In vivo imaging to trace the dissemination of Aeromonas hydrophila in Common carp (Cyprinus carpio) after intestinal infection.

Aeromonas hydrophila (A. hydrophila) is a pathogenic bacterium that often causes serious economic losses to aquaculture and fisheries. Its infection and pathogenesis of fish are currently not fully understood.

Acid-Controlled Fabrication of Multicolor Carbon Dots with Switchable Organelle-Targeting Capability for Visualizing Organelle Interactions.

Synchronous regulation of the photoluminescence and physicochemical characteristics of multicolor carbon dots (CDs) can fully realize their application potential in multicomponent imaging. Herein, by utilizing an acid-regulated synthetic strategy, green-emissive and orange-emissive CDs that target lipid droplets (LDs) and mitochondria (Mito) have been developed for fluorescence visualization of LD-Mito interactions. The finding of different molecular fluorophores reveals that the precursor undergoes different reaction pathways in neutral and acidic conditions, which alters the size of sp-conjugated domain and surface properties for the successful regulation of photoluminescence properties and organelle-targeting ability.

Evaluation of a novel ensemble model for preoperative ovarian cancer diagnosis: Clinical factors, O-RADS, and deep learning radiomics.

Background: Accurate early diagnosis of ovarian cancer is crucial. The objective of this research is to create a comprehensive model that merges clinical variables, O-RADS, and deep learning radiomics to support preoperative diagnosis and assess its efficacy for sonographers.

Materials And Methods: Data from two centers were used: Center 1 for training and internal validation, and Center 2 for external validation.

Integrating radiomics into predictive models for low nuclear grade DCIS using machine learning.

Predicting low nuclear grade DCIS before surgery can improve treatment choices and patient care, thereby reducing unnecessary treatment. Due to the high heterogeneity of DCIS and the limitations of biopsies in fully characterizing tumors, current diagnostic methods relying on invasive biopsies face challenges. Here, we developed an ensemble machine learning model to assist in the preoperative diagnosis of low nuclear grade DCIS.

UPLC-MS/MS method for simultaneous determination of pyrantel, praziquantel, febantel, fenbendazole and oxfendazole in dog plasma and its application to a bioequivalence study.

A rapid, sensitive and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the simultaneous quantitation of pyrantel (PYR), praziquantel (PRA), febantel (FBT) and its active metabolites, fenbendazole (FEN) and oxfendazole (OXF) in dog plasma. Fenbendazole-D3 (FEN-D3) was used as an internal standard (IS). The analytes and IS were prepared using simple protein precipitation (PP) combined with liquid-liquid extraction (LLE).

24-Hour Movement Behavior and Health-Related Physical Fitness in College Students: A Compositional Data Analysis.

Background: Sedentary behavior and physical inactivity are primary factors contributing to college students' continued decline in health-related physical fitness (HRPF). This study aims to examine the associations between 24-h movement behavior and HRPF, and explore the dose-response relationship between the reallocation of movement behavior time and HRPF.

Method: In total, 1032 college students (649 males and 383 females) aged 19.

Investigation on Rational Utilization of Medicinal Plant Chang Leaf and Bark at Different Developmental Stages.

Chang is an extremely valuable and endangered medicinal plant. To investigate the exploitation and rational utilization of , this study conducted metabolomics analysis of the leaves and bark of artificially cultivated at different developmental stages. These metabolites were detected and identified by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) technology, and then univariate statistical analyses, multivariate pattern analyses, and pathway analyses were carried out.

Synthesis and in vitro and in vivo evaluation of novel bivalent quinolines as antitumor agents via targeting autophagy in cervical cancer.

A series of novel bivalent quinolines with a spacer of four to six methylene units between the phenoxy group in the position-7 and various substituents in the position-4 of quinoline skeleton, respectively, were synthesized and evaluated as anticancer agents. The data showed that the majority of the compounds had significant antiproliferative activity with IC values less than 50 μM against human cancer cell lines. Among them, compound 4b exhibited the strongest antiproliferative activity against HCT116, A549, BGC823, HeLa and MCF-7 cell lines with an IC values of 0.

Electroacupuncture regulates FTO/Nrf2/NLRP3 axis-mediated pyroptosis in cerebral ischemia-reperfusion injury.

