Methyl transfer reactions catalyzed by cobalamin-dependent enzymes: Insight from molecular docking.

Methyl transfer reactions, mediated by methyltransferases (MeTrs), such as methionine synthase (MetH) or monomethylamine: CoM (MtmBC), constitute one of the most important classes of vitamin B-dependent reactions. The challenge in exploring the catalytic function of MeTrs is related to their modular structure. From the crystallographic point of view, the structure of each subunit has been determined, but there is a lack of understanding of how each subunit interacts with each other.

Laccase Enzyme Polymerization by Soft Plasma Jet for Durable Bioactive Coatings.

Conventional pin-to-point continuous wave Helium Corona plasma discharge was successfully used in Soft Plasma Polymerization (SPP) processes to immobilize into water and onto glass polymerized bioactive laccase coatings. The coatings were tested for bioactivity and durability under water wash. The coatings showed up to 59% bioactivity relative to the native laccase in water deposition, undoubtedly due to damage to and fragmentation of monomer molecules by the active, energetic species in the plasma.

Cold Plasma as an Innovative Construction Method of Voltammetric Biosensor Based on Laccase.

Development of new, faster methods of biosensor construction is a huge challenge for current science and industry. In this work, biosensor construction was carried out using a new soft plasma polymerization (SPP) method in which a bio-recognition layer of laccase enzyme was polymerized and bonded to a glassy carbon electrode (GCE) substrate under atmospheric pressure with a corona discharge jet. Laccase belongs to the oxidoreductase enzyme group with four copper atoms in its active center.

Theoretical predictions of anti-corrosive properties of THAM and its derivatives.

We present quantum chemical theoretical estimations of the anti-corrosive properties of THAM (tris(hydroxymethyl)aminomethane) and three derivatives that differ in the number of benzene rings: THAM-1 (2-amino-3-hydroxy-2-(hydroxymethyl) propylobenzoate), THAM-2 (2-amino-2-(hydroxymetyl)prapan-1,3-diyldibenzoate) and THAM-3 (2-amino-propan-1,2,3-triyltribenzoate). Fourteen exchange-correlation functionals based on the density functional theory (DFT) were chosen for quantum chemical study of THAM derivatives. The objective was to examine the effect of benzene rings on potential anti-corrosive properties of THAM compounds.

Structural and electronic properties of an [(Al2O3)4](+) cluster.

Density functional theory (DFT) has been applied to investigate the structural and electronic properties of an [(Al2O3)4](+) cluster. Since there is no structural data available from experiment, the geometry of the cluster was obtained based on a model which produced the best agreement with vibrational IR-MPD data. A range of different exchange-correlation functionals were tested, and it was concluded that the best spectral agreement was produced using the CAM-B3LYP and B3LYP functionals, respectively.