The practicability of different preparation of mandibular molar restored by modified endocrown with intracanal extension: Computational analysis using finite element models.

Background And Objective: Post-core-crown (PCC) and endocrown are two common restorative methods for severely damaged molars, but exhibit disadvantages. This study aimed to explore the practicability of modified endocrown with a 2 mm intracanal extension (MED) to restore defective teeth using finite element analysis (FEA).

Methods: Five groups of numerical models of mandibular molars restored by three MEDs, a PCC, and a routine endocrown after root canal treatment were devised by FEA software.

Single-cell RNA analysis of chemokine expression in heterogeneous CD14 monocytes with lipopolysaccharide-induced bone resorption.

Lipopolysaccharide (LPS)-induced bone resorption has normally been found in inflammatory bone diseases, but the underlying mechanism is currently unclear. Since LPS binds to CD14 and activates Toll-like receptor 4 (TLR4) in monocytes, the present study focused on CD14 monocytes and observed their responses after LPS treatment during the progression of local bone destruction. CD14 monocytes were obtained from human peripheral blood mononuclear cells (PBMCs) by magnetic cell separation (MACS), and their classification was confirmed by fluorescence-activated cell sorting (FACS).