Participation of Selected Soluble BMP-2 and BMP-7 Bone Morphogenetic Proteins and Their Soluble Type I ALK-1 and Type II BMPR2 Receptors in Formation and Development of Endometriosis.

Angiogenesis is considered to be one of the key stages in the development of endometriosis. Recent studies indicate that bone morphogenetic proteins (BMPs) and their receptors (BMPR) may play an important role in the angiogenesis process. In the literature, however, there is a lack of publications concerning binding BMPs and their receptors with the pathogenesis of endometriosis.

Participation of selected soluble cell adhesion molecules and syndecans in formation and development of endometriosis.

Objectives: Concentrations of soluble ICAM-2, -3, -4 and syndecan-1 and -4 have not yet been marked in the peritoneal fluid of women with endometriosis. The aim of the study was to determine whether these molecules can participate in formation and development of endometriosis.

Material And Methods: The study comprised of 80 women at the proliferative phase of the menstrual cycle, aged 21 to 49 years (mean age 31.

The immune complex p53 protein/anti-p53 autoantibodies in the pathogenesis of ovarian serous carcinoma.

Objectives: The tests conducted were intended to analyze the concentration of p53 protein and anti-p53 autoantibodies in serum of women with ovarian tumours.

Material And Methods: The study included patients with diagnosed ovarian cancer: Cystadenoma serosum or Cystadenocarcinoma papillare serosum at IIIc stage (including 10 women who had G1, 14 women who had G2 and 30 women who had G3 staging). Concentrations of parameters were measured by ELISA.

The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis.

Purpose: Transforming growth factor β (TGF-β) is one of the major immune and inflammation factors responsible for regulating cell proliferation, differentiation, angiogenesis, and immune responses. Deregulated TGF-β activity, especially its influence in peritoneal cytokine cross-talk, has been implicated in pathologies of endometriosis. The aim of this study was to determine whether TGF-β could be involved in the pathogenesis of endometriosis.

The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosis.

Purpose: The purpose of the work was to evaluate possible associations between the complement components C1q, mannose-binding lectin (MBL) and C1 inhibitor (C1INH) with pathogenesis of endometriosis.

Methods: Concentrations of C1q, MBL and C1INH were measured by ELISA in peritoneal fluid (PF) in 80 women with or without endometriosis.

Results: Significantly higher PF levels of C1q, MBL and C1INH in women with endometriosis compared to control group were observed (p < 0.

The Delicate Balance between the Good and the Bad IL-1 Proinflammatory Effects in Endometriosis.

Background: Endometriosis is an inflammatory gynaecological disease with an associated chronic inflammation. Interleukin(IL)-1 is one of the most important immune and proinflammatory factors, produced mainly by monocytes and macrophages. Studies indicate the role of the cytokine from IL-1 family in endometrium-related disorders, particularly in endometriosis.

Assessment of concentrations of sTRAIL ligand and its receptors sTRAIL-R1 and sTRAIL-R2 - markers monitoring the course of the extrinsic pathway of apoptosis induction: potential application in ovarian cancer diagnostics.

Introduction: TNF-related apoptosis-inducing ligand (TRAIL) together with its receptors are involved in activation of the extrinsic pathway of apoptosis. Due to the special role of the apoptosis pathway in pathogenesis of ovarian cancers, the aim of the study was to assess concentrations of sTRAIL, sTRAIL-R1 and sTRAIL-R2 in serum of affected women.

Material And Methods: The study group included 85 women with diagnosed ovarian tumors: 35 women with ovarian serous cystadenoma, 15 women with ovarian teratoma and 35 women with serous cystadenocarcinoma.

Abnormal peritoneal regulation of chemokine activation-The role of IL-8 in pathogenesis of endometriosis.

Problem: Endometriosis is a chronic inflammatory disease associated with an impairment in immune response. Disorders in the peritoneal fluid and ectopic endometrium macrophage populations and their secretory products create a specific microenvironment inducing the development of the disease. The important factors involved in inflammation associated with endometriosis are chemokines, especially interleukin (IL)-8.

Effect of Th1/Th2 cytokine administration on proinflammatory SKOV-3 cell activation.

Introduction: Interleukin(IL)-1β, IL-6 and IL-12 might associate with inflammatory processes in a tumor progression and create a specific microenvironment for tumor growth. The aim of the study was to assess whether the Th1 and Th2 type cytokines, such as IL-2 and IL-10, affect ovarian carcinoma continuous cell line (SKOV-3) pro-inflammatory activation.

Material And Methods: SKOV-3 ovarian cells and peripheral blood mononuclear cells (PBMCs) were stimulated by IL-2 and IL-10.

Association of the Precursor of Interleukin-1β and Peritoneal Inflammation-Role in Pathogenesis of Endometriosis.

Background: The most important proinflammatory cytokine is interleukin (IL)-1β, however its precursor, prointerleukin-1β (proIL-1β), can also potentiate inflammatory state. The aim of this study was to explore the involvement of proIL-1β in pathogenesis of endometriosis. For this purpose, we evaluated concentrations of proIL-1β, IL-1β, and soluble IL-1 receptor type 2 (sIL-1R2) in peritoneal fluid (PF) and macrophage culture medium of women with endometriosis.

Peripheral blood proinflammatory response in women during menstrual cycle and endometriosis.

