Metagenomic analysis revealed the bioremediation mechanism of lead and cadmium contamination by modified biochar synergized with PSB-2 in phosphate mining wasteland.

Introduction: Phosphate mining wasteland is contaminated with heavy metals, such as lead (Pb) and cadmium (Cd), which pose significant environmental risks. Ecological restoration of these lands is crucial, but limited research has focused on the remediation of heavy metal-contaminated soils using modified biochar and functional microorganisms.

Methods: In this study, we investigated the bioremediation of phosphate mining wasteland soil using modified biochar in combination with the phosphate-solubilizing bacterium .

Enhanced Pb immobilization by CaO/MgO-modified soybean residue (okara) in phosphate mining wasteland soil: Mechanism and microbial community structure.

Lead (Pb) contamination is an inevitable consequence of phosphate mining, necessitating the development of effective remediation strategies. This study investigated the use of CaO/MgO-modified okara (CMS) as an eco-friendly approach to remediate Pb-contaminated soils from phosphate mining wastelands. In the present study, following 30 d of CMS application, the exchangeable Pb content was significantly decreased to 10.

Heterotrophic nitrification processes driven by glucose and sodium acetate: New insights into microbial communities, functional genes and nitrogen metabolism from metagenomics and metabolomics.

Heterotrophic nitrification (HN) bacteria use organic carbon sources to remove ammonia nitrogen (NH-N); however, the mechanisms of carbon and nitrogen metabolism are unknown. To understand this mechanism, HN functional microbial communities named MG and MA were enriched with glucose and sodium acetate, respectively. The NH-N removal efficiencies were 98.

Highly efficient recovery of phosphate and fluoride from phosphogypsum leachate: Selective precipitation and adsorption.

Phosphogypsum, a typical by-product in the phosphorus chemical industry, could generate a large amount of leachate containing phosphate and fluoride in the process of rainfall and long-term stacking, which not only causes serious environmental pollution, but also leads to a waste of resources. In this study, a united treatment of calcium hydroxide precipitation and lanthanum zeolite (La-ZFA) adsorption was proposed to achieve the recovery of phosphate and fluoride from phosphogypsum leachate. In phosphogypsum, most phosphorus could be leached except P in the residual occurrence form, while for fluoride, only water-soluble F could be effectively leached.

Immunogenic cell death-based cancer vaccines: promising prospect in cancer therapy.

Tumor immunotherapy is a promising approach for addressing the limitations of conventional tumor treatments, such as chemotherapy and radiotherapy, which often have side effects and fail to prevent recurrence and metastasis. However, the effectiveness and sustainability of immune activation in tumor immunotherapy remain challenging. Tumor immunogenic cell death, characterized by the release of immunogenic substances, damage associated molecular patterns (DAMPs), and tumor associated antigens, from dying tumor cells (DTCs), offers a potential solution.

Molecular insights into effects of chemical conditioning on dissolved organic phosphorus transformation and bioavailability during sludge composting.

Phosphorus is enriched in waste activated sludge (WAS) during wastewater treatment, and organic phosphorus (OP) is a potential slow-release P fertilizer. The chemical coagulants used in sludge dewatering leave numerous residues in WAS that affect sludge composting. In this study, the effects of polyaluminum chloride (PAC) and polyferric sulfate (PFS) on the bioconversion of dissolved OP (DOP) during sludge composting were investigated.

Phosphorus immobilization/release behavior of lanthanum-modified bentonite amended sediment under the dual effects of pH and dissolved organic carbon.

Lanthanum modified bentonite (LMB) is typical P-inactivating agent that has been applied in over 200 lakes. Dissolved organic carbon (DOC) and high pH restrict the phosphorus (P) immobilization performance of LMB. However, the P immobilization/release behaviors of LMB-amended sediment when suspended to overlying water with high pH and DOC have not yet been studied.

Mechanistic investigation of Pb adsorption on biochar modified with sodium alginate composite zeolitic imidazolate framework-8.

For the serious situation of heavy metal pollution, the use of cheap, clean, and efficient biochar to immobilize heavy metals is a good treatment method. In this paper, SA@ZIF-8/BC was prepared for the adsorption of Pb in solution using sodium alginate (SA) and zeolitic imidazolate framework-8 (ZIF-8) modified corn cob biochar. The results showed that the specific surface area of modified biochar was greatly improved, with good adsorption capacity for Pb, strong anti-interference ability, and good economy.

