Publications by authors named "Justine E Sunshine"
Article Synopsis
- A dengue virus vaccine targeting serotype 2 (DENV2) was developed using a protein and DNA approach, injected into rhesus macaques to assess its effectiveness.
- The EDIII-E2 vaccine triggered a strong immune response, with high levels of neutralizing antibodies detected after boosts, and protected vaccinated macaques from detectable viremia when exposed to the virus.
- The study determined that achieving a 50% neutralization titer of over 1:6000 before challenge was necessary for effective protection against DENV2, setting the stage for future vaccine development.
Unlabelled: To understand the interplay between host cytotoxic T-lymphocyte (CTL) responses and the mechanisms by which HIV-1 evades them, we studied viral evolutionary patterns associated with host CTL responses in six linked transmission pairs. HIV-1 sequences corresponding to full-length p17 and p24 gag were generated by 454 pyrosequencing for all pairs near the time of transmission, and seroconverting partners were followed for a median of 847 days postinfection. T-cell responses were screened by gamma interferon/interleukin-2 (IFN-γ/IL-2) FluoroSpot using autologous peptide sets reflecting any Gag variant present in at least 5% of sequence reads in the individual's viral population.
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- In vitro fitness assays are crucial for evaluating the replication fitness of viruses like HIV-1, using various metrics such as viral particle count and growth rate in cell cultures.
- Growth competition assays are highlighted as the most sensitive method for measuring fitness, focusing on how viruses compete for the same cellular resources under controlled conditions.
- The protocol outlined covers the process from creating a recombinant HIV-1 clone to conducting growth competition assays, emphasizing the importance of experimental parameters and providing flexibility in detection methods to accommodate different lab capabilities.*