The aim of this study was to evaluate differences in levels of serum and monocyte derived interleukin (IL)-1, IL-6 and neopterin (NPT) in women with normal or abnormal menstrual cycles and women with endometriosis. The women participating in this study were divided into 4 groups: 25 women with normal menstrual cycle; 25 women taking oral contraception (OC); 20 postmenopausal women and 25 endometriosis patients. IL-1beta, IL-6 and NPT levels in serum and monocyte culture media were measured with ELISA methods.

Higher serum levels of tumour necrosis factor and its soluble receptors are associated with ovarian tumours.

Introduction: Tumour necrosis factor (TNF) and its soluble receptors type 1 (sTNF-R1) and type 2 (sTNF-R2) have been suggested as key mediators between apoptosis and cancer cell progression. The aim was to examine concentrations of the parameters in the serum of women with ovarian tumour and in the fluid from ovarian cysts of women with serous cystadenoma.

Material And Methods: The study included 125 women with ovarian tumours.

Imbalance in serum soluble CD30/CD30L and CD40/CD40L systems are associated with ovarian tumors.

The aim of the study was to examine the concentrations of the soluble receptors and their ligands of CD30/CD30L and CD40/CD40L systems in the serum of women with ovarian tumor and in the ovarian cyst fluid of women with Cystadenoma serosum. The study included 120 women with ovarian tumors. As a control, sera were obtained from 60 healthy female volunteers.

[Systemic lupus erythematosus--still current clinical and diagnostic problem].

Systemic lupus erythematosus (SLE) is a common autoimmune disease of unknown etiology that frequently affects women of childbearing age. SLE is associated with impairments in immune system, which is a consequence of increased production of various autoantibodies. This disease is still a major diagnostic and therapeutic problem.

Imbalance in cytokines from interleukin-1 family - role in pathogenesis of endometriosis.

Problem: To assess whether interleukin (IL)-1beta, IL-18 and interleukin-1 converting enzyme (ICE) are involved in the pathogenesis of endometriosis.

Method Of Study: Peritoneal fluid (PF) was obtained from 85 women with and without endometriosis. Peritoneal macrophages were cultured and the culture media collected.

[Changes in calprotectin concentration--inflammation marker in serum of women with gynecological cancer].

Objectives: Malignant tumors of the ovary and uterus remain to be a diagnostic and therapeutic problem in Poland, mainly due to the lack of effective diagnosis of their early stages. There is a relation between an impaired immune system, especially the process of inflammation and the pathogenesis of these tumors. The aim of the study was to assess the concentration of calprotectin--a inflammation marker in the serum of women with ovarian or uterine cancer.

Role of natural killer cell activity in the pathogenesis of endometriosis.

Natural Killer (NK) cells are cytotoxic effector lymphocytes with the ability to lyse target cells in a major histocompatibility complex (MHC) class Ι-independent manner and without the need for prior antigen exposure. Data strongly suggested that NK cells play an important role in human reproduction and disturbance in their function can favor development of the gynecological disorders. In our study the role of NK cells in pathogenesis of endometriosis is reviewed and summarized from available literature.

[Concentration of soluble ligand for receptor CD30 (sCD30L)--marker of apoptosis in women with ovarian tumor].

Objectives: Impairments of apoptosis processes are the basis of pathogenesis of many diseases, including ovarian tumors. The aim of the study was to evaluated the concentration of soluble ligand for receptor CD30 (sCD30L)--marker of apoptosis in women with ovarian tumor.

Material And Methods: The study comprised 80 women, aged from 21 to 62, and included 30 patients with Cystadenocarcinoma serosum la, 35 with Cystadenoma serosum and 15 women with Teratoma maturum.

[Concetration of anticardiolipin antybodies in peritonel fluid and in fluid from lymphocytes culture in women with endometriosis].

Aim: The aim of our work was to study both the concentration of anticardiolipin antibodies (aCL) in peritoneal fluid in women with endometriosis and to examine peritoneal lymphocyte ability to produce anticardiolipin antibodies.

Material And Methods: Study group included 30 women with endometriosis. The clinical stages of the disease were assessed by the revised American Fertility Society (rAFS) classification.

[Concentration of soluble Fas receptor (sFas) in serum of patients with ST-segment elevation myocardial infarction].

Introduction: Impairments ofapoptosis process, called programmed cell death, are the basis of pathogenesis of cardiovascular disease, including myocardial infarction. The aim of our study was to evaluated concentration of soluble receptor Fas (sFas)--marker ofapoptosis--in serum of patients with ST-segment elevation myocardial infarction.

Material And Methods: The study group consisted of 70 patients aged 42 to 75 (mean 58.

[Monocyte activity after stimulation by serum of women with endometriosis].

Objectives: Impairments of immune system play an important role in the development and pathogenesis of endometriosis. In affected women peritoneal macrophages are excessively activated, which is visible in an increased synthesis and release of macrophage-derived cytokines and growth factors. Monocytes are precursors of those cells in peripheral blood.

Levels of complement components iC3b, C3c, C4, and SC5b-9 in peritoneal fluid and serum of infertile women with endometriosis.

Objective: To assess whether complement components iC3b, C3c, C4, and SC5b-9 may be involved in the pathogenesis of endometriosis.

Design: Prospective, experimental trial.

Setting: Medical university.