Tracking-seq reveals the heterogeneity of off-target effects in CRISPR-Cas9-mediated genome editing.

The continued development of novel genome editors calls for a universal method to analyze their off-target effects. Here we describe a versatile method, called Tracking-seq, for in situ identification of off-target effects that is broadly applicable to common genome-editing tools, including Cas9, base editors and prime editors. Through tracking replication protein A (RPA)-bound single-stranded DNA followed by strand-specific library construction, Tracking-seq requires a low cell input and is suitable for in vitro, ex vivo and in vivo genome editing, providing a sensitive and practical genome-wide approach for off-target detection in various scenarios.

Shared Latent Membership Enables Joint Shape Abstraction and Segmentation With Deformable Superquadrics.

Part-level 3D shape representations are crucial to shape reasoning and understanding. Two key sub-tasks are: 1) shape abstraction, creating primitive-based object parts; and 2) shape segmentation, finding partition-based object parts. However, for 3D object point clouds, most advanced methods produce parts relying on task-specific priors, such as similarity metrics and primitive geometries, resulting in misleading parts that deviate from semantics.

Dual-role transcription factors stabilize intermediate expression levels.

Precise control of gene expression levels is essential for normal cell functions, yet how they are defined and tightly maintained, particularly at intermediate levels, remains elusive. Here, using a series of newly developed sequencing, imaging, and functional assays, we uncover a class of transcription factors with dual roles as activators and repressors, referred to as condensate-forming level-regulating dual-action transcription factors (TFs). They reduce high expression but increase low expression to achieve stable intermediate levels.

A Modular Neural Motion Retargeting System Decoupling Skeleton and Shape Perception.

Motion mapping between characters with different structures but corresponding to homeomorphic graphs, meanwhile preserving motion semantics and perceiving shape geometries, poses significant challenges in skinned motion retargeting. We propose M-R ET, a modular neural motion retargeting system to comprehensively address these challenges. The key insight driving M-R ET is its capacity to learn residual motion modifications within a canonical skeleton space.

Beyond Pattern Variance: Unsupervised 3-D Action Representation Learning With Point Cloud Sequence.

This work pays the first research effort to address unsupervised 3-D action representation learning with point cloud sequence, which is different from existing unsupervised methods that rely on 3-D skeleton information. Our proposition is built on the state-of-the-art 3-D action descriptor 3-D dynamic voxel (3DV) with contrastive learning (CL). The 3DV can compress the point cloud sequence into a compact point cloud of 3-D motion information.

DTCM: Joint Optimization of Dark Enhancement and Action Recognition in Videos.

Recognizing human actions in dark videos is a useful yet challenging visual task in reality. Existing augmentation-based methods separate action recognition and dark enhancement in a two-stage pipeline, which leads to inconsistently learning of temporal representation for action recognition. To address this issue, we propose a novel end-to-end framework termed Dark Temporal Consistency Model (DTCM), which is able to jointly optimize dark enhancement and action recognition, and force the temporal consistency to guide downstream dark feature learning.

NeRFPlayer: A Streamable Dynamic Scene Representation with Decomposed Neural Radiance Fields.

Visually exploring in a real-world 4D spatiotemporal space freely in VR has been a long-term quest. The task is especially appealing when only a few or even single RGB cameras are used for capturing the dynamic scene. To this end, we present an efficient framework capable of fast reconstruction, compact modeling, and streamable rendering.

Consistent 3D Hand Reconstruction in Video via Self-Supervised Learning.

We present a method for reconstructing accurate and consistent 3D hands from a monocular video. We observe that the detected 2D hand keypoints and the image texture provide important cues about the geometry and texture of the 3D hand, which can reduce or even eliminate the requirement on 3D hand annotation. Accordingly, in this work, we propose SHAND, a self-supervised 3D hand reconstruction model, that can jointly estimate pose, shape, texture, and the camera viewpoint from a single RGB input through the supervision of easily accessible 2D detected keypoints.

Federated Learning With Privacy-Preserving Ensemble Attention Distillation.

Federated Learning (FL) is a machine learning paradigm where many local nodes collaboratively train a central model while keeping the training data decentralized. This is particularly relevant for clinical applications since patient data are usually not allowed to be transferred out of medical facilities, leading to the need for FL. Existing FL methods typically share model parameters or employ co-distillation to address the issue of unbalanced data distribution.

Adaptive Two-Stream Consensus Network for Weakly-Supervised Temporal Action Localization.

Weakly-supervised temporal action localization (W-TAL) aims to classify and localize all action instances in untrimmed videos under only video-level supervision. Without frame-level annotations, it is challenging for W-TAL methods to clearly distinguish actions and background, which severely degrades the action boundary localization and action proposal scoring. In this paper, we present an adaptive two-stream consensus network (A-TSCN) to address this problem.

Motion-Driven Visual Tempo Learning for Video-Based Action Recognition.

Action visual tempo characterizes the dynamics and the temporal scale of an action, which is helpful to distinguish human actions that share high similarities in visual dynamics and appearance. Previous methods capture the visual tempo either by sampling raw videos with multiple rates, which require a costly multi-layer network to handle each rate, or by hierarchically sampling backbone features, which rely heavily on high-level features that miss fine-grained temporal dynamics. In this work, we propose a Temporal Correlation Module (TCM), which can be easily embedded into the current action recognition backbones in a plug-in-and-play manner, to extract action visual tempo from low-level backbone features at single-layer remarkably.

Oestrogen promotes lipids transportation through oestrogen receptor α in hepatic steatosis of geese in vitro.

