Occurrence and potential risks of pharmaceutical contamination in global Estuaries: A critical review and analysis.

Input of pollutants to estuaries is one of the major threats to marine biodiversity and fishery resources, and pharmaceuticals are one of the most important contaminants of emerging concern in aquatic ecosystems. To synthesize pharmaceutical pollution levels in estuaries over the past 20 years from a global perspective, this review identified 3229 individual environmental occurrence data for 239 pharmaceuticals across 91 global estuaries distributed in 26 countries. The highest cumulative weighted average concentration level (WACL) of all detected pharmaceuticals in estuarine water was observed in Africa (145,461.

The NAC gene family in the halophyte Limonium bicolor: Identification, expression analysis, and regulation of abiotic stress tolerance.

NAC transcription factors regulate plant growth, development, and stress responses. However, the number, types, and biological functions of Limonium bicolor LbNAC genes have remained elusive. L.

Different responses of marine microalgae Phaeodactylum tricornutum upon exposures to WAF and CEWAF of crude oil: A case study coupled with stable isotopic signatures.

Increasing use of chemical dispersants for oil spills highlights the need to understand their adverse effects on marine microalgae and nutrient assimilation because the toxic components of crude oil can be more bioavailable. We employed the crude oil water-accommodated fraction (WAF) and chemically enhanced WAF (CEWAF) to compare different responses in marine microalgae (Phaeodactylum tricornutum) coupled with stable isotopic signatures. The concentration and proportion of high-molecular-weight polycyclic aromatic hydrocarbons (HMW PAHs), which are key toxic components in crude oil, increased after dispersant addition.

Integrated transcriptome and metabolome analysis revealed that HaMYB1 modulates anthocyanin accumulation to deepen sunflower flower color.

HanMYB1 was found to play positive roles in the modulation of anthocyanins metabolism based on the integrative analysis of different color cultivars and the related molecular genetic analyses. As a high value ornamental and edible crop with various colors, sunflowers (Helianthus annuus L.) provide an ideal system to understand the formation of flower color.

Contrasting effects of temperature and biological processes on aragonite saturation state at the A4HDYD station in the North Yellow Sea.

To explore the effects of seawater temperature and biological processes on aragonite saturation state (Ω), eight field surveys were conducted from December 2014 to November 2015 at A4HDYD station in the North Yellow Sea (NYS). Low regional yearly initial values of seawater Ω (1.26-1.

Nutrient changes in the Bohai Sea over the past two decades.

With the growing problem of eutrophication in the Bohai Sea, actions have been taken to reduce nutrient inputs, but it remains to be seen whether nutrient levels and structure have been ameliorated. In this study, the nutrient trends in the Bohai Sea are re-examined based on observations from 2000 to 2019. The results suggest that dissolved inorganic nitrogen (DIN) concentrations and DIN/DIP (dissolved inorganic phosphate) ratios gradually increased from 2000 to 2013 but dramatically decreased from 2013 to 2019.

Seasonal distribution of microplastics in the surface waters of the Yellow Sea, China.

Different studies are filling the gaps in the distribution map of global marine microplastics. However, the data on the seasonal variation is relatively limited, which may lead to overestimation or underestimation of the distribution level of microplastics. To understand baseline data and seasonal variations of the microplastics in the surface seawater of the Yellow Sea, a survey over four seasons was conducted during 2017-2018.

Insights into the Antifungal Properties of Myxobacteria Outer Membrane β-1,6-Glucanase.

Fungal cell wall decomposition enzymes exhibit great potential for the development of efficient antifungal agents. However, their practical application is restricted due to incomplete understanding of the action mode. In our previous study, we identified that a novel outer membrane (OM) β-1,6-glucanase GluM is deployed by predatory myxobacteria to feed on fungi.

Bisphenol A decreases the developmental toxicity and histopathological alterations caused by polystyrene nanoplastics in developing marine medaka Oryzias melastigma.

Nanoplastics (NPs) are emerging pollutants posing risks to marine biota and human health due to their small size and high bioavailability. However, there are still knowledge gaps regarding effects of co-existing pollutants on NPs toxicity to marine organisms at their respective environmentally relevant concentrations. Herein we investigated developmental toxicity and histopathological alterations caused by co-exposure of polystyrene nanoplastics (PS-NPs) and bisphenol A (BPA) to marine medaka, Oryzias melastigma.

