Spleen migrating dendritic cells primed with CC531 colon cancer antigen and LPS - is it a method to compromise liver metastases?

The anti-tumor vaccination is burdened by low recruitment rate of intravenously administered in vitro primed DC in liver metastases and lack of supplying them continuously in large numbers. Therefore, it seemed rational to create a model of in vivo vaccination with specifically primed splenic DC and cytotoxic T lymphocytes being continuously supplied to the liver vascular bed. The question we raised was whether anti-tumor immunized splenic DC flowing to liver metastases could adhere to and be cytotoxic to tumor cells.

The response of spleen dendritic cell-enriched population to bacterial and allogeneic antigens.

The dendritic cells (DC) play crucial role in initiation and modulation of immune response especially innate immune response. We investigated the influence bacterial (E. coli and S.

Influence of bacterial antigens on activation of human splenic dendritic cells.

The dendritic cells (DC) play crucial role in initiation and modulation of immune response especially innate immune response. Toll like receptors (TLR) on DC are receptors involved in innate immunity and recognizing conserved bacterial antigens like LPS and bacterial DNA. TLRs can also respond to some endogenous ligands (heat-shock proteins, heparan sulfate, fibrinogen and the contest of necrotic cells).

Spleen dendritic cells of recipients of allogeneic but not syngeneic heart grafts internalize donor DNA fragments.

Microchimerism after allogeneic organ transplantation has been widely documented using DNA identification techniques. However, the question as to whether the detected donor DNA is present in the surviving donor cells, recipient macrophages phagocytizing rejected donor cells or recipient dendritic cells internalizing donor apoptotic bodies or cell fragments has not been answered. We provide evidence that allogeneic organ transplantation is followed not only by cellular microchimerism caused by release of graft passenger cells but also dissemination of donor DNA from the rejecting graft cells and its internalization in recipient dendritic cells (DC).

RNA interference--significance and applications.

RNA interference (RNAi) is a post-transcriptional, highly conserved process in eukaryotes that leads to specific gene silencing through degradation of the target mRNA. This mechanism is mediated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. The dsRNA is processed into small interfering RNA (siRNA) by an enzyme called Dicer, and the siRNAs are then incorporated into a multi-component RNA-induced silencing complex, which finds and cleaves the target mRNA.