Genome and bioinformatic analysis of a HAdV-B14p1 virus isolated from a baby with pneumonia in Beijing, China.

The genome of HAdV-B14p1 strain BJ430, isolated from a six-month-old baby diagnosed with bronchial pneumonia at the Beijing Children's Hospital in December 2010, was sequenced, analyzed, and compared with reference adenovirus genome sequences archived in GenBank. This genome is 34,762 bp in length, remarkably presenting 99.9% identity with the genome from HAdV14p1 strain 303600, which was isolated in the USA (2006).

Complete genome sequence of human adenovirus type 7 associated with fatal infant pneumonia.

The Chinese human adenovirus 7 (HAdV7) 0901HZ/ShX/CHN/2009 was isolated from the hydrothorax fluid of an infant with fatal pneumonia in Shaanxi, China, in 2009. Comparison of the entire genome with the genomes of the other 10 strains of HAdV7 from GenBank revealed homologies of 89.9 to 99.

Human parainfluenza virus-associated respiratory tract infection among children and genetic analysis of HPIV-3 strains in Beijing, China.

The relevance of human parainfluenza viruses (HPIVs) to the epidemiology of acute respiratory infections (ARI) in China is unclear. From May 2008 to September 2010, 443 nasopharyngeal aspirates (NPAs) from hospitalized pediatric patients (age from 1 to 93 months) in Beijing were collected and screened for HPIVs and other common respiratory viruses by real-time RT-PCR. Sixty-two of 443 samples were positive for HPIVs with 4 positive for HPIV-2 and 58 positive for HPIV-3, indicating that HPIV-3 was the predominant virus present during the study period.

[Genetic characterization of coxsackievirus A16 isolated in Ningxia Hui Municipality in 2008].

Objective: To study the genetic characterization of coxsackievirus A16 (CVA16) strains isolated during an epidemic of hand, foot, and mouth disease (HFMD) in Ningxia Hui Municipality in 2008.

Methods: Clinical samples were collected from HFMD patients in Ningxia Hui Municipality and CVA16 strains were isolated by viral isolation methods. Reverse transcription-polymerase chain reactions (RT-PCR), specific for CVA16 were performed with these CVA16 strains.

Emergence and localized circulation of a vaccine-derived poliovirus in an isolated mountain community in Guangxi, China.

From March to May 2006, type 1 circulating vaccine-derived poliovirus (cVDPV) was isolated from one case patient with acute flaccid paralysis (AFP) and six unimmunized healthy contacts in isolated mountain villages in Guangxi, China. We conducted epidemiological investigations in the affected communities and nucleotide sequence analyses of the cVDPV isolates. The results of the investigations showed that the AFP patient, an unimmunized 10-year-old boy, and five laboratory-confirmed contacts lived in the same village; one contact lived in a neighboring village.

[Analysis on genetic characteristic of type I poliovirus in China in 2009].

Objective: To study the molecular characteristics of type 1 poliovirus isolated from the acute flaccid paralysis (AFP)surveillance system in China in 2009, to provide a scientific basis for maintaining polio-free status for China.

Method: Polymerase chain reaction (RT-PCR) method was used to amplify the VP1 code region of all the type I poliovirus, and the VP1 coding region of the isolated stains was sequenced and analyzed, the hot-spots and nuerovirulence determinant were analyzed. The phylogenetic tree was constructed based on VP1 region to analyze the evolutionary relationship between the strains.

[Evaluation of real time fluorescent quantitative reverse transcription polymerase chain reaction for detection of poliovirus].

Objective: Application of Real Time Fluorescent quantitative reverse transcription polymerase chani reaction (rRT-PCR)to poliovirus identification in Chinese Poliomyelitis Laboratory Network and evaluation of the assay.

Methods: According to Real-time RT-PCR recommended by WHO and developed by USA Centers for Disease Control and Prevention, 10 poliovirus isolates from laboratories of Chinese poliomyelitis network were tested for intratypic differentiation (ITD) and vaccine derived polioviruses (VDPVs) screening. The results of Real-time RT-PCR for 10 isolates were compared with those of VP, region sequencing.

Molecular evidence of persistent epidemic and evolution of subgenotype B1 coxsackievirus A16-associated hand, foot, and mouth disease in China.

The molecular epidemiology of CVA16 in China between 1999 and 2008 reflects a pattern of endemic cocirculation of clusters B1a and B1b within subgenotype B1 viruses. The annual evolution rate of CVA16 was estimated as approximately 0.91 x 10(-2) substitutions per synonymous nucleotide/year and is slightly lower than that of HEV71.