Integrated Transcriptome and Metabolome Analysis Reveals the Resistance Mechanisms of Against .

Rapeseed ( L.) is an important crop for healthy edible oil and stockfeed worldwide. However, its growth and yield are severely hampered by black rot, a destructive disease caused by pv.

Structural variation reshapes population gene expression and trait variation in 2,105 Brassica napus accessions.

Although individual genomic structural variants (SVs) are known to influence gene expression and trait variation, the extent and scale of SV impact across a species remain unknown. In the present study, we constructed a reference library of 334,461 SVs from genome assemblies of 16 representative morphotypes of neopolyploid Brassica napus accessions and detected 258,865 SVs in 2,105 resequenced genomes. Coupling with 5 tissue population transcriptomes, we uncovered 285,976 SV-expression quantitative trait loci (eQTLs) that associate with altered expression of 73,580 genes.

Incidence and prognosis of acute kidney injury versus acute kidney disease among 71 041 inpatients.

Background: Acute kidney disease (AKD) defines patients with acute kidney injury (AKI) or subacute loss of kidney function lasting for >7 days. Little is known about the prognosis of AKD in hospitalized patients. The aim of this study was to investigate the risk factors and prognosis of AKD and to compare different types of acute/subacute renal impairment among Chinese inpatients.

Pathotype Characterization of by European Clubroot Differential and Williams Sets in China.

Clubroot disease caused by the soil-borne is devastating to Brassicaceae crops and spreading rapidly in China in recent years, resulting in great yield losses annually. Virulence of populations specializes and is in dynamic change in the fields. Information on the pathotypes and their distributions is crucial to control the clubroot disease.

Hepatocellular carcinoma cell differentiation trajectory predicts immunotherapy, potential therapeutic drugs, and prognosis of patients.

The aim of this study is to explore a novel classification and investigate the clinical significance of hepatocellular carcinoma (HCC) cells. We analyzed integrated single-cell RNA sequencing and bulk RNA-seq data obtained from HCC samples. Cell trajectory analysis divided HCC cells into three subgroups with different differentiation states: state 1 was closely related to phosphoric ester hydrolase activity, state 2 was involved in eukaryotic initiation factor 4E binding, translation regulator activity and ribosome, and state 3 was associated with oxidoreductase activity and metabolism.

Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.

Background: Oilseed rape (Brassica napus L.) is known as one of the most important oilseed crops cultivated around the world. However, its production continuously faces a huge challenge of Sclerotinia stem rot (SSR), a destructive disease caused by the fungus Sclerotinia sclerotiorum, resulting in huge yield loss annually.

Integrating GWAS, linkage mapping and gene expression analyses reveal the genetic control of first branch height in L.

Rapeseed ( L.) is a crucial oil crop cultivated worldwide. First branch height, an essential component of rapeseed plant architecture, has an important effect on yield and mechanized harvesting; however, the underlying genetic mechanism remains unclear.

Construction of Diverse -Heterocycles by Formal (3 + 3) Cycloaddition of Naphthol/Thionaphthol/Naphthylamine and 1,3,5-Triazinanes.

Here, we report a facile and metal-free method for the construction of dihydrooxazine derivatives via a formal (3 + 3) annulation reaction of naphthols and 1,3,5-triazinanes. The 1,3,5-triazinanes were utilized as a formal three-atom synthon (C-N-C) for cycloaddition. In addition, dihydrothiazine and tetrahydrobenzoquinazoline derivatives could also be produced in good yields by this strategy under catalyst-free and additive-free conditions.

Alternative splicing reprogramming in fungal pathogen at different infection stages on .

Alternative splicing (AS) is an important post-transcriptional mechanism promoting the diversity of transcripts and proteins to regulate various life processes in eukaryotes. Sclerotinia stem rot is a major disease of caused by Sclerotinia sclerotiorum, which causes severe yield loss in production worldwide. Although many transcriptome studies have been carried out on the growth, development, and infection of S.

The plant trans-Golgi network component ECHIDNA regulates defense, cell death, and endoplasmic reticulum stress.

