Statistical Analysis of Photonic Crystal Spectra for the Independent Determination of the Size and Refractive Index of Cells.

Photonic crystal flow cytometry is a very attractive platform due to its great sensitivity in combination with a very compact design. Previous studies have demonstrated the possibility to use spectral processing for the measurement of a wide range of parameters, from simple object counting to independent analysis of the buffer solution and immersed microscale objects. Here we propose to go to the next level and simultaneously determine the shape and the refractive index of the cells.