Evidence has shown that oestrogen suppresses lipids deposition in the liver of mammals. However, the molecular mechanism of oestrogen action in hepatic steatosis of geese liver has yet to be determined. This study aimed to investigate the effect of oestrogen on lipid homeostasis at different states of geese hepatocytes in vitro.

Joint Hand-Object 3D Reconstruction From a Single Image With Cross-Branch Feature Fusion.

Accurate 3D reconstruction of the hand and object shape from a hand-object image is important for understanding human-object interaction as well as human daily activities. Different from bare hand pose estimation, hand-object interaction poses a strong constraint on both the hand and its manipulated object, which suggests that hand configuration may be crucial contextual information for the object, and vice versa. However, current approaches address this task by training a two-branch network to reconstruct the hand and object separately with little communication between the two branches.

Image Co-Skeletonization via Co-Segmentation.

Recent advances in the joint processing of a set of images have shown its advantages over individual processing. Unlike the existing works geared towards co-segmentation or co-localization, in this article, we explore a new joint processing topic: image co-skeletonization, which is defined as joint skeleton extraction of the foreground objects in an image collection. It is well known that object skeletonization in a single natural image is challenging, because there is hardly any prior knowledge available about the object present in the image.

3D Object Representation Learning: A Set-to-Set Matching Perspective.

In this paper, we tackle the 3D object representation learning from the perspective of set-to-set matching. Given two 3D objects, calculating their similarity is formulated as the problem of set-to-set similarity measurement between two set of local patches. As local convolutional features from convolutional feature maps are natural representations of local patches, the set-to-set matching between sets of local patches is further converted into a local features pooling problem.

MAT: Multianchor Visual Tracking With Selective Search Region.

The core prerequisite of most modern trackers is a motion assumption, defined as predicting the current location in a limited search region centering at the previous prediction. For clarity, the central subregion of a search region is denoted as the tracking anchor (e.g.

Unsupervised Learning of Optical Flow With CNN-based Non-Local Filtering.

Estimating optical flow from successive video frames is one of the fundamental problems in computer vision and image processing. In the era of deep learning, many methods have been proposed to use convolutional neural networks (CNNs) for optical flow estimation in an unsupervised manner. However, the performance of unsupervised optical flow approaches is still unsatisfactory and often lagging far behind their supervised counterparts, primarily due to over-smoothing across motion boundaries and occlusion.

3D Hand Pose Estimation Using Synthetic Data and Weakly Labeled RGB Images.

Compared with depth-based 3D hand pose estimation, it is more challenging to infer 3D hand pose from monocular RGB images, due to the substantial depth ambiguity and the difficulty of obtaining fully-annotated training data. Different from the existing learning-based monocular RGB-input approaches that require accurate 3D annotations for training, we propose to leverage the depth images that can be easily obtained from commodity RGB-D cameras during training, while during testing we take only RGB inputs for 3D joint predictions. In this way, we alleviate the burden of the costly 3D annotations in real-world dataset.

SibNet: Sibling Convolutional Encoder for Video Captioning.

Visual captioning, the task of describing an image or a video using one or few sentences, is a challenging task owing to the complexity of understanding the copious visual information and describing it using natural language. Motivated by the success of applying neural networks for machine translation, previous work applies sequence to sequence learning to translate videos into sentences. In this work, different from previous work that encodes visual information using a single flow, we introduce a novel Sibling Convolutional Encoder (SibNet) for visual captioning, which employs a dual-branch architecture to collaboratively encode videos.

Asymmetric Mapping Quantization for Nearest Neighbor Search.

Nearest neighbor search is a fundamental problem in computer vision and machine learning. The straightforward solution, linear scan, is both computationally and memory intensive in large scale high-dimensional cases, hence is not preferable in practice. Therefore, there have been a lot of interests in algorithms that perform approximate nearest neighbor (ANN) search.

Action-Stage Emphasized Spatio-Temporal VLAD for Video Action Recognition.

Despite outstanding performance in image recognition, convolutional neural networks (CNNs) do not yet achieve the same impressive results on action recognition in videos. This is partially due to the inability of CNN for modeling long-range temporal structures especially those involving individual action stages that are critical to human action recognition. In this paper, we propose a novel action-stage (ActionS) emphasized spatiotemporal Vector of Locally Aggregated Descriptors (ActionS-STVLAD) method to aggregate informative deep features across the entire video according to adaptive video feature segmentation and adaptive segment feature sampling (AVFS-ASFS).

Bmp4 inhibits goose granulosa cell apoptosis via PI3K/AKT/Caspase-9 signaling pathway.

Bone morphogenetic protein 4 (BMP4) has an important role in regulating cellular proliferation, differentiation and apoptosis. It, however, is still unclear as to the mechanisms by which BMP4 regulates the apoptosis of granulosa cells (GCs) in geese. In the present study, there was cloning of the full-length coding sequence of goose BMP4 gene, which consisted of 1212 nucleotides encoding 403 amino acids.

Minimizing Reconstruction Bias Hashing via Joint Projection Learning and Quantization.

Hashing, a widely-studied solution to the approximate nearest neighbor (ANN) search, aims to map data points in the high-dimensional Euclidean space to the low-dimensional Hamming space while preserving the similarity between original points. As directly learning binary codes can be NP-hard due to discrete constraints, a two-stage scheme, namely "projection and quantization", has already become a standard paradigm for learning similarity-preserving hash codes. However, most existing hashing methods typically separate these two stages and thus fail to investigate complementary effects of both stages.