Development of a landing pad system for Aspergillus niger and its application in the overproduction of monacolin J.

Aspergillus niger is a powerful and efficient cell factory, with the potential to synthesize valuable products as chassis cells. The use of microbial cell factories to produce monacolin J, a precursor for statin synthesis, as an alternative to chemical synthesis could meet increasing market demand. However, the need for precise large fragment gene editing and the availability of suitable integration loci hinders the application of this strain.

AcMYB96 promotes anthocyanin accumulation in onion (Allium cepa L) without forming the MBW complex.

Anthocyanins are major flavonoid compounds with established health benefits. Although the molecular mechanisms of MYB transcription factors (TFs) within the MYB-basic helix-loop-helix (bHLH)-WD-repeat protein (MBW) complex in anthocyanin biosynthesis have been revealed, the functions of other MYB TFs that are unable to form the MBW complex in this process remain unclear. In this study, we uncovered and extensively characterized an R2R3-MYB TF in onion (Allium cepa L.

Association between alcohol consumption and sleep traits: observational and mendelian randomization studies in the UK biobank.

Previous studies have shown that excessive alcohol consumption is associated with poor sleep. However, the health risks of light-to-moderate alcohol consumption in relation to sleep traits (e.g.

The combined effects of arteriosclerosis and diabetes on cardiovascular disease risk.

The morbidity and mortality of cardiovascular disease (CVD) rank first among common diseases. Arteriosclerosis and diabetes are risk factors for CVDs, which influence each other. However, their combined effects on CVDs are still unclear.

Circum-Psoas Block versus Supra-Inguinal Fascia Iliaca Block for Postoperative Analgesia in Patients Undergoing Total Hip Arthroplasty: A Randomized Clinical Trial.

Purpose: Total hip arthroplasty (THA) is often associated with moderate to severe pain. The present study compared the efficacy of circum-psoas block (CPB) with supra-inguinal fascia iliaca block (SIFIB) for postoperative analgesia in patients undergoing THA.

Patients And Methods: In this randomized trial, sixty-four patients undergoing THA were allocated randomly to the CPB group or SIFIB group with 40 mL of 0.

Characteristics of the knowledge graph of scientific and technological innovation in Gansu Province.

The knowledge graph based on research papers can accurately identify and present the latest developments in scientific and technological (S&T) innovation and is of great significance for supporting strategic decision-making relating to S&T innovation in undeveloped areas. Based on the international research papers produced in Gansu Province during the 13th Five-Year Plan period (2016-2020), five metrics, including the number and characteristics of papers, co-authors, main publications and their fields, major supporting institutions, and main research areas, are established herein. The results indicate that: (i) the total of 29,951 papers were published, which is about 2.

Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin in .

is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current expression system is the difference in expression yield of heterologous proteins of non-fungal origin compared to those of fungal origin, which is about three orders of magnitude. The sweet protein monellin, derived from West African plants, has the potential to become a food-grade sweetener due to its high sweetness and the benefit of not containing sugar itself, but it is extremely difficult to establish a research model for heterologous expression in , owing to extremely low expression, a small molecular weight, and being undetectable with conventional protein electrophoresis.

Full neurological recovery from severe nonexertional heat stroke with multiple organ dysfunction: A case report.

Background: We report a rare case of full neurological recovery from severe nonexertional heat stroke in a 67-year-old woman with an initial Glasgow Coma Scale of 3. This report raises awareness among doctors that when heatstroke is diagnosed, comprehensive treatment should be implemented as soon as possible. Moreover, targeted temperature management, combination therapy with hemodialysis and hemoperfusion, and hyperbaric oxygen therapy may alleviate multiorgan failure and prevent neurological sequelae caused by heatstroke.

receptor impacts mycelial growth its interactions with cell wall synthase and transporters in .

Efficient protein secretion is closely correlated with vesicle sorting and packaging, especially with cargo receptor-mediated selective transport for ER exit. Even though is considered an industrially natural host for protein production due to its exceptional secretion capacity, the trafficking mechanism in the early secretory pathway remains a black box for us to explore. Here, we identified and characterized all putative ER cargo receptors of the three families in We successfully constructed overexpression and deletion strains of each receptor and compared the colony morphology and protein secretion status of each strain.

