Front Cardiovasc Med
March 2024
Stem cell-based myocardial regeneration is a frontier topic in the treatment of myocardial infarction. Manipulating the metabolic microenvironment of stem cells can influence their differentiation into cardiomyocytes, which have promising clinical applications. pH is an important indicator of the metabolic environment during cardiomyocyte development.
View Article and Find Full Text PDFThe T-box family transcription factor gene TBX20 plays a crucial role in cardiac development and function. TBX20 mutations are associated with congenital heart disease, dilated cardiomyopathy, arrhythmias, and heart failure. To further study the role of TBX20 in human heart, here we generated a homozygous TBX20 knockout (TBX20-KO) human embryonic stem cell line using the CRISPR/Cas9 system.
View Article and Find Full Text PDFIEEE J Biomed Health Inform
June 2024
Federated learning (FL) enables collaborative training of machine learning models across distributed medical data sources without compromising privacy. However, applying FL to medical image analysis presents challenges like high communication overhead and data heterogeneity. This paper proposes novel FL techniques using explainable artificial intelligence (XAI) for efficient, accurate, and trustworthy analysis.
View Article and Find Full Text PDFThe incidence of cardiovascular disease has been continuously increasing. Because cardiomyocytes (CM) are non-renewable cells, it is difficult to find appropriate CM sources to repair injured hearts. Research of human induced pluripotent stem cell (hiPSC) differentiation and maturation into CM has been invaluable for the treatment of heart diseases.
View Article and Find Full Text PDFBackground: We evaluated whether the number of myocardial infarction (MI) segments connected to the papillary muscle (PM), as assessed using cardiac magnetic resonance (CMR) with late gadolinium enhancement (LGE), predicts whether moderate ischemic mitral regurgitation (IMR) improves after isolated coronary artery bypass grafting (CABG) to guide the choice of surgical strategy.
Methods: A total of 54 patients diagnosed with coronary heart disease (CHD) complicated with moderate IMR who underwent isolated CABG were selected continuously in this retrospective study at Beijing Anzhen Hospital. All patients underwent preoperative LGE.
Background: Drawing on accumulated patient data from a hospital database, the goal of this retrospective study was to analyze cardiac function associated with global preoperative myocardial scarring assessed by cardiac magnetic resonance with late gadolinium enhancement (CMR-LGE) in patients with ischemic cardiomyopathy (ICM) after coronary artery bypass grafting (CABG).
Methods: A total of 57 patients diagnosed with ICM who underwent isolated CABG at Beijing Anzhen Hospital between September 2017 and September 2019 were enrolled in this retrospective study. All these patients underwent a preoperative CMR-LGE examination.
Background: Ventricular septal rupture (VSR) is a rare and fatal complication of myocardial infarction. Surgery is the main treatment for the condition. It is currently believed that surgery is less effective for posterior VSR than for anterior VSR.
View Article and Find Full Text PDFIschemic heart disease resulting from a myocardial infarction (MI), is a major health issue. Stem cell therapies may play an important role in this field. However, cardiomyocytes induced from stem cells are characterized by low rates of differentiation and immaturity.
View Article and Find Full Text PDFBackground: In stem cell therapy, due to the lack of an effective carrier, a large number of transplanted stem cells are lost and die. Therefore, finding a suitable carrier has become a further direction of stem cell therapy.
Objective: In research on the co-culture of polycaprolactone (PCL) with 1,1'-Dioctadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiI) labeled bone marrow mesenchymal stem cells (BMSCs), we observe the effect of materials on the growth and proliferation of DiI labeled stem cells, and the effect of DiI labeling on patch preparation, so as to find a kind of biomaterial suitable for the growth and proliferation of BMSCs, and find a suitable cell carrier for stem cell therapy of myocardial infarction and in vivo tracing.
Stem cells have significant potential use in tissue regeneration, especially for treating cardiac diseases because of their multi-directional differentiation capability. By mimicking the in vivo physiological environment of native cardiomyocytes during their development and maturation, researchers have been able to induce pluripotent stem cell-derived cardiomyocytes (PSC-CMs) at high purity. However, the phenotype of these PSC-CMs is immature compared with that of adult cardiomyocytes.
View Article and Find Full Text PDFSpectrum sensing (SS) exhibits its advantages in the era of Internet of Things (IoT) due to limited spectrum resource and a lower utilization rate of authorized spectrum. In consequence, the performance improvement of SS seems a matter of great significance for the development of wireless communication and IoT. Motivated by this, this paper is devoted to multi-slot based SS in specialty and several important conclusions are drawn.
View Article and Find Full Text PDFCongenitally corrected transposition of the great arteries (CTGA) is a rare congenital heart disease. In patients with functional CTGA with circumflex artery occlusion and mitral regurgitation (MR), the right ventricle functions as the left ventricle. Coronary artery bypass grafting with mitral valve replacement is an effective treatment for CTGA with concomitant myocardial infarction (MI) and MR.
View Article and Find Full Text PDFZhonghua Xin Xue Guan Bing Za Zhi
December 2015
Objective: To observe the proliferation and differentiation capacities of mouse induced pluripotent stem cells (miPSCs) cultured in 3-hydroxybutyrate-co-3-hydroxyhexanoate(PHBHHx) three-dimensional films three-dimension films for the purpose of finding a suitable polymeric biomaterials for forming myocardial patches.
Methods: miPSCs were recovered, passaged, cultured and identified, then miPSCs divided into the experimental and control groups. MiPSCs in the experimental group were cultured with PHBHHx three-dimension films.
Identifying a suitable polymeric biomaterial for myocardial patch repair following myocardial infarction, cerebral infarction, and cartilage injury is essential. This study aimed to investigate the effect of the novel polymer material, poly3-hydroxybutyrate-co-3-hydroxyhexanoate, on the adhesion, proliferation, and differentiation of mouse-induced pluripotent stem cells in vitro. Mouse-induced pluripotent stem cells were isolated, expanded, and cultured on either two-dimensional or three-dimensional poly3-hydroxybutyrate-co-3-hydroxyhexanoate films (membranes were perforated to imitate three-dimensional space).
View Article and Find Full Text PDFBackground: The aim of this study was to evaluate the medium- and long-term efficacy of loading statins after coronary artery bypass grafting (CABG) by comparing using a loading dose of statins or a regular dose of statins in patients undergoing CABG.
Methods: We conducted a literature search of numerous databases for randomized controlled trials on the efficacy of a loading dose of statins in patients undergoing CABG. The quality of included studies was evaluated according to the Newcastle-Ottawa Scale.
This comparative study investigates the method, efficiency, and anti-hypoxic ability of cardiomyocytes, directionally induced from human (h) and mouse (m) embryonic stem cells (ESCs). hESCs were induced into cardiomyocytes by suspension culture, without inducers, or adherent culture using the inducers activin A and BMP4. mESCs were induced into cardiomyocytes by hanging-drop method, without inducers or induced with vitamin C.
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