Publications by authors named "Junyi Wangxiao"

Article Synopsis
  • Duck/fish polyculture farming in southern China was studied for antibiotic resistance and metal levels by analyzing soil, water, and sediment samples from three farms in Guangdong Province in 2014.
  • The study found high prevalence of antibiotic resistance genes, particularly sul1, sul2, tetA, and floR, with significant correlations between the levels of copper and zinc and various resistance genes.
  • Certain bacteria appeared more frequently in the samples, with correlations indicating potential indicators of antibiotic resistance, highlighting the need to trace the origins of these genes for effective environmental protection strategies in aquaculture.
View Article and Find Full Text PDF

In this study, comet assay (single-cell gel electrophoresis), real-time quantitative PCR (qPCR) and proteomics approach were used to comprehensively assess toxicity elicited by roxarsone exposure in C. auratus at 50, 150 and 300 μg/L for 7, 14 and 21 days. Results of comet assay showed that DNA were seriously damaged under the pressure of roxarsone, especially the concentration of 50 μg/L that always maintained a sustained and increased damage effect to fish liver cell during the 21 days experiment.

View Article and Find Full Text PDF

The aim of this study was to investigate the fate of nine potential indicator antimicrobial resistance genes (ARGs) (sul1, sul2, tetB, tetM, ermB, ermF, fexA, cfr, intI1) and the diversity of bacterial communities in response to poultry manure applications to arable soil over a 90 day period. Quantitative real time PCR and Illumina high-throughput sequencing of 16S rDNA gene were used to quantify and trace ARG fate. The levels of all genes dramatically decreased over time and intI1, sul1, sul2 and tetM always had the greatest abundance and lowest dissipation rates.

View Article and Find Full Text PDF