Publications by authors named "Junxiao Dai"
Ion mobility mass spectrometry (IM-MS) measures the mass, size, and shape of ions in the same experiment, and structural information is provided via collision cross-section (CCS) values. The majority of commercially available IM-MS instrumentation relies on the use of CCS calibrants, and here, we present data from a family of poly(l-lysine) dendrimers and explore their suitability for this purpose. In order to test these compounds, we employed three different IM-MS platforms (Agilent 6560 IM-QToF, Waters Synapt G2, and a home-built variable temperature drift tube IM-MS) and used them to investigate six different generations of dendrimers in two buffer gases (helium and nitrogen).
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- The study focuses on the SARS-CoV-2 nucleocapsid protein (Ncap), which is important for diagnostics and vaccine development since the Covid-19 pandemic started in 2019.
- Researchers successfully expressed and purified SARS-CoV-2 Ncap proteins in E. coli, specifically using a synthetic gene with a 6His affinity tag to enhance purification.
- ELISA assays were conducted to compare the immune response of human sera to different Ncap proteins, showing no major differences between tagged and untagged versions, except a slight potential decrease in sensitivity to some Ncap variants.