Publications by authors named "Junlong Meng"

Article Synopsis
  • The copper transport (COPT/Ctr) family helps organisms manage copper levels and is mainly studied in plants, while its role in edible mushrooms is less explored.
  • This study focused on the CCMSSC00389 strain, demonstrating that adding copper ions enhances mycelial temperature tolerance, cell membrane integrity, and density.
  • Four genes were identified, showing different expression patterns under temperature stress, and the OE- strain improved heat resistance and recovery of mycelia, suggesting it regulates copper homeostasis for better cell membrane stability.
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The (Button mushroom) stands out as one of the most prolific edible fungi which offers robust flavor and nutrition. Nonetheless, this mushroom contains high moisture levels and intense respiration. Without appropriate postharvest preservation techniques, the button mushroom readily experiences browning and senescence.

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Polysaccharides could be used to form the network structure of casein (CA) gel, and affect its gelling properties. Auricularia auricula polysaccharide (AAP) has good gel properties and activity, however, how the AAP affects gelling properties of CA gels remains unclear. In this study, AAP and CA were acid-induced by glucono-δ-lactone (GDL) to prepare composite gels for curcumin loading.

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Morels ( spp.) are valuable and rare edible mushrooms with unique flavors and high nutritional value. White mold disease occurring during cultivation has seriously affected the quality and yield of morels in China.

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A novel species of earthstar from China, is described. Phylogenetic analyses based on sequences of the nuclear ribosomal DNA internal transcribed spacer (ITS), large subunit nuclear ribosomal RNA (nrLSU), and subunit 6 of ATP synthase () regions showed that the species belongs to subsect. in sect.

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  • - Lead is a harmful environmental pollutant that can accumulate in the kidneys and cause damage, and the effects of Sparassis latifolia polysaccharide (SLP) on lipid metabolism abnormalities due to lead exposure are not well understood.
  • - This study used mice to investigate kidney damage and changes caused by lead, examining key signaling pathways through lipidomics and transcriptomics to identify affected metabolites and genes linked to oxidative stress, inflammation, and autophagy.
  • - Results showed that SLP treatment regulated abnormal lipid metabolism in the kidneys primarily by reducing oxidative stress and its related impacts on autophagy and inflammation, suggesting potential for SLP as a therapeutic intervention for lead-induced kidney injury.
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polysaccharide (MIP) has been proven to have obvious hypoglycemic effects on mice with type 2 diabetes (T2DM). This study looked at the functional and rheological characteristics of MIP, and investigated the effects of MIP on the human fecal microbiota through in vitro fermentation experiments. The outcomes demonstrate the excellent oil-holding capacity, emulsifying, foaming, and rheological characteristics of MIP.

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This study aimed to develop novel nanoparticles that can serve as an excellent oil-in-water (O/W) Pickering stabilizer. The polysaccharide-protein complex nanoparticles (PPCNs-20 and PPCNs-40) were prepared at different ultrasonication amplitudes (20 % and 40 %, respectively) from the polysaccharide-protein complexes (PPCs) which were extracted from the residue of Clitocybe squamulose. Compared with PPCs and PPCNs-20, the PPCNs-40 exhibited dispersed blade and rod shape, smaller average size, and larger zeta potential, which indicated significant potential in O/W Pickering emulsion stabilizers.

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  • The study compares the effects of hot air drying (HD), infrared radiation drying (ID), and vacuum freeze drying (VD) on the quality and properties of dried products made from fresh materials.
  • VD exhibited the highest rehydration rate and maintained better appearance and original color, despite having a weaker aroma compared to HD.
  • Analysis showed that vacuum freeze-dried samples had superior structure and antioxidant properties, making it the preferred drying method for preserving quality.
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In this study, we extracted the polysaccharides from C. militaris fruiting bodies (CFIPs), mycelial intracellular polysaccharides (CMIPs), and fermentation broth extracellular polysaccharides (CFEPs) to investigate their physicochemical properties, antioxidant capacities, and effects on oxazolone-induced zebrafish ulcerative colitis (UC). Our results revealed differences in monosaccharide composition and surface structure among CFIPs, CMIPs, and CFEPs.

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This study aimed to assess the effects of polysaccharides extracted from Hericium erinaceus fruiting bodies (HEFPs) on the inflammatory response to oxidative stress in a mouse model of ulcerative colitis (UC) induced by ingestion of dextran sodium sulfate. The results indicated reduced oxidative damage in the HEFPs groups, as evidenced by significantly decreased malondialdehyde levels and significantly increased levels of the antioxidant enzymes superoxide dismutase and catalase in colon homogenates, compared with those in the Model Control (MC) group. Additionally, compared with the levels in the MC group, the levels of the pro-inflammatory factors IL-6, IL-1β, and TNF-α in the positive-control (PC) and HEFPs groups were significantly lower, and that of the anti-inflammatory factor IL-10 was significantly higher.

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  • Researchers extracted four types of N. aurantialba polysaccharides (NAPs) using various methods: enzyme-assisted, ultrasound-assisted, alkali, and acid, with different yields ranging from 12.77% to 26.05%.
  • Each NAP had unique properties, including differences in monosaccharide composition and structural characteristics, with U-NAP and Al-NAP exhibiting a triple helix structure.
  • The study reveals how sequential extraction enhances polysaccharide purity and solubility, providing valuable insights for their high-value applications.
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Taking rural dispersed sewage for research objects, the treatment effect and microbial community structure characteristics of a bio filter (BF) reactor was studied. At fixed time and location, the removal efficiencies of common pollutants were investigated. By using high-throughput sequencing method, the heterogeneities of microbial community structure in fillers and plant roots were analyzed.