[Appropriate Use and Abuse of Sedative-Hypnotic Drugs].

The incidence of insomnia has been increasing in recent years. In addition, due to the impact of the COVID-19 pandemic, more and more people are experiencing a variety of insomniac problems, including having difficulty in sleep initation, waking up too early, and short sleep duration. Chronic insomnia may seriously affect patients' life and work, increase their risks of developing physical and mental illnesses, and cause crushing social and economic burdens.

CoO embedded porous biomass-derived carbon as dual-functional host material for lithium-sulfur batteries.

A new strategy is developed to fabricate sulfur electrode by infusing sulfur into a conductive biochar decorated with highly dispersed CoO nanoparticles. The loading of the CoO nanoparticles, as the active sites for reactions, is efficiently increased by using the microwave-assisted diffusion method. It is demonstrated that biochar can serve as an excellent conductive framework to effectively activate sulfur.

Cobalt-free nickel-rich cathode materials based on Al/Mg co-doping of LiNiO for lithium ion battery.

To develop Co-free LiNiO-based layered cathode materials is crucial for meeting the demands of the lithium-ion batteries with high energy density, long cycling life, and low cost. Herein, the LiNiAlMgO materials are synthesized by the solid-solid interface elemental interdiffusion strategy. It is elucidated that the Mg and Al ions are mainly doped in the Li slabs and transition metal slabs, respectively, leading to the alteration of the crystal lattice.

Efficacy and tolerability of pharmacological treatments for insomnia in adults: A systematic review and network meta-analysis.

Insomnia is one of the most common and burdensome disorders in adults. We compared and ranked insomnia medication on the basis of their efficacy and tolerability. We performed a systematic review and network meta-analysis of placebo-controlled or head-to-head randomized controlled trials for primary insomnia in adults comparing 20 drugs.

Influences of (in)congruences in psychological entitlement and felt obligation on ethical behavior.

Introduction: Psychological entitlement and felt obligation are two correlated but distinctive conceptions. Prior studies have mainly explored their influences on employees' (un)ethical behavior, respectively. Recently, several studies suggest the interactive impacts of psychological entitlement with felt obligation on individual behavioral choices.

Understanding the role of peer pressure on engineering students' learning behavior: A TPB perspective.

Introduction: With the advent of the digital age, the gradually increasing demands of the engineering job market make it inevitable that engineering students face the pressures that arise from academic life with their peers. To address this issue, this study aims to explore the influence of engineering students' peer pressure on learning behavior based on the theory of planned behavior (TPB).

Methods: In addition to attitudes, subjective norms, and perceived behavioral controls inherent in TPB, two new dimensions-gender difference and peer academic ability-were incorporated to construct a framework of the dimensions of peer pressure as affecting engineering students as well as an expanded model of TPB.

The Depleting Impact of Helping Behavior on Career Satisfaction: The Buffering Role of Strengths Use.

Recent studies have explored the dark side of helping behavior from an actor-centric perspective. Consistent with this stream of research, this study linked helping behavior to career satisfaction. In this study, we adopted perceived task demands and job strain as two sequential mediators to elaborate the underlying depletion path through which helping behavior undermines career satisfaction.

Challenge or Hindrance? The Dual Path Effect of Perceived Task Demand on In-Role Performance and Work Fatigue.

The evidence for the existence of perceived task demand is paradoxical. The purpose of the present study is to explore whether perceived task demand is a challenge or a hindrance stressor. To achieve this research purpose, based on conservation of resources theory, a conceptual model is developed that utilizes both a resource acquisition path and a resource depletion path.

Being helpful and being innovative: The role of psychological meaningfulness and positive affect.

The present study is developed based on conservation of resources theory (COR) to explore the underlying mechanism and boundary condition for the relationship between helping behavior and innovative behavior. To avoid the shortages of cross-sectional data, the present study collected two-wave and multi-source data. By collecting from 193 full-time Chinese workers and 68 supervisors at two separate time points, this study developed and examined a moderated mediation model using Mplus 7.