Publications by authors named "Justas Ritmejeris"
Article Synopsis
- Peptide hormones undergo important post-translational modifications (PTMs) like tyrosine sulfation, crucial for functions such as plant growth.
- Detecting these modifications, particularly sulfotyrosine, faces challenges in isolation and measurement techniques.
- Nanopore technology can accurately identify and distinguish between different PTMs in single molecules, demonstrating over 90% accuracy in detecting sulfation and phosphorylation of peptide hormones.
Peptide phytohormones are decorated with post-translational modifications (PTMs) that are crucial for receptor recognition. Tyrosine sulfation on these hormones is essential for plant growth and development1. Measuring the occurrence and position of sulfotyrosine is, however, compromised by major technical challenges during isolation and detection2.
View Article and Find Full Text PDFHigh-throughput single-cell analysis typically relies on the isolation of cells of interest in separate compartments for subsequent phenotypic or genotypic characterization. Using microfluidics, this is achieved by isolating individual cells in microdroplets or microwells. However, due to cell-to-cell variability in size, shape, and density, the cell capture efficiencies may vary significantly.
View Article and Find Full Text PDFArticle Synopsis
- Current methods for detecting protein modifications, like phosphates, struggle with measuring single molecules and distinguishing closely spaced sites.
- The study introduces a technique that uses nanopores to analyze individual immunopeptide sequences that have cancer-related phosphate variants.
- This method successfully differentiates between peptides with one or two closely spaced phosphate groups with 95% accuracy for each single molecule read.