Electroacupuncture (EA) demonstrates neuroprotective in cerebral ischemia-reperfusion (I/R) injury. N6-methyladenosine (m6A) is found to contribute to the pathogenesis of neurological conditions recently. The objective of this study is to investigate the effects of EA on m6A and related mechanism in cerebral I/R injury.

Balanced plant helper NLR activation by a modified host protein complex.

Nucleotide-binding leucine-rich repeat (NLR) receptors play crucial roles in plant immunity by sensing pathogen effectors. In Arabidopsis, certain sensor NLRs function as NADases to catalyse the production of second messengers, which can be recognized by enhanced disease susceptibility 1 (EDS1) with its partner senescence-associated gene 101 (SAG101), to activate helper NLR N requirement gene 1 (NRG1). A cryoelectron microscopy structure shows that second-messenger-activated EDS1-SAG101 mainly contacts the leucine-rich repeat domain of NRG1A to mediate the formation of an induced EDS1-SAG101-NRG1A complex.

Burden of female infertility in 204 countries and territories, 1990-2021: results from the Global Burden of Disease Study 2021.

Objective: To explore the global burden of female infertility from 1990 to 2021 by examining trends in prevalence and years lived with disability (YLD).

Methods: Data from the Global Burden of Disease Study 2021 (GBD 2021) were analyzed with a focus on the prevalence and YLD of female infertility in women aged 15-49 years. Statistical models were used to estimate ASPRs and YLD across regions and countries.

Molecular characterization of AMP-activated protein kinase (AMPK) α1/α2 from Cyprinus carpio and its roles in glucolipid metabolism and immune response.

AMPKα1 and AMPKα2, key kinases in regulating energy homeostasis, have not been previously cloned or characterized in common carp (Cyprinus carpio). This study identified the open reading frame (ORF) sequences of ampkα1 (1722 bp, encoding 573 amino acids) and ampkα2 (1659 bp, encoding 552 amino acids) through homologous cloning. Sequence alignment and phylogenetic analysis showed a high similarity of both genes with fish homologs.

Ultrasound radiomics-based logistic regression model for fibrotic NASH.

Background: Those who have severe fibrosis (F2 ≥ 2 stage) are at the greatest risk for the advancement of the illness among non-alcoholic fatty liver patients. To forecast the non-alcoholic steatohepatitis (NASH) probability accompanied by significant fibrosis, we propose to develop and validate a nomogram liver imaging reporting and data system, providing robust evidence for preventing and treating clinical liver diseases.

Methods: The study used SD rats to create a model of hepatic steatosis and fibrosis by feeding them a high-fat diet and injecting Ccl4 subcutaneously.

NSUN2 Knockdown Promotes the Ferroptosis of Colorectal Cancer Cells Via m5C Modification of SLC7A11 mRNA.

The high occurrence and death rates of colorectal cancer (CRC) make it a major health concern. Recent studies have identified NOP2/Sun RNA methyltransferase family member 2 (NSUN2), an RNA methyltransferase, as a key regulator in various tumor types. However, how exactly NSUN2-mediated m5C alteration affects CRC is still a mystery.

Research hotspots and frontiers of endoplasmic reticulum in glomerular podocytes: a bibliometric and visual analysis from 2005 to 2023.

Background: The glomerular podocyte endoplasmic reticulum is a critical component in renal function, yet its research landscape is not fully understood. This study aims to map the existing research on podocyte endoplasmic reticulum by analyzing publications in the Web of Science Core Collection (WOSCC) from the past 19 years.

Methods: We conducted a bibliometric analysis using Citespace, VOSviewer, the Metrology Literature Online platform, and the Bibliometrix software package to visualize and interpret the data from WOSCC.

Comprehensive analysis of protein post-translational modifications reveals PTPN2-STAT1-AOX axis-mediated tumor progression in hepatocellular carcinomas.

Hepatocellular carcinoma (HCC) is a common malignant tumor. Although the proteomics of HCC is well studied, the landscape of post-translational modifications (PTMs) in HCC is poorly understood. The PTMs themselves and their crosstalk might be deeply involved in HCC development and progression.