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To study the gel-forming properties of polysaccharide from the fruiting body of Clitocybe squamulosa (CSFP) and its degradation product (UH-CSFP), the changes in steady-state and dynamic rheological properties of CSFP and UH-CSFP under different conditions (polysaccharide mass fraction, temperature, pH, and salt ion concentration) were studied. Polysaccharides with good gel-forming properties were selected and mixed with common edible thickeners (gelatin, guar gum, and locust bean gum), after which the properties of the composite gel were assessed. The steady-state rheological results showed that CSFP and UH-CSFP were pseudoplastic fluids, their apparent viscosity decreased with increasing temperature, the viscosity was greatest when the pH was 7.

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  • - This study examines how different processing methods of Naematelia aurantialba (NA) impact the gelatinization and rheological properties of corn starch (CS) and edible cassava starch (ECS), specifically in the context of making starch soft candy.
  • - Rheological tests indicated that both CS-NA and ECS-NA showed elastic behavior, with varying elastic moduli, suggesting that the addition of NA affects the texture properties of the starch.
  • - The optimal recipe for the soft candy was identified as having specific amounts of NA, sugar, ECS and CS ratio, citric acid, red date powder, and water, leading to a stable product that maintains its quality over two days.
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Article Synopsis
  • - The research focuses on the regulatory mechanisms of growth and development in a type of edible mushroom, specifically examining the function of the MAC1 protein, which interacts with copper and influences gene expression.
  • - Two transcription factor genes were identified, and experiments with overexpression (OE) and RNA interference (RNAi) strains revealed that these genes play a role in heat stress tolerance, particularly at 32 °C and 40 °C.
  • - The findings indicate distinct functional roles for the two genes during different growth stages, with one gene promoting rapid primordia formation and shortening cultivation time, highlighting their importance in mushroom development.
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This study comparatively evaluated the effects of the commonly used six extraction methods (acidic, alkaline, enzymatic, ultrasonic, high-pressure, and microwave) on the physico-chemical properties, processing characteristics, and biological activities of polysaccharides from Clitocybe squamulosa (CSFPs). The results show that polysaccharides extracted using an enzyme-assisted extraction method has a relatively high extraction yield (4.46 ± 1.

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The browning and associated toughening of fruiting body stipes are the main causes of declines in the commercial production of yellow . The dynamic metabolic changes from the top to bottom stipe sections of yellow fruiting bodies were investigated by integrating physiological, transcriptomic, and metabolomic analyses. The results indicated that oxidative stress levels gradually increased accompanying the degree of tissue browning and toughening from the top to bottom sections of stipes.

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In order to obtain the optimal conditions for ammonia nitrogen (AN) wastewater treatment by bio filter (BF), the effects of ratio of carbon to nitrogen (C/N), pH, and hydraulic load (HL) on the AN degradation were studied by Response Surface Methodology (RSM). Central Composite Design (CCD) experiments were conducted, and the response of the AN removal rates were fitted to a second-order polynomial model. The analysis of variance showed that the model was accurate and reliable.

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Article Synopsis
  • MYB transcription factors (TFs) play an important role in plant stress responses and growth, but their specific impacts on heat stress and development are not fully understood.
  • Researchers used RNA interference (RNAi) and overexpression (OE) techniques to create mutant strains and found that these mutants showed improved performance under various heat stress conditions (32 °C, 36 °C, and 40 °C).
  • The study concluded that certain MYB TFs enhance mycelium's heat stress response and promote the growth and recovery of plants, while one specific TF inhibits these processes.
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is widely cultivated in China. HO, as a signaling molecule, can regulate the formation of cap color, but its regulatory pathway is still unclear, severely inhibiting the breeding of dark-colored strains. In this study, 614 DEGs specifically regulated by HO were identified by RNA-seq analysis.

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Changes in the functional properties and structures of protein isolate (CSPI) in the process of freeze-thaw (F-T) cycles were explored. Remarkable alterations and the reduced content of protein ordered structure were revealed through structural analysis of CSPI after F-T treatments. The surface hydrophobicity and free sulfhydryl content of CSPI first increased and then decreased.

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The aim of this study was to establish a human digestion model to explore the degradation characteristics of a novel high-purity polysaccharide from (CSFP2). The results showed that the content of reducing sugars ( ) of CSFP2 increased from 0.13 to 0.

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Sparassis latifolia polysaccharides (SLPs) can regulate inflammatory cytokines. However, little is known about the regulation mechanism of SLPs on colon cancer. In this study, we investigated the mechanism of SLPs on metabolism in mice with colon cancer.

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is a famous edible and medicinal fungus in China and Asia. The molecular basis of morphogenesis and synnemal formation needs to be understood in more detail because this is the main source of biomass production in . In the present study, a fruiting body formation-related gene with a glycosylphosphatidylinositol (GPI) anchoring protein (GPI-Ap) gene homolog was identified by screening random insertion mutants.

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