The trans-Golgi network (TGN) acts as a central platform for sorting and secreting various cargoes to the cell surface, thus being essential for the full execution of plant immunity. However, the fine-tuned regulation of TGN components in plant defense and stress response has been not fully elucidated. Our study revealed that despite largely compromising penetration resistance, the loss-of-function mutation of the TGN component protein ECHIDNA (ECH) induced enhanced postinvasion resistance to powdery mildew in Arabidopsis thaliana.

Analysis of Tissue-Specific Defense Responses to in .

Sclerotinia stem rot (SSR) caused by () is the main disease threat of oilseed rape (), resulting in huge economic losses every year. SSR resistance manifests as quantitative disease resistance (QDR), and no gene with complete SSR resistance has been cloned or reported so far. Transcriptome analysis has revealed a large number of defense-related genes and response processes.

Genome-Wide Characterization of Serine/Arginine-Rich Gene Family and Its Genetic Effects on Agronomic Traits of .

Serine/arginine-rich (SR) proteins are indispensable factors for RNA splicing, and they play important roles in development and abiotic stress responses. However, little information on genes in is available. In this study, 59 genes were identified and classified into seven subfamilies: SR, SCL, RS2Z, RSZ, RS, SR45, and SC.

Mapping of a major QTL controlling plant height using a high-density genetic map and QTL-seq methods based on whole-genome resequencing in Brassica napus.

Plant height is a crucial element related to plant architecture that influences the seed yield of oilseed rape (Brassica napus L.). In this study, we isolated a natural B.

Comparing the Infection Biology of in Clubroot Susceptible and Resistant Hosts and Non-hosts.

The potential infection biology of in resistant hosts and non-hosts is still not completely understood. Clubroot resistance assay on European clubroot differentials (ECD) set revealed that ECD10 () and ECD4 () show a complete resistance to the tested isolate in contrast to highly susceptible hosts Westar () and ECD5 (). Previously, we used fluorescent probe-based confocal microscopy (FCM) to refine the life cycle of and indicate the important time points during its infection in .

Physicians' attitudes towards reproduction in young patients with early breast cancer in China.

Background: As more young patients with breast cancer undergo treatments and obtain good prognoses, the issue of postoperative reproduction in breast cancer patients has attracted more attention.

Methods: We conducted a prospective, cross-sectional survey of 2000 breast cancer-associated physicians using a 24-items questionnaire adapted from prior guides. Then we used a multivariable linear regression model to confirm independent associations between the propensity of physicians' attitudes toward reproduction and physicians' specific demographic characteristics.

Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury.

Background: Krüppel-like factor 6 (KLF6) is a transcription factor that participate in various pathophysiological processes, but its contribution in ischemia acute kidney injury (AKI) is lacking so far. The study aimed to investigate the expression and the role of KLF6 in kidney ischemia-reperfusion (IR) injury.

Method: Microarray data were collected from GSE58438 and GSE52004.

The Arabidopsis CCCH protein C3H14 contributes to basal defense against Botrytis cinerea mainly through the WRKY33-dependent pathway.

Necrotrophic pathogens such as Botrytis cinerea cause significant crop yield losses. Plant CCCH proteins play important roles in pathogen resistance responses. However, the CCCH-mediated defense mechanisms against necrotrophic pathogens are unclear.

A global survey of the transcriptome of allopolyploid Brassica napus based on single-molecule long-read isoform sequencing and Illumina-based RNA sequencing data.

Brassica napus is a recent allopolyploid derived from the hybridization of Brassica rapa (A A ) and Brassica oleracea (C C ). Because of the high sequence similarity between the A and C subgenomes, it is difficult to provide an accurate landscape of the whole transcriptome of B. napus.

Identification of Flower-Specific Promoters through Comparative Transcriptome Analysis in .

(oilseed rape) is an economically important oil crop worldwide. Sclerotinia stem rot (SSR) caused by is a threat to oilseed rape production. Because the flower petals play pivotal roles in the SSR disease cycle, it is useful to express the resistance-related genes specifically in flowers to hinder further infection with .