Mechanistic insights into chemical conditioning on transformation of dissolved organic matter and plant biostimulants production during sludge aerobic composting.

Inorganic coagulants (aluminum and iron salt) are widely used to improve sludge dewaterability, resulting in numerous residues in dewatered sludge. Composting refers to the controlled microbial process that converts organic wastes into fertilizer, and coagulant residues in dewatered sludge can affect subsequent compost efficiency and resource recycling, which remains unclear. This work investigated the effects of two typical metal salt coagulants (poly aluminum chloride [PAC] and poly ferric sulfate [PFS]) conditioning on sludge compost.

Comparison of microbial communities in unleached and leached ionic rare earth mines.

The leaching of ionic rare earth elements has caused serious environmental pollution and ecological damage. Microorganisms play a crucial role in soil ecosystems and are one of the most important components of these systems. However, there are fewer studies related to the changes that occur in microbial community structure and diversity before and after leaching in ionic rare earth mines.

Inhibitory effect of microplastics derived organic matters on humification reaction of organics in sewage sludge under alkali-hydrothermal treatment.

Alkali-hydrothermal treatment (AHT) of sewage sludge is often used to recover value-added dissolved organic matters (DOM) enriched with artificial humic acids (HA). Microplastics (MPs), as emerging contaminants in sewage sludge, can leach organic compounds (MP-DOM) during AHT, which potentially impact the characteristics of thermally treated sludge's DOM. This study employed spectroscopy and Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR-MS) to explore the impacts of MPs on DOM composition and transformation during AHT.

Metagenomic analysis revealed the evolution of microbial communities, metabolic pathways, and functional genes in the heterotrophic nitrification-aerobic denitrification process under La stress.

Trivalent lanthanum (La) exists widely in ammonia nitrogen (NH-N) tailing water from ionic rare earth mines; however, its effect on heterotrophic nitrification-aerobic denitrification (HN-AD) is unknown, thereby limiting the application of the HN-AD process in this field. In this study, we conducted an HN-AD process using a sequencing batch reactor (5 L) that was continuously operated to directly treat acidic (NH)SO wastewater (influent NH-N concentration of approximately 110 mg/L and influent pH of 5) containing different La concentrations (0-100 mg/L). The NH-N removal efficiency of the reactor reached 98.

Nanocarrier-Mediated Immunogenic Cell Death for Melanoma Treatment.

Melanoma, a highly aggressive skin tumor, exhibits notable features including heterogeneity, a high mutational load, and innate immune escape. Despite advancements in melanoma treatment, current immunotherapies fail to fully exploit the immune system's maximum potential. Activating immunogenic cell death (ICD) holds promise in enhancing tumor cell immunogenicity, stimulating immune amplification response, improving drug sensitivity, and eliminating tumors.

Double-edged effect of frequent freeze-thaw on the stability of zero-valent iron after heavy metal remediation.

Freeze-thaw cycles (FTCs) cause dynamic microscale changes in ions and solvents. During freezing, heavy metals adsorbed on zero-valent iron (M-ZVI) and protons are excluded by ice crystals and concentrated in the liquid-like grain boundary region. The high proton concentration in this region leads to the dissolution of the passivation layer of ZVI.

Synergistic leaching process for ion-exchange ammonium from weathered crust elution deposited rare earth tailings with potassium magnesium compound eluent.

The ion-exchangeable ammonium (IE-A) that accounts for 60-90% of the total residual ammonium in rare earth tailings has great potential to pollute the surrounding environment, and much research has been done to seek an effective elution method. However, the current study mainly focused on the single salt solution, which made it hard to reach the desired elution efficiency. In this study, the efficient binary compound eluent was prepared, and the response surface experiments and dynamic elution were performed to optimize the elution condition and evaluate the practical application prospect.

Molecular transformation pathway and bioavailability of organic phosphorus in sewage sludge under vermicomposting.

