[Screening and identification of key miRNAs in post-transcriptional regulation of CART in the bovine hypothalamus].

This study aimed to explore the roles of microRNAs (miRNAs) in the post-transcriptional regulation of cocaine- and amphetamine-regulated transcript (CART) peptide in the bovine hypothalamus and to screen key regulatory miRNAs. Targetscan was used to predict the potential miRNAs binding to 3' untranslated regions (3'UTR). Bioinformatics analysis predicted 7 miRNA binding sites in the bovine 3'UTR, which were bta-miR-377, bta-miR-331-3p, bta-miR-491, bta-miR-493, bta-miR-758, bta-miR-877, and bta-miR-381, respectively.

[Screening of the lncRNA SNHG3 regulating expression in the bovine hypothalamus].

This study aimed to screen for the long non-coding RNA (lncRNA) small nucleolar RNA host gene 3 (SNHG3) capable of regulating the expression of cocaine- and amphetamine-regulated transcriptional peptide (CART) in the bovine hypothalamus and elucidate the underlying mechanism. StarBase v2.0, NCBI, and DIANA tools were used to predict the lncRNAs targeting -381 and -491, which were responsible for inhibiting expression.

[Effect of Aging on Stabilization of Cd Through Biochar Use in Alkaline Soil of Bayan Obo Mining Area].

Although biochar has been widely used in the remediation of heavy metal pollution in acidic and neutral soils, less attention has been paid to whether biochar will alter its structural properties and the ability to retain heavy metals after different degrees of aging in alkaline soils. In this study, two artificial accelerated aging methods (freeze-thaw cycle and dry-wet cycle) and a short-term natural aging method were used to simulate the aging process of biochar prepared from corn stalk. We investigated the changes in the soil pH and bioavailability, total content, and transformation of Cd before and after aging treatments.

Improved equations to estimate GFR in Chinese children with chronic kidney disease.

Background: There is currently no specific equation for estimating glomerular filtration rate (GFR) in Chinese children with chronic kidney disease (CKD). The commonly used equations are less robust than expected; we therefore sought to derive more appropriate equations for GFR estimation.

Methods: A total of 751 Chinese children with CKD were divided into 2 groups, training group (n = 501) and validation group (n = 250).

Selection of optimal models for predicting growth stress in Artemisia desertorum by comparison of linear regression and multiple neural networks: Take the construction of a green mine in the Bayan Obo mine as an example.

In recent years, more and more countries are focusing on the control of mining sites and the surrounding ecological environment, and the new environmental concept of green mines has been proposed. By investigating the ecological background of a mine site, pollution and ecological imbalances in the mine can be predicted, managed or transformed. This study investigated the effects of rare earth elements on plant growth in the Baotou Bayan Obo Rare Earth Mine and evaluated soil contamination and subsequent remediation through the measured plant height.