Rotating cell culture system-induced injectable self-assembled microtissues with epidermal stem cells for full-thickness skin repair.

Epidermal stem cells (EpSCs) are crucial for wound healing and tissue regeneration, and traditional culture methods often lead to their inactivation. It is urgent to increase the yield of high quality EpSCs. In this study, primary EpSCs were isolated and cultured in a serum-free, feeder-free culture system.

Effects of chronic unpredictable mild stress-induced depression on bitter taste receptor expression in mice.

Objective: With the rapid increase in the pace of life, people are facing increasing pressures of all kinds, and depression has gradually become a serious psychological disorder in human society, strongly affecting normal social and physiological activities. Depression can disrupt an individual's taste perception and potentially result in taste disorders by affecting and altering taste receptors. This disruption can consequently impact their food preferences and overall eating experiences.

Comparison of Implantation Accuracy Among Different Navigated Approaches: A Systematic Review and Network Meta-analysis.

Purpose: Dental implants are a common treatment method after tooth loss, the accuracy of which directly affects efficacy and stability. Through a network meta-analysis, this study compared the accuracy of different modalities of implant placement: dynamic navigation (DN), fully guided static navigation (FG), partially guided static navigation (PG), and free handed (FH).

Materials And Methods: This study followed the Preferred Reporting Items for Meta-Analyses (PRISMA) guidelines.

treatment as adjuvant for geographic tongue and fissured tongue with gastritis: case report.

Background: Geographic tongue and fissured tongue are both common oral mucous membrane diseases relatively. The treatment of this disease is typically causative therapy because of the unknown etiology, and other afflictions. Gastrointestinal disease is one of the factors that induce geographic tongue with a fissured tongue.

The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization.

Background: Repairing critical-sized bone defects secondary to traumatic or tumorous damage is a complex conundrum in clinical practice; in this case, artificial scaffolds exhibited preferable outcomes. Bredigite (BRT, CaMgSiO) bioceramic possesses excellent physicochemical properties and biological activity as a promising candidate for bone tissue engineering.

Methods: Structurally ordered BRT (BRT-O) scaffolds were fabricated by a three-dimensional (3D) printing technique, and the random BRT (BRT-R) scaffolds and clinically available β-tricalcium phosphate (β-TCP) scaffolds were compared as control groups.

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization.

Mineralized collagen has been introduced as a promising barrier membrane material for guided bone regeneration (GBR) due to its biomimetic nanostructure. Immune interaction between materials and host significantly influences the outcome of GBR. However, current barrier membranes are insufficient for clinical application due to limited mechanical or osteoimmunomodulatory properties.

Soft X-ray-Enhanced Reactive Oxygen Species Generation in Mesoporous Titanium Peroxide and the Application in Tumor Synergistic Therapy.

Mesoporous titanium peroxide TiO nanospheres with a high surface area are synthesized for the application of an advanced drug system. The mesoporous TiO nanospheres have a high specific surface area of 681.89 m/g and suitable pore size (∼3 nm) that can effectively upload doxorubicin (DOX) and possesses a high drug storage capacity of 146.

Inhibition of the negative effect of high glucose on osteogenic differentiation of bone marrow stromal cells by silicon ions from calcium silicate bioceramics.

Human bone marrow stem cells (hBMSCs) are exploited for miscellaneous applications in bone tissue engineering where they are mainly used as seed cells. However, high glucose (HG) environment has negative impacts on the proliferation and osteogenic differentiation of hBMSCs, thus reducing the bone formation in diabetic patients. In our former research works, we discovered that silicon (Si) ions extracted from silicate-based bioceramics are able to stimulate the proliferation and osteogenic differentiation of hBMSCs under normal culture condition.

Sudden sensorineural hearing loss (SSHL) following a local anesthetic dental procedure.

Acute sensorineural hearing loss is an uncommon phenomenon in dentistry. We describe the case of a 79-year-old male who presented with acute sensorineural hearing loss occurring 2 days after a tooth extraction procedure under local anesthesia. Possible mechanisms are discussed.

CKIP-1 regulates the immunomodulatory function of mesenchymal stem cells.

Mesenchymal stem cells (MSCs) are self-renewing multipotent cells with immunoregulatory function, which makes them attractive candidates for regenerative medicine. However, the detailed mechanisms of their immunomodulatory capacity are not fully characterized. Here, we found that casein kinase 2 interacting protein-1 (CKIP-1) expression was induced in the murine MSC cell line C3H/10T1/2 by LPS.

Dual-stimuli-responsive TiO /DOX nanodrug system for lung cancer synergistic therapy.

Biological applications of nanosheets are rapidly increasing currently, which introduces new possibilities to improve the efficacy of cancer chemotherapy and radiotherapy. Herein, we designed and synthesized a novel nano-drug system, doxorubicin (DOX) loaded titanium peroxide (TiO ) nanosheets, toward the synergistic treatment of lung cancer. The precursor of TiO nanosheets with high specific surface area was synthesized by a modified hydrothermal process using the polymer P123 as a soft template to control the shape.

Defect-engineered TiO Hollow Spiny Nanocubes for Phenol Degradation under Visible Light Irradiation.

Herein, we mainly report a strategy for the facile synthesis of defect-engineered F-doped well-defined TiO2 hollow spiny nanocubes, constructed from NHTiOF as precursor. The topological transformation of NHTiOF mesocrystal is accompanied with fluorine anion releasing, which can be used as doping source to synthesize F-doped TiO. Our result shows that the introduction of oxygen vacancies (Vo's) and F dopant can be further achieved by a moderate photoreduction process.

