co-deposition with CO, on a substrate. The study shows that the interaction of Fe and CO results in a Fe-CO complex that reacts in the presence of excess CO at cryogenic conditions leading to CO.

Nonenzymatic Glucose Sensing Using NiNb Nanoglass.

Despite being researched for nearly five decades, chemical application of metallic glass is scarcely explored. Here we show electrochemical nonenzymatic glucose-sensing ability of nickel-niobium (NiNb) amorphous alloys in alkaline medium. Three different NiNb systems with the same elemental composition, but varying microstructures are created following different synthetic routes and tested for their glucose-sensing performance.

Buffalo early pregnancy biomarker coding sequence cloning and partial length expression in after codon optimization.

Pregnancy-associated glycoproteins (PAGs) secreted from conceptus specific trophoblast cells are widely accepted biomarkers of ruminants. Limited information of PAGs in buffalo warrants further investigation for the development of sensitive homologous early pregnancy-specific diagnostic immunoassay. This experiment was thus designed to identify and clone the predominantly expressed early placentome-specific buffalo PAG (buPAG) isoform; to express this PAG isoform and verify its antigenicity by developing antisera and testing immuno-reactivity with recombinant proteins.

Formation of Cubic Ice via Clathrate Hydrate, Prepared in Ultrahigh Vacuum under Cryogenic Conditions.

Cubic ice (ice I) is a crystalline phase of solid water, which exists in the earth's atmosphere and extraterrestrial environments. We provide experimental evidence that dissociation of acetone clathrate hydrate (CH) makes ice I in ultrahigh vacuum (UHV) at 130-135 K. In this process, we find that crystallization of ice I occurs below its normal crystallization temperature.

Lupeol alters viability of SK-RC-45 (Renal cell carcinoma cell line) by modulating its mitochondrial dynamics.

Renal cell carcinoma (RCC) is the most common kidney cancer leading to 140,000 deaths per year. Among all RCCs 80% evolve from the epithelial proximal tubular cells within the kidney. There is a high tendency of developing chemoresistance and resistance to radiation therapy in most RCC patients.

Clathrate hydrates in interstellar environment.

Clathrate hydrates (CHs) are ubiquitous in earth under high-pressure conditions, but their existence in the interstellar medium (ISM) remains unknown. Here, we report experimental observations of the formation of methane and carbon dioxide hydrates in an environment analogous to ISM. Thermal treatment of solid methane and carbon dioxide-water mixture in ultrahigh vacuum of the order of 10 mbar for extended periods led to the formation of CHs at 30 and 10 K, respectively.

Partial purification and characterization of phytase from Aspergillus foetidus MTCC 11682.

Phytase is a phosphatase enzyme widely used as feed additive to release inorganic phosphorus from plant phytate and enhance its uptake in monogastric animals. Although engineered fungal phytases are used most, a natural enzyme gives opportunity to understand novel properties, if any. In the current study, a novel fungal strain, Aspergillus foetidus MTCC 11682 was immobilized on poly urethane cubes and used for phytase production, purification and molecular characterization.

A thirty-fold photoluminescence enhancement induced by secondary ligands in monolayer protected silver clusters.

In this paper, we demonstrate that systematic replacement of the secondary ligand PPh3 leads to an enhancement in the near-infrared (NIR) photoluminescence (PL) of [Ag29(BDT)12(PPh3)4]3-. While the replacement of PPh3 with other monophosphines enhances luminescence slightly, the replacement with diphosphines of increasing chain length leads to a drastic PL enhancement, as high as 30 times compared to the parent cluster, [Ag29(BDT)12(PPh3)4]3-. Computational modeling suggests that the emission is a ligand to metal charge transfer (LMCT) which is affected by the nature of the secondary ligand.

Efficacy of dietary phytase supplementation on laying performance and expression of osteopontin and calbindin genes in eggshell gland.

This study was conducted to evaluate the effects of different levels of dietary phytase supplementation in the layer feed on egg production performance, egg shell quality and expression of osteopontin () and calbindin () genes. Seventy-five White Leghorn layers at 23 weeks of age were randomly divided into 5 groups consisting of a control diet with 0.33% non-phytate phosphorus (NPP) and 4 low phosphorus (P) diets: 2 diets (T1 and T2) with 0.

Cigarette Smoke Toxins-Induced Mitochondrial Dysfunction and Pancreatitis Involves Aryl Hydrocarbon Receptor Mediated Cyp1 Gene Expression: Protective Effects of Resveratrol.

We previously reported that mitochondrial CYP1 enzymes participate in the metabolism of polycyclic aromatic hydrocarbons and other carcinogens leading to mitochondrial dysfunction. In this study, using Cyp1b1-/-, Cyp1a1/1a2-/-, and Cyp1a1/1a2/1b1-/- mice, we observed that cigarette and environmental toxins, namely benzo[a]pyrene (BaP) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), induce pancreatic mitochondrial respiratory dysfunction and pancreatitis. Our results suggest that aryl hydrocarbon receptor (AhR) activation and resultant mitochondrial dysfunction are associated with pancreatic pathology.

Effect of immobilized fungal phytase on growth performance and bone traits of broilers fed with low dietary calcium and phosphorus.

Aim: The aim of this study was to investigate the effects of phytase which was laboratory produced by on the growth performance, mineral retention, and bone traits of broilers fed with low dietary calcium and phosphorus.

Materials And Methods: The extracellular phytase enzyme secreted into the crude filtrate was concentrated by ammonium sulfate precipitation to obtain an activity of 500 phytase units (FTU). A total of 90 1-day-old chicks (Cobb 500) were randomly divided into three treatment groups with five replicates having six birds each.

Rapid reaction of MoS nanosheets with Pb and Pb ions in solution.

Understanding the chemical changes happening to nanostructures during a process is vital in selecting them for applications. Here, we investigated the difference in the reactivity of the bulk and nanoscale forms of molybdenum disulfide (MoS) in solution with lead ions (Pb and Pb) as probes, at room temperature. While the bulk form did not show any reactivity in the experimental timescale, the two-dimensional (2D) nanoscale form showed not only reactivity but also quite rapid kinetics that resulted in the formation of distinct products, principally PbMoO with anion substitution, in a few seconds.

Propane and propane-water interactions: a study at cryogenic temperatures.

The phase transition of solid propane and a propane-water mixture under ultrahigh vacuum has been investigated using reflection absorption infrared spectroscopy (RAIRS) and temperature-programmed desorption mass spectrometry (TPD-MS). Here, the investigation is divided into two sections: the phase transition of pure propane and the interaction of propane with water. RAIR spectra of pure propane reveal an unknown crystalline phase at 50 K (phase I), which gradually converts to a known crystalline phase (phase II) at higher temperature.