Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (.).

Figs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection.

In vitro ATP regeneration via immobilized anaerobic glycolytic enzymes: System construction and application in l-theanine production.

Many biocatalytic reactions require ATP as an energy source, and the regeneration of ATP is crucial in enzyme-catalyzed reactions with high energy demands. In this study, an ATP regeneration system was constructed via immobilized anaerobic glycolytic enzymes coupled with γ-glutamylmethylamide synthase (GMAS), which aimed at synthesizing L-theanine through an in vitro pathway. Results showed that soluble expression of the histidine-tagged anaerobic glycolytic enzyme and GMAS was successfully achieved in Escherichia coli BL21 (DE3), with specific activities ranging from 2.

Functional Characterization of Promoter in Response to Hormones and Wounding Stress in Transgenic Tobacco.

is a model species for studying natural rubber biosynthesis because its root can produce high-quality rubber. Small rubber particle protein (SRPP), a stress-related gene to multiple stress responses, involves in natural rubber biosynthesis. To investigate the transcriptional regulation of the promoter, the full-length promoter PR0 (2188 bp) and its four deletion derivatives, PR1 (1592 bp), PR2 (1274 bp), PR3 (934 bp), and PR4 (450 bp), were fused to β-glucuronidase (GUS) reporter gene and transformed into tobacco.

Heteroatom-Doping of Non-Noble Metal-Based Catalysts for Electrocatalytic Hydrogen Evolution: An Electronic Structure Tuning Strategy.

Electrocatalytic water splitting for hydrogen production is an appealing way to reduce carbon emissions and generate renewable fuels. This promising process, however, is limited by its sluggish reaction kinetics and high-cost catalysts. Construction of low-cost and high-performance non-noble metal-based catalysts have been one of the most effective approaches to address these grand challenges.

Thermal Puffing Promoting the Synthesis of N-Doped Hierarchical Porous Carbon-CoO Composites for Alkaline Water Reduction.

N-doped porous carbon-based catalysts hold great promise for hydrogen evolution reaction (HER) due to their plentiful cavity construction, high specific surface area, and flexible metal assemblies. Nevertheless, the cumbersome synthetic process and the use of highly corrosive chemicals greatly increase the production costs and pollutions. Herein, we report a facile and eco-friendly thermal puffing strategy, which imitates the popcorn forming process, for the fabrication of N-doped hierarchical porous carbon-CoO catalysts.

Efficient Oxygen Reduction Reaction (ORR) Catalysts Based on Single Iron Atoms Dispersed on a Hierarchically Structured Porous Carbon Framework.

Single Fe atoms dispersed on hierarchically structured porous carbon (SA-Fe-HPC) frameworks are prepared by pyrolysis of unsubstituted phthalocyanine/iron phthalocyanine complexes confined within micropores of the porous carbon support. The single-atom Fe catalysts have a well-defined atomic dispersion of Fe atoms coordinated by N ligands on the 3D hierarchically porous carbon support. These SA-Fe-HPC catalysts are comparable to the commercial Pt/C electrode even in acidic electrolytes for oxygen reduction reaction (ORR) in terms of the ORR activity (E =0.

Nitrogen and Phosphorus Codoped Mesoporous Carbon Derived from Polypyrrole as Superior Metal-Free Electrocatalyst toward the Oxygen Reduction Reaction.

To replace high-cost platinum group metal (PGM) electrocatalysts for oxygen reduction reaction (ORR) that is the crucial cathode reaction in fuel cell technology and metal-air battery, the development of low-cost and high-efficiency non-PGM catalysts for ORR has attracted much attention during the past decades. As one of the promising candidates, N-doped carbon is highly desirable for its strong designability and outstanding catalytic activity and stability. In this work, a facile and rational strategy is demonstrated for preparation of N,P-codoped mesoporous carbon (N,P-MC) for ORR by the direct pyrolysis treatment of polypyrrole with phytic acid as P-dopant and polystyrene sphere as template.