Mechanistic insights into the inhibitory effect of theaflavins on virulence factors production in Streptococcus mutans.

Streptococcus mutans is the primary etiological agent associated with cariogenic process. The present study aimed to investigate the antibacterial and anti-virulence activities of theaflavins (TFs) to Streptococcus mutans UA159 as well as the underlying mechanisms. The results showed that TFs were capable of suppressing the acid production, cell adherence, water-insoluble exopolysaccharides production, and biofilm formation by S.

Tooth brushing using toothpaste containing theaflavins reduces the oral pathogenic bacteria in healthy adults.

Unlabelled: Theaflavins (TFs) are the main bioactive component in black tea. At present, little effort has been done to evaluate the influence of TFs when included in the toothpaste on the diversity of oral microbiota. In this study, eighty samples collected from the saliva and supragingival plaque of 20 healthy adults using toothpaste with the absence or presence of TFs for a period of 4 weeks were used for the oral microbiome analysis by 16S rRNA gene sequencing.

New insights into the antibacterial and quorum sensing inhibition mechanism of leaf extracts towards PAO1.

Unlabelled: This study aimed to investigate the anti-quorum sensing (QS) activity of leaf extracts (AALE) towards PAO1 as well as the underlying molecular mechanisms. Using a biosensor CV026, AALE were found to have anti-QS activity as AALE treatment significantly inhibited the violacein production of CV026 while produced little effect on the cell growth. Beyond that a higher dosage of AALE inhibited cell growth, sub-MIC of AALE significantly reduced the production of QS-regulated virulence factors (pyocyanin, elastase, and rhamnolipid), biofilm formation, and the swarming and swimming motility in PAO1 with a dosage-dependent manner.