Intracellular autolytic whole cell Salmonella vaccine prevents colonization of pathogenic Salmonella Typhimