Detection of malondialdehyde in vivo using microdialysis sampling with CE-fluorescence.

Oxidative damage is a naturally occurring process where reactive oxygen species (ROS) attack and disrupt normal cellular function; however, these effects become elevated during a stress event, such as ischemia/reperfusion or seizure. One result of oxidative stress is lipid peroxidation, where ROS attack free unsaturated fatty acids forming lipid hydorperoxides, which then break down to form secondary products acrolein, 4-hydroxynonenal, and malondialdehyde (MDA) resulting in irreversible membrane damage and ultimately cell death. Described here is a CE-fluorescence method for the determination of MDA in conjunction with in vivo microdialysis sampling.