The beer and biofuels laboratory: A report on implementing and supporting a large, interdisciplinary, yeast-focused course-based undergraduate research experience.

Course-integrated Undergraduate Research Experiences (CUREs) involve large numbers of students in real research. We describe a late-year microbiology CURE in which students use yeast to address a research question around beer brewing or synthesizing biofuel; the interdisciplinary student-designed project incorporates genetics, bioinformatics, biochemistry, analytical chemistry, and microbiology. Students perceived significant learning gains around multiple technical and "becoming a scientist" aspects of the project.

A Comparison of the Selectivity of Extraction of [PtCl6](2-) by Mono-, Bi-, and Tripodal Receptors That Address Its Outer Coordination Sphere.

Extraction and binding studies of [PtCl6](2-) are reported for 24 mono-, bi-, and tripodal extractants containing tris(2-aminoethyl)amine (TREN) or tris(3-aminopropyl)amine (TRPN) scaffolds. These reagents are designed to recognize the outer coordination sphere of [PtCl6](2-) and to show selectivity over chloride anion under acidic conditions. Extraction from 0.

Design and function of pre-organised outer-sphere amidopyridyl extractants for zinc(II) and cobalt(II) chlorometallates: the role of C-H hydrogen bonds.

Four new sterically hindered pyridines, L(1)-L(4)-containing amido substituents at the 2-position act as efficient solvent extractants for [CoCl(4)](2-) or [ZnCl(4)](2-) from acidic chloride solutions through protonation of the pyridino N-centre to form the neutral outer-sphere complexes [(LH)(2)MCl(4)]. These ionophores show very high selectivity for chlorometallate anions over chloride ion and are readily stripped to liberate the free-metal chlorides without the formation of inner-sphere complexes [ML(2)Cl(2)]. Single-crystal X-ray structure determinations of [(L(2)H)(2)CoCl(4)] and [(L(2)H)(2)ZnCl(4)] (L(2) = 2-(4,6-di-tert-butylpyridin-2-yl)-N,N'-dihexylmalonamide) coupled with (1)H NMR spectroscopy and DFT calculations on L(2)H(+) and other complexes of [ZnCl(4)](2-) confirm that the pyridinium NH group does not address the outer co-ordination sphere of the metallanion, but rather forms a hydrogen bond to the pendant amide groups and thus pre-organizes the ligand to present both C-H and amido N-H hydrogen-bond donors to the [MCl(4)](2-) ions.

Copper redistribution in murine macrophages in response to Salmonella infection.

The movement of key transition metal ions is recognized to be of critical importance in the interaction between macrophages and intracellular pathogens. The present study investigated the role of copper in mouse macrophage responses to Salmonella enterica sv. Typhimurium.

Heterocyclic dithiocarbazate iron chelators: Fe coordination chemistry and biological activity.

The iron coordination and biological chemistry of a series of heterocyclic dithiocarbazate Schiff base ligands is reported with regard to their activity as Fe chelators for the treatment of Fe overload and also cancer. The ligands are analogous to tridentate heterocyclic hydrazone and thiosemicarbazone chelators we have studied previously which bear NNO and NNS donor sets. The dithiocarbazate Schiff base ligands in this work also are NNS chelators and form stable low spin ferric and ferrous complexes and both have been isolated.

A turn-on fluorescent iron complex and its cellular uptake.

In the treatment of chronic iron overload disorders, ligands capable of complexing so-called "labile" (nonprotein bound) Fe are required to enter iron-loaded cells, sequester excess Fe, and then exit the cell (and the body) as an intact Fe complex. Despite the emergence of several ligand families that show high activity in mobilizing intracellular Fe, the mechanism and the locations of these subcellular labile Fe pools are still poorly understood. Our previous studies have unearthed a class of heterocyclic hydrazine-based chelators (e.

An unusually flexible expanded hexaamine cage and its Cu(II) complexes: variable coordination modes and incomplete encapsulation.

The bicyclic hexaamine "cage" ligand Me(8)tricosaneN(6) (1,5,5,9,13,13,20,20-octamethyl-3,7,11,15,18,22-hexaazabicyclo[7.7.7]tricosane) is capable of encapsulating octahedral metal ions, yet its expanded cavity allows the complexed metal to adopt a variety of geometries comprising either hexadentate or pentadentate coordination of the ligand.

Selective extraction and transport of the [PtCl6]2- anion through outer-sphere coordination chemistry.

A series of tripodal receptors designed to recognise the outer coordination sphere of the hexachlorometallate anion [PtCl(6)](2-), and thus show selectivity for ion-pair formation over chloride binding, has been synthesised and characterised. The tripodal ligands contain urea, amido or sulfonamido hydrogen-bond donors, which are aligned to bind to the regions of greatest electron density along the faces and edges of the octahedral anion. The ligand structure incorporates a protonatable bridgehead nitrogen centre that provides a positive charge to ensure the solubility of a neutral 2:1 [LH](+)/[PtCl(6)](2-) complex in water-immiscible solvents.

Outer-sphere amidopyridyl extractants for zinc(ii) and cobalt(ii) chlorometallates.

Protonated amidopyridyl ligands show high selectivity for the extraction of [CoCl(4)](2-) or [ZnCl(4)](2-) over chloride ion into organic media via the formation of N-H and C-H hydrogen-bond donors to the outer coordination spheres of the chlorometallates.

A second generation dendrimer incorporating nine S2N2-donor macrocycles and its palladium(II) complex.

A new second generation dendrimer incorporating nine S2N2-donor macrocyclic units that bind nine Pd(II) cations is reported.