Predation of oomycetes by myxobacteria via a specialized CAZyme system arising from adaptive evolution.

As social micropredators, myxobacteria are studied for their abilities to prey on bacteria and fungi. However, their predation of oomycetes has received little attention. Here, we show that Archangium sp.

Mechanism of Notch Signal Regulating Alveolar Epithelial Cell Autophagy after Infection with Klebsiella Pneumoniae.

Background: The Notch signaling pathway regulates various cellular processes, including cell growth, inflammation response, and autophagy, thereby participating in the occurrence and development of various diseases. The present study aimed to investigate the molecular mechanism of Notch signaling in regulating alveolar type II epithelial cell viability and autophagy after Klebsiella pneumonia (KPN) infection.

Methods: KPN-infected human alveolar type II epithelial cells A549 (ACEII) were constructed.

Heterologous overexpression of flavonoids synthetase genes improves salt tolerance by activating the IAA and JA biosynthesis pathways.

Salt stress is a serious abiotic stress that primarily inhibits plant growth, resulting in severe yield losses. Our previous research found that flavonoids play important roles in salt stress tolerance. In response to salt stress, we noted that the flavonoid content was depleted in .

Acute and Chronic Effects of Crude Oil Water-Accommodated Fractions on the Early Life Stages of Marine Medaka (, McClelland, 1839).

Oil spill is a major marine environmental pollution issue. Research regarding the long-term effects of oil spills on the early life stage of marine fish is still limited. In this study, the potential adverse impact of crude oil from one oil spill accident which occurred in the Bohai Sea on the early life stages of marine medaka (, McClelland, 1839) was evaluated.

Polystyrene microplastics alleviate adverse effects of benzo[a]pyrene on tissues and cells of the marine mussel, Mytilus galloprovincialis.

As two major ubiquitous pollutants, microplastics (MPs) and polycyclic aromatic hydrocarbons (PAHs) coexist in the marine environment. However, the role of MPs in altering the toxicity of PAHs to marine organisms is poorly understood. We therefore investigated the accumulation and toxicity of benzo[a]pyrene (B[a]P, 0.

Empirical Analysis of Customer Risk and Corporate Financing Constraints Based on Supply Chain Networks.

A supply chain's risk spillover effect will affect the customer's risk on the financing constraints of suppliers. This paper builds on the evaluation of customer risk by fuzzy mathematics, combines with the A-share listed companies in Shanghai and Shenzhen from 2007 to 2019 as a study sample, and empirically inspects the influence of customer risk on the level of corporate financing constraints. According to the study, it shows that the customer risk is currently at a moderate level, which will notably impair the supplier's external financing ability.

Distribution characteristics of microplastics in surface and subsurface Antarctic seawater.

Microplastics have attracted worldwide attention due to their potential threat to the marine ecosystem, with such pollutants even detected in the polar seas. Although in-depth research on microplastics has increased in recent years, studies in Antarctic waters remain relatively scarce compared with coastal waters and open oceans. In this study, microplastics in surface and subsurface Antarctic waters were investigated.

Systematical insights into distribution and characteristics of microplastics in near-surface waters from the East Asian Seas to the Arctic Central Basin.

The spatial distribution and composition of microplastics in near-surface water (8 m) was investigated from the East Asian Seas to the Arctic Central Basin. Microplastics were detected in 93.9% of the sampling sites.

Adenoviral Transduction of Alleviates Silica-Induced Silicosis Development in Lungs of Mice.

Silicosis is an occupational disease caused by inhalation of silica dust, which is hallmarked by progressive pulmonary fibrosis associated with poor prognosis. Wnt/β-catenin signaling is implicated in the development of fibrosis and is a therapeutic target for fibrotic diseases. Previous clinical studies of patients with pneumoconiosis, including silicosis, revealed an increased concentration of circulating WNT3A and DKK1 proteins and inflammatory cells in bronchoalveolar lavage compared with healthy subjects.

Structural and digestion properties of potato starch modified using an efficient starch branching enzyme AqGBE.

