Publications by authors named "Justine Long"
With the increasing demand for alternative solutions to replace or optimize the use of synthetic fertilizers and pesticides, the inoculation of bacteria that can contribute to the growth and health of plants (PGPR) is essential. The properties classically sought in PGPR are the production of phytohormones and other growth-promoting molecules, and more rarely the production of exopolysaccharides. We compared the effect of two strains of exopolysaccharide-producing Rhizobium alamii on rapeseed grown in a calcareous silty-clay soil under water stress conditions or not.
View Article and Find Full Text PDFComplete Genome Sequences of Four Strains Isolated from Metal- and Radionuclide-Rich Soils.
Microbiol Resour Announc
October 2019
Here, we present the genome sequences of four strains, which were isolated at different locations in Europe from metal- or radionuclide-rich soils. High-quality complete genome sequences were obtained with PacBio and Illumina data sets with an original two-step procedure.
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- A9 cells were tested for the effects of 10 µM uranyl nitrate, comparing exposed bacteria to control groups over time intervals of 0.5, 4, and 24 hours.
- High-throughput proteomics analysis was conducted using advanced mass spectrometry to identify protein changes due to uranium exposure, utilizing a protein sequence database created from genome sequencing data.
- The findings contribute to a broader study on how a specific Chernobyl bacterial isolate responds to uranium stress, with data archived for further reference.
Milk and dairy products harbor a wide variety of bacterial species that compete for both limited resources and space. Under these competitive conditions, bacteria develop specialized mechanisms to protect themselves during niche colonization and nutrient acquisition processes. The bacterial antagonism mechanisms include the production of antimicrobial agents or molecules that facilitate competitor dispersal.
View Article and Find Full Text PDFUnlabelled: Microbacterium oleivorans A9 is a uranium-tolerant actinobacteria isolated from the trench T22 located near the Chernobyl nuclear power plant. This site is contaminated with different radionuclides including uranium. To observe the molecular changes at the proteome level occurring in this strain upon uranyl exposure and understand molecular mechanisms explaining its uranium tolerance, we established its draft genome and used this raw information to perform an in-depth proteogenomics study.
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- Desulfovibrio desulfuricans is a sulfate-reducing bacterium found in the human gut and the environment, capable of using nitrate and nitrite as electron acceptors to support its growth.
- The study combined experiments and data analysis to uncover how this bacterium responds to nitrate or nitrous oxide (NO), showing that its responses to these compounds are regulated independently despite their effects being interconnected.
- Key findings included the identification of various genes and transcription factors that manage the bacterium's metabolic responses, particularly highlighting a YtfE orthologue vital for repair from nitrosative damage.
Here, we present the draft genome sequence of strain A9, a uranium-tolerant actinobacterium which has been isolated from radionuclide-contaminated soil from the Chernobyl exclusion zone. It is composed of 22 contigs totaling 2,954,335 bp and contains 2,813 coding DNA sequences, one cluster of rRNA genes, and 45 tRNA genes.
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