Effects of Sensory-Based Interventions on Delirium Prevention in Critically Ill Patients: A Systematic Review and Meta-Analysis.

Objective: To identify, appraise and synthesize current evidence on different sensory-based interventions on delirium prevention in critically ill patients.

Data Sources: A comprehensive electronic literature search was performed in the PubMed, Embase, Cumulative Index of Nursing and Allied Health Literature, Cochrane Library, China Biology Medicine, China National Knowledge Infrastructure, Wanfang and WeiPu databases from inception to 2 June 2022. The data were updated on 24 December 2022.

Accumulation, physiological and proteomic analyses of Suaeda salsa under cadmium exposure.

Suaeda salsa, the dominant herbaceous plant in the high salinity areas of Asia, can even grow in the heavy metal polluted region. In order to illustrate the mechanisms of Cd (cadmium) tolerance in S. salsa, the accumulation, physiological and proteomic characters under two different concentrations of Cd exposure were investigated in this study.

Disentangled Active Learning on Graphs.

Active learning on graphs (ALG) has emerged as a compelling research field due to its capacity to address the challenge of label scarcity. Existing ALG methods incorporate diversity into their query strategies to maximize the gains from node sampling, improving robustness and reducing redundancy in graph learning. However, they often overlook the complex entanglement of latent factors inherent in graph-structured data.

The impact of aerobic exercise on depression in young people: A meta regression and meta-analysis.

Objective: This systematic review aimed to 1) update the existing evidence on the antidepressant effects of aerobic exercise (AE) in youth. 2) Investigate any potential dose‒response relationships between AE interventions and depressive symptom reduction. 3) Provide evidence-based insights to inform future research and clinical depression treatment.

Tunable Beam Steering Metasurface Based on a PMN-PT Crystal with a High Electro-Optic Coefficient.

Existing tunable optical metasurfaces based on the electro-optic effect are either complex in structure or have a limited phase modulation range. In this paper, a simple rectangular metasurface structure based on a Pb(MgNb)O-PbTiO (PMN-PT) crystal with high electro-optic coefficient of 120 pm/V was designed to demonstrate its electrically tunable performance in the optical communication band through simulations. By optimizing the structure parameters, a tunable metasurface was generated that can induce a complete 2π phase shift for beam deflection while maintaining relatively uniform transmittance.

Experimental demonstration of 8190-km long-haul semiconductor-laser chaos synchronization induced by digital optical communication signal.

Common-signal-induced synchronization of semiconductor lasers have promising applications in physical-layer secure transmission with high speed and compatibility with the current fiber communication. Here, we propose an ultra-long-distance laser synchronization scheme by utilizing random digital optical communication signal as the common drive signal. By utilizing the long-haul optical coherent communication techniques, high-fidelity fiber transmission of the digital drive can be achieved and thus ultra-long-distance synchronization is expected.

synthesis of an Al and V co-doped TiO NTA interlayer-enhanced PbO composite for efficient zinc electrowinning.

The high overpotential of the oxygen evolution reaction (OER) and the strong corrosion of the anode are the main problems currently faced by the zinc hydrometallurgical process. This study achieved the successful synthesis of titanium dioxide nanotubes doped by Al and V on a TC4 alloy. Subsequently, a composite electrode, TC4/AVTN-7/PbO-ZrO-CoO, was prepared utilizing composite electrodeposition.

Synthesis of Hollow Zn/ZSM-5 Nanosheets via Different Alkali Treatments with ZIF-8 as a Zn Source for Efficient Aromatization of Methanol.

Diffusion limitations and monofunctional acidity of ZSM-5 molecular sieves affect the catalyst stability and aromatic yield in the reaction of methanol to aromatics (MTA). In this study, based on ZSM-5 nanosheets as parent molecular sieves, Zn-modified hollow ZSM-5 nanosheets were obtained after hydrothermal treatment by adding ZIF-8 or zinc nitrate as a source of Zn while treating with different types and concentrations of alkali solutions. The physical and chemical properties of the fabricated samples and their catalytic performance of methanol aromatization were systematically investigated by a combination of XRD, TEM, N adsorption-desorption, NH-TPD, Py-IR, Al MAS NMR, Si MAS NMR, XPS, and TG characterization analyses and MTA experimental evaluation.