Effects of spraying cycocel and delaying nitrogen application on lodging resistance and yield formation of winter wheat.

We conducted a 3-year (2017-2020) field experiment in the wheat base of Jinzhong Agricultural Hi-tech Industries Demonstration Zone, aiming to determine the measures of nitrogen topdressing and the regulatory effect of cycocel in spring to increase wheat yield. Four nitrogen topdressing dates were set up under the condition of cycocel spraying and control (CK) during the rising period: 10 days (D10), 20 days (D20), 30 days (D30), and 40 days (D40) after regreening stage, to analyze the impact of different N topdressing dates on winter wheat yield and the regulation effect of cycocel on stem characteristics, lignin content and related synthetase activities. The results showed that compared to other nitrogen topdressing dates, D30 increased spike number by 1.

The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat.

Regulation of grain size is a crucial strategy for improving the crop yield and is also a fundamental aspect of developmental biology. However, the underlying molecular mechanisms governing grain development in wheat remain largely unknown. In this study, we identified a wheat atypical basic helix-loop-helix (bHLH) transcription factor, TabHLH489, which is tightly associated with grain length through genome-wide association study and map-based cloning.

Identifying -Acting Elements Associated with the High Activity and Endosperm Specificity of the Promoters of Genes Encoding Low-Molecular-Weight Glutenin Subunits in Common Wheat ().

Low-molecular-weight glutenin subunits (LMW-GSs) associated with bread-baking quality and flour nutrient quality accumulate in endosperms of common wheat and related species. However, the mechanism underlying the expression regulation of genes encoding LMW-GSs has not been fully elucidated. In this study, we identified and , which are highly and weakly expressed, respectively, via the analysis of RNA-sequencing data of Chinese Spring wheat and wheat transgenic lines transformed with 5' deletion promoter fragments and fusion constructs.

Fine Mapping of the Wheat Leaf Rust Resistance Gene () and Validation of Its Co-segregation Markers.

Wheat leaf rust, caused by the fungus Eriks. (), is a destructive disease found throughout common wheat production areas worldwide. At its adult stage, wheat cultivar Liaochun10 is resistant to leaf rust and the gene for that resistance has been mapped on chromosome 2BS.

Interaction between serine carboxypeptidase-like protein TtGS5 and Annexin D1 in developing seeds of Triticum timopheevi.

GS5 encoding a serine carboxypeptidase-like protein positively regulates grain size and weight through the regulation of grain width and filling and is helpful in improving cereal yields. Grain width variation determined by GS5 is associated with cell number and size, but the actual underlying mechanism is still unclear. Two orthologs of GS5, TtGS5-3A-G and TtGS5-3G-G, were cloned from the Triticum timopheevi accession no.

Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity.

The specific and high-level expression of 1Ax1 is determined by different promoter regions. HMW-GS synthesis occurs in aleurone layer cells. Heterologous proteins can be stored in protein bodies.

Genome-wide identification of internal reference genes for normalization of gene expression values during endosperm development in wheat.

Internal reference genes that are stably expressed are essential for normalization in comparative expression analyses. However, gene expression varies significantly among species, organisms, tissues, developmental stages, stresses, and treatments. Therefore, identification of stably expressed reference genes in developmental endosperm of bread wheat is important for expression analysis of endosperm genes.

Effects of high-molecular-weight glutenin subunit combination in common wheat on the quality of crumb structure.

Background: High-molecular-weight glutenin subunits (HMW-GSs) have important effects on bread-making quality. Allelic variations of HMW-GS in bread wheat varieties contribute in different ways to dough properties and bread volume. However, no systematic analysis has been done on the effects of allelic variation on bread-crumb structure, an important parameter when evaluating bread-making quality.