Publications by authors named "Juseok Kim"
Article Synopsis
- The urogenital microbiota plays a vital role in urinary tract health and the development of urinary tract infections, but the urogenital archaeome (a community of archaea) is not well understood.
- A study analyzed urine samples from 373 asymptomatic Korean individuals, finding a variety of archaea, including Methanobacteriota, Thermoproteota, and Halobacteriota, with notable variability among participants.
- The mean archaeal presence was 0.89%, and the findings suggest that the urogenital archaeome shares some characteristics with other body sites, enhancing our understanding of its potential influence on urinary tract health.
Article Synopsis
- * Phylogenetic analysis indicated that CBA7301 is closely related to the species JCM 17102, sharing 96.2% similarity in 16S rRNA gene sequences.
- * CBA7301 is characterized as a Gram-negative, strictly anaerobic, rod-shaped bacterium with specific growth conditions and distinctive lipids and fatty acids, and it has been designated as a new species.
Article Synopsis
- Alcoholic liver disease (ALD) encompasses conditions like fatty liver and cirrhosis and is a significant global health issue; this study investigates the potential protective effects of lactic acid bacteria (LAB) against ALD.
- Mice fed an alcohol diet showed higher liver enzyme levels and fat accumulation, but those treated with LAB (from kimchi) had lower liver damage indicators and fewer fat vesicles, indicating protective effects.
- The study concludes that ingesting LAB can help mitigate the negative impacts of alcohol on the liver, inflammation, and gut health by improving gut microbiota balance and reducing liver injury.
Two novel halophilic archaeal strains, CBA1133 and CBA1134, were isolated from solar salt in South Korea. The 16S rRNA gene sequences of the isolates were identical to each other and were closely related to the genera Natronomonas (92.3-93.
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- * Phylogenetic analysis revealed CBA3104 is distinct within the Brachybacterium genus, while CBA3105 is closely related to Brachybacterium halotolerans, showing high sequence similarity but differing in certain genomic metrics such as ANI and dDDH values.
- * Both strains share similar fatty acid profiles and contain menaquinone-7
Lactic acid bacteria (LAB) in kimchi, a traditional Korean food, are major fermentative microorganisms affecting the quality, safety, and nutritional and organoleptic properties of the final product. In this study, we determined the role of three key LAB strains, Leuconostoc gelidum, Latilactobacillus sakei, Weissella koreensis originated from different raw ingredients during natural fermentation, as opposed to an axenic environment. Starter cultures were inoculated into food with wild indigenous microbial communities, and the dynamics of bacterial communities and metabolites were analyzed during fermentation.
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- Researchers isolated a new Gram-positive, rod-shaped, anaerobic bacterium, strain CBA3637, from the Geum River sediment, showing it thrives at 30°C, 0% salt, and a pH of 7.
- Phylogenetic analysis revealed its closest relative is Aminipila butyrica, with about 96.6-96.7% similarity in 16S rRNA gene sequence.
- The study proposes that strain CBA3637 represents a new species, named Aminipila terrae, characterized by unique metabolic pathways and specific fatty acid profiles.
Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment.
Antonie Van Leeuwenhoek
May 2021
Article Synopsis
- - Strain CBA3638, isolated from the sediment of the Geum River in South Korea, is a Gram-positive, strictly anaerobic, rod-shaped bacterium optimal for growth at 37 °C, pH 7.0, and in 1.0% NaCl.
- - Phylogenetic analysis identifies CBA3638 as part of the genus Anaerocolumna in the Lachnospiraceae family, closely related to Anaerocolumna cellulosilytica, with a 15% DNA-DNA hybridization value indicating low genetic relatedness.
- - The genome of strain CBA3638, which is approximately 5.5 million base pairs long and features multiple rRNA
Article Synopsis
- Archaea, particularly methanogens, are under-researched in the human gut microbiota, with limited knowledge on their diversity and abundance.
- A study involving 897 East Asian participants in South Korea found that 42.47% of samples showed archaeal colonization, primarily dominated by haloarchaea, which accounted for an average of 10.24% of total microbial abundance.
- The study reveals significant variability in the human gut archaeome, categorizing it into four distinct archaeal enterotypes and highlighting its indigenous, responsive, and functional characteristics.
Article Synopsis
- A new strain of actinobacterium, designated CBA3103, was discovered in sediment from South Korea's Geum River and shows genetic similarities to LZ-22 with 98.47% similarity in 16S rRNA gene sequences.
- *The strain has specific characteristics: it is Gram-positive, rod-shaped, and thrives best at temperatures of 35°C, pH 7, and low salt concentrations.
- *Genomic analysis and chemotaxonomy indicate that CBA3103 is a unique species within its genus, proposed to be named sp. nov., with various distinct cellular components and growth conditions.*
Fermented foods constitute hubs of microbial consortia differentially affecting nutritional and organoleptic properties, quality, and safety. Here we show the origin source of fermentative microbes and fermentation dynamics of kimchi. We partitioned microbiota by raw ingredient (kimchi cabbage, garlic, ginger, and red pepper) to render kimchi fermented by each source-originated microbe pool and applied multi-omics (metataxonomics and metabolomics), bacterial viability, and physiochemical analyses to longitudinally collected samples.
View Article and Find Full Text PDFA Gram-stain-negative and facultatively aerobic bacterium, designated as strain CBA4604, was isolated from a traditional Korean salted and fermented shrimp food (saeu-jeot). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBA4604 formed a clearly distinct phyletic lineage from closely related species within the genus Paracoccus. Strain CBA4604 was the most closely related to P.
View Article and Find Full Text PDFWhite colony-forming yeasts (WCFYs) often appear in fermented foods, depending on the storage method. Despite the ongoing research on fermented foods, the community and genome features of WCFYs have not been well studied. In this study, the community structures of WCFYs on fermented vegetables (kimchi) prepared with various raw materials were investigated using deep sequencing.
View Article and Find Full Text PDFEnvironmental temperature is one of the most important factors for the growth and survival of microorganisms. Here we describe a novel extremely halophilic archaeon (haloarchaea) designated as strain CBA1119 isolated from solar salt. Strain CBA1119 had the highest maximum and optimal growth temperatures (66 °C and 55 °C, respectively) and one of the largest genome sizes among haloarchaea (5.
View Article and Find Full Text PDFPurpose: Bone marrow biopsy is a standard method for the evaluation of bone marrow infiltration by lymphoma; however, it is an invasive and painful procedure. Fluorodeoxyglucose positron emission tomography-computed tomography (FDG PET-CT) is a noninvasive imaging technique with the potential to detect bone marrow involvement by lymphoma.
Materials And Methods: We retrospectively reviewed medical records of lymphoma patients.
Although dextrocardia occurs rarely, the incidence of coronary artery disease is similar to the general population. Because of unfamiliarity with performing catheterization, transradial coronary angiography has seldom been performed in a patient with dextrocardia. We successfully performed left transradial coronary angiography in a patient with a right side heart using counter-directional torquing of the catheters and mirror-image angiographic angles.
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