Phosphorus reclamation from sewage sludge is essential for sustainable phosphorus management, as large quantities of phosphorus afflux into wastewater treatment plants and are finally enriched in sewage sludge via phosphorus removal technologies. Meanwhile, vermicomposting is a cost-effective biotechnique for sludge stabilization. This work unveiled the molecular transformation pathway and bioavailability of organic phosphorus (OP) in sludge under vermicomposting with solution P NMR, FT-ICR MS and enzymatic hydrolysis assay.

Environmental impact and optimization suggestions of pig manure and wastewater treatment systems from a life cycle perspective.

With the rapid development of the pig farming industry in China, a large amount of pig manure is inevitably generated. The management and utilization of pig manure in a sustainable approach require a systematic analysis of the environmental impacts generated from the existing pig manure treatment and disposal routes. In this study, three typical pig manure treatment and disposal routes: pig manure solid-liquid separation, i) wastewater biological treatment, direct land application of manure/sludge; ii) black-film anaerobic digestion of wastewater, mono-composting of manure/sludge and land use; iii) wastewater biological treatment, co-composting of manure/sludge and land use, were comparatively assessed in terms of their environmental impacts using the life cycle assessment.

Mechanisms of combined bioremediation by phosphate-solubilizing fungus and plants and its effects on cadmium contamination in phosphate-mining wastelands.

Owing to uncontrolled mining activities and lack of ecological protection measures, phosphate-mining wastelands are contaminated with the heavy metal Cd. In this study, Penicillium oxalicum strain ZP6, a Cd-resistant phosphate-solubilizing fungus, was used in combination with the fast-growing, high-biomass plant Brassica juncea L. to enhance Cd remediation in phosphate-mining wastelands.

PPh/I Promoted Synthesis of Unsymmetrical Disulfides from Sodium Sulfites and 2-Mercaptobenzo Heterocyclics.

A simple and efficient method for the synthesis of unsymmetrical disulfides is reported. Using sodium sulfites and 2-mercaptobenzo heterocyclic compounds as starting materials, the unsymmetrical sulfur-sulfur bonds could be quickly constructed in the PPh/I reaction system under transition-metal-free conditions. This protocol has the advantages of mild reaction conditions, easily available starting materials, and wide substrate scope, showing potential synthetic value for the synthesis of a diversity of biologically or pharmaceutically active compounds.

Advance and Challenges in the Treatment of Skin Diseases with the Transdermal Drug Delivery System.

Skin diseases are among the most prevalent non-fatal conditions worldwide. The transdermal drug delivery system (TDDS) has emerged as a promising approach for treating skin diseases, owing to its numerous advantages such as high bioavailability, low systemic toxicity, and improved patient compliance. However, the effectiveness of the TDDS is hindered by several factors, including the barrier properties of the stratum corneum, the nature of the drug and carrier, and delivery conditions.

Treating waste with waste: Adsorption of anionic dyes in wastewater with surfactant-modified phosphogypsum.

Phosphogypsum (PG) is a solid waste generated during the wet process of phosphoric acid production. The environmental-friendly disposal and recycling of PG is vital in the field of environmental solid waste treatment. In this study, PG is used for adsorbent of dyes in wastewater to achieve the goal of recycling waste with waste.

Combining network pharmacology, molecular docking, molecular dynamics simulation, and experimental verification to examine the efficacy and immunoregulation mechanism of FHB granules on vitiligo.

Background: Fufang Honghua Buji (FHB) granules, have proven efficacy against vitiligo in long-term clinical practice. However, its major active chemical components and molecular mechanisms of action remain unknown. The purpose of this study was to confirm the molecular mechanism of FHB's therapeutic effect on vitiligo utilizing network pharmacology, molecular docking, and molecular dynamics simulation prediction, as well as experimental verification.

Performance changes in the anammox process under the stress of rare-earth element Ce(III) and the evolution of microbial community and functional genes.

The high Ce(III) content in ionic rare-earth tailings wastewater has hindered the application of anammox process in this field. Here, the effect of Ce(III) on the performance of anammox processes was investigated, and the evolution of microbial communities and functional genes was explored using metagenomic sequencing. The results showed that the reactor nitrogen removal rate decreased when the Ce(III) concentration reached 25 mg/L, although ammonia nitrogen removal (92.