Targeted regulation of 5-aminolevulinic acid enhances flavonoids, anthocyanins and proanthocyanidins accumulation in Vitis davidii callus.

Background: Spine grape (Vitis davidii) is a promising source of high-quality anthocyanins, with vast potential for application in food, pharmaceutical, and cosmetic industries. However, their availability is limited by resource constraints. Plant cell culture has emerged as a valuable approach for anthocyanin production and serves as an ideal model to investigate the regulation of anthocyanin biosynthesis.

Influences of PR06 Fermentation on Texture, Cooking Quality and Starch Digestibility of Oolong Tea-Fortified Rice Noodles.

is increasingly employed in food processing owing to its health benefits. Our previous research developed Oolong tea-fortified rice noodles with unique flavor and potent antioxidant activity; however, their texture still requires improvement. In this study, Oolong tea-fortified rice noodles were fermented using PR06 at inoculation amounts of 1%, 3%, and 5% (/), and assessed for cooking quality, texture, and starch digestibility.

Phytochemical and Flavor Characteristics of Mulberry Juice Fermented with BXM2.

Fermentation of mulberry juice not only improves its shelf life, but also effectively enhances their flavor and nutritional quality. This study elucidated the phytochemical and flavor characteristics of mulberry juice fermented with BXM2, originally isolated from naturally fermented fruit beverage, through widely targeted metabolomics. The fermentation produced the unique flavor of fermented juice and decreased the pH from 4.

N6-methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16-infected cervical squamous cell carcinoma.

Human papillomavirus (HPV) infection has been reported to be associated with N6-methyladenosine (m6A) modification in cancers. However, the underlying mechanism by which m6A methylation participates in HPV-related cervical squamous cell carcinoma (CSCC) remains largely unclear. In this study, we observed that m6A regulators methyltransferase like protein (METTL14) and insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) were upregulated in HPV-positive CSCC tissues and cell lines, and their high expression predicted poor prognosis for HPV-infected CSCC patients.

Curcumin-Loaded Bamboo Shoot Cellulose Nanofibers: Characterization and In Vitro Studies.

Given its high biological and pharmacological activities, curcumin (CUR) offers promising applications in functional foods. However, its low stability and bioavailability have greatly hindered its application in the food industry. The present study prepared cellulose nanofiber (CNF) from bamboo shoot processing byproducts and investigated its potential as a low-cost carrier.

Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages.

A soluble soybean polysaccharide SSPS1 with a molecular weight of 2737 kDa was extracted and purified from soy whey. SSPS1 was composed of glucose (97.3 %) and a small amount of mannose (2.

Bamboo shavings derived O-acetylated xylan alleviates loperamide-induced constipation in mice.

BSH-1 is an O-acetylated xylan obtained from bamboo shavings. This study determined the protective effects of BSH-1 against loperamide (Lop)-induced constipation in mice. Mice received BSH-1 by gavage daily for 14 days.

Selenium enrichment improves anti-proliferative effect of oolong tea extract on human hepatoma HuH-7 cells.

Selenium (Se)-enriched tea is attracting increasing interests due to its significantly improved health benefits. This study was to investigate the anti-proliferative effects of Se-enriched oolong tea against human hepatoma HuH-7 cells. Compared with regular oolong tea extract (TE, 0.

Pediococcus pentosaceus B49 from human colostrum ameliorates constipation in mice.

Constipation is a prevalent and burdensome gastrointestinal (GI) disorder that seriously affects the quality of human life. This study evaluated the effects of the P. pentosaceus B49 (from human colostrum) on loperamide (Lop)-induced constipation in mice.

Hypoglycemic effect of dietary fibers from bamboo shoot shell: An in vitro and in vivo study.

Bamboo shoot shell (BSS) was enzymatically decomposed to yield insoluble dietary fiber (IDF), soluble dietary fiber (SDF) and total dietary fiber (TDF), which were investigated for their hypoglycemic properties using in vitro and in vivo methods. The results indicated that SDF exhibited significantly higher glucose adsorbing capacity than those of IDF and TDF. Moreover, SDF showed similar inhibition potential against α-amylase with acarbose.

Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages.

Inflammatory response remains one of the most common and serious complications of disease. In order to profound understanding relationship between Phellinus linteus polysaccharides (TCM) and inflammation, inflammatory cell model was constructed by LPS acting RAW264.7 cell line.

Functional characterization of a potent anti-tumor polysaccharide in a mouse model of gastric cancer.

Aims: Natural polysaccharides are emerging as a new class of immunomodulatory agents due to their potent immunostimulatory effects and suitable biocompatibility. The aim of this study was to identify potent and selective anticancer activity of a bioactive polysaccharide.

Materials And Methods: In vitro, viability assay was performed to screen 16 of bioactive polysaccharides in a panel of normal and cancer cell lines.

In vitro fermentation of O‑acetyl‑arabinoxylan from bamboo shavings by human colonic microbiota.

BSH-1 is an O‑acetyl-arabinoxylan obtained from bamboo shavings. This study investigated its fermentation behavior by human colonic microbiota in vitro. Results showed that BSH-1 remarkably modulated the composition of human colonic microbiota, mainly by increasing the growth of potential beneficial genera (i.

Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13.

Bacillus subtilis strain SB13 which is isolated in our previous work was confirmed to produce endoglucanase. In this study, a novel endoglucanase gene (accession number: KX576676) was identified and cloned from SB13. Compared with other consensus sequence of reported endoglucanase genes in the GenBank database, this gene displays five differences (including T740C,A874G,A983G, T1210G and T1301C), which leading to five amino acid changes.

Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.

Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.

Isolation, chemical characterization, and immunomodulatory activity of naturally acetylated hemicelluloses from bamboo shavings.

Bamboo shavings, the outer or intermediate layer of bamboo stems, are the bulk of by-products produced in bamboo processing. In this study we investigated the isolation, chemical characterization, and immunostimulatory activity in vitro of the hemicelluloses from bamboo shavings. Shavings were first pretreated by steam explosion.