Modified potato starch with slower digestion may aid the development of new starch derivatives with improved nutritional values, and strategies to increase nutritional fractions such as resistant starch (RS) are desired. In this study, a correspondence between starch structure and enzymatic resistance was provided based on the efficient branching enzyme AqGBE, and modified starches with different amylose content (Control, 100%; PS1, 90%; PS2, 72%; PS3, 32%; PS4, 18%) were prepared. Through SEM observation, NMR and X-ray diffraction analyses, we identified that an increased proportion of α-1,6-linked branches in potato starch changes its state of granule into large pieces with crystallinity.

Lethal, behavioral, growth and developmental toxicities of alkyl-PAHs and non-alkyl PAHs to early-life stage of brine shrimp, Artemia parthenogenetica.

Alkyl-PAHs are the predominant form of PAHs in crude oils which are supposed to demonstrate different toxicities compared to non-alkyl PAHs. Little information is available about the toxicity of alkyl-PAHs on marine Artemia. This study addressed and compared the lethal, behavioral, growth and developmental toxicities of three alkyl-PAHs, namely 3-methyl phenanthrene (3-mPhe), retene (Ret) and 2-methyl anthracene (2-mAnt), to their non-alkyl forms, phenanthrene (Phe) and anthracene (Ant) using Artemia parthenogenetica (nauplii, <24 h) as test organism following a 48 h and a 7 d of exposure, respectively.

The importance of preoperative T1 slope for determining proper postoperative C2-7 Cobb's angle in patients undergoing cervical reconstruction.

Background: This study aimed to explore the relationship among different cervical sagittal parameters in asymptomatic volunteers and the correlation between surgical efficacy and difference of presumed and actual postoperative C2-7 Cobbs's angle (C2-7COBB), which was calculated based on preoperative T1 slope (T1S) in patients undergoing cervical reconstruction.

Methods: In total, 158 inpatients with cervical spondylosis and 274 asymptomatic volunteers were retrospectively reviewed. Cervical sagittal parameters, such as C2-7COBB, T1S, thoracic inlet angle (TIA), and neck tilt (NT), were compared.

Spatio-temporal distribution of plastic and microplastic debris in the surface water of the Bohai Sea, China.

As an emerging marine environmental issue, marine plastic debris pollution has attracted worldwide attention. Studies have covered more and more areas of the world's oceans. To further understand the sources and variation of marine plastic debris in the surface water of the Bohai Sea, in this study, plastic debris was collected during the four seasons of 2016-2017.

Comparative Studies on Bioactive Compounds, Ganoderic Acid Biosynthesis, and Antioxidant Activity of Pileus and Stipes of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes) Fruiting Body at Different Growth Stages.

Total phenolics, flavonoids, and polysaccharides, and individual ganoderic acid (GA) contents, antioxidant capacity, and transcription levels of key enzyme genes involved in GA biosynthesis in pileus and stipes of Ganoderma lucidum fruiting body at different growth stages were investigated in this study. Results showed that the highest total phenolics and total flavonoids contents were determined in stipes at spore maturity stage, resulting in high antioxidant activity, while the highest total polysaccharide content was found in pileus at the same stage. The pileus contained more GA than the stipes, and higher contents of ganoderic acid A and D were found at fruiting body mature stage while that of ganoderic acid B, C2, and G were found at bud elongation stage.

Methane emissions from oil and gas platforms in the Bohai Sea, China.

Although oil and gas explorations contribute to atmospheric methane (CH) emissions, their impact and influence along the shelf seas of China remain poorly understood. From 2012 to 2017, we conducted four ship-based surveys of CH in the seawater column and boundary layer of the Bohai Sea, China, and further measured CO and several meteorological parameters. The average observed CH mixing ratios in the boundary layer and its concentrations in seawater column were 1950 ± 46 ppb in November 2012 (dissolved CH was not observed in this survey), 2222 ± 109 ppb and 13.

Multiple factors dominate the distribution of methane and its sea-to-air flux in the Bohai Sea in summer and autumn of 2014.

The Bohai Sea is well-known as a source of atmospheric methane (CH). However, the main regulate factors of the spatiotemporal distribution of CH and its sea-to-air flux remain largely unknown. In this study, the observed CH concentration ranged from 4.