Bile acid biosynthesis during liver regeneration: enzyme activities of cholesterol 7alpha-hydroxylase and 3beta-hydroxy-delta5-C27-steroid dehydrogenase in rats.

The effects of two-thirds partial hepatectomy on bile acid metabolism have not been well established. This study investigated the changes in microsomal enzymes activities during liver regeneration. The cholesterol 7alpha-hydroxylase (7alpha-hydroxylase) and 3beta-hydroxy-delta5-C27-steroid dehydrogenase (3beta-dehydrogenase) activities in male Wistar rats were determined using high-performance liquid chromatography (HPLC).

Species difference in cholesterol 7alpha-hydroxylase expression of rabbit and rat liver microsomes after bile duct ligation.

Background: Bile duct ligation (BDL) produces a good animal model for investigation of the metabolic changes in obstructive jaundice. The aim of this study was to investigate the species difference in expression of cholesterol 7alpha-hydroxylase (7alpha-hydroxylase) in rabbits and rats after BDL.

Materials And Methods: Ten male New Zealand white rabbits weighing 2.

A comparative study of the conversion of 7-hydroxycholesterol in rabbit, guinea pig, rat, hamster, and chicken.

The metabolism of epimeric 7-hydroxycholesterol was studied in vitro. 7Alpha-hydroxycholesterol or 7beta-hydroxycholesterol were incubated with rabbit, guinea pig, rat, hamster, and chicken microsomal suspensions and then extracted and analyzed using high-performance liquid chromatography (HPLC). 7Alpha-hydroxy-4-cholesten-3-one was the main product from 7alpha-hydroxycholesterol in the rabbit, guinea pig, and rat.