[MicroRNA-20a Promotes Osteogenic Differentiation of C3H/10T1/2 Cells through Regulating CKIP-1 Expression].

Objective: To investigate the effect of microRNA-20a(MiR-20a) on osteogenic differentiation of mouse C3H/10T1/2 cells and its regulatory mechanism.

Methods: Osteogenic differentiation of C3H/10T1/2 cells were identified by ALP staining and qRT-PCR. MiR-20a mimics and CKIP-1 siRNA were transfected into C3H/10T1/2 cells respectively with lipo3000.

The stimulation of osteogenic differentiation of embryoid bodies from human induced pluripotent stem cells by akermanite bioceramics.

Induced pluripotent stem cells (iPSCs) have great potential as seed cells for tissue engineering applications. Previous studies have shown that iPSCs could be induced to differentiate into bone forming cells. However, in a tissue engineering approach, seeding cells in biomaterials is required, and the effect of biomaterials on cell growth and differentiation is critical for the success of the formation of engineered tissues.

Aging performances for resisting low-temperature of three dental Yttria-stabilized zirconia ceramic core materials.

Background: The low-temperature resistance aging performance of Yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) is the key effective factor that influences the long-term success rate of prosthesis. The objective of this study was to test and compare the aging performances for resisting low temperature of Lava Frame, Cercon Smart, and Upcera Yttria-stabilized zirconia core materials, via analyzing the micro and the crystal phases of the materials, and measure the three-point bending strength and the fracture toughness.

Methods: The three zirconia green bodies were prepared as 60 test samples for three-point bending strength and as 60 test samples for fracture toughness.

Posterolateral spinal fusion with nano-hydroxyapatite-collagen/PLA composite and autologous adipose-derived mesenchymal stem cells in a rabbit model.

Spinal fusion is routinely performed to treat low back pain caused by degeneration of intervertebral discs. An autologous bone graft derived from the iliac crest is the standard procedure used for spinal fusion. However, several shortcomings, including pseudarthrosis, pain and the need for blood transfusion are known to be associated with the procedure.

[Effect of right chewing-side preference on morphological temporomandibular joint changes].

Purpose: To explore the effect of right chewing-side preference on temporomandibular joint with cone-beam computed tomography (CBCT).

Methods: Fifty-two patients who were diagnosed as right chewing-side preference were selected and scanned by CBCT in the intercuspal position. The interested region was chosen to reconstruct and the images of bony structure of TMJ at axial view, coronal position, sagittal position, oblique position parallelled to the long axis of condyle and oblique position vertical to the long axis of condyle were evaluated.

Telocytes accompanying cardiomyocyte in primary culture: two- and three-dimensional culture environment.

Recently, the presence of telocytes was demonstrated in human and mammalian tissues and organs (digestive and extra-digestive organs, genitourinary organs, heart, placenta, lungs, pleura, striated muscle). Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium. By cultures of telocytes in two- and three-dimensional environment we aimed to study the typical morphological features as well as functionality of telocytes, which will provide important support to understand their in vivo roles.

Biocompatibility and Osteogenic Capacity of Periodontal Ligament Stem Cells on nHAC/PLA and HA/TCP Scaffolds.

This study investigated the effects of a newly-developed scaffold, nano-hydroxyapatite/collagen/poly(L-lactide) (nHAC/PLA), on the attachment, proliferation and osteogenic capability of dog periodontal ligament stem cells (PDLSCs) in vitro and in vivo. Hydroxyapatite/tricalcium phosphate (HA/TCP), a commonly used bone substitute, was used as a positive control. PDLSCs isolated from dog molar were incubated in an osteogenic medium to evaluate their osteogenic differentiation in vitro, and then seeded onto nHAC/PLA and HA/TCP scaffolds.

[Experiment on inducing human periodontal ligament stem cells into adipose cells].

Objective: To explore the capability of human periodontal ligament stem cells (PDLSCs) differentiating into adipose cells in vitro and to determine their changes in cell morphology, structure and function during differentiation.

Methods: PDLSCs isolated by magnetic-activated cell selection were treated continuously with adipogenic medium for 21 d. Then the cell morphology, ultrastructure, adipose specific markers of low density lipoprotein (LPL) and peroxisome proliferator activated receptor-gamma (PPAR-gamma) were analyzed by inverted contrast microscope, trans mission electron microscope (TEM), flow cytometry, immunofluorescence, RT-PCR and Western blot, respectively.

Gene-modified stem cells combined with rapid prototyping techniques: a novel strategy for periodontal regeneration.

Periodontal disease, a worldwide prevalent chronic disease in adults, is characterized by the destruction of the periodontal supporting tissue including the cementum, periodontal ligament and alveolar bone. The regeneration of damaged periodontal tissue is the main goal of periodontal treatment. Because conventional periodontal treatments remain insufficient to attain complete and reliable periodontal regeneration, periodontal tissue engineering has emerged as a prospective alternative method for improving the regenerative capacity of periodontal tissue.

Glimepiride induces proliferation and differentiation of rat osteoblasts via the PI3-kinase/Akt pathway.

Glimepiride is a third-generation sulfonylurea agent and is widely used in the treatment of type 2 diabetes mellitus. In addition to the stimulatory effects on pancreatic insulin secretion, glimepiride has also been reported to have extrapancreatic functions including activation of PI3 kinase (PI3K) and Akt in rat adipocytes and skeletal muscle. PI3-kinase and Akt are important signaling molecules in the regulation of proliferation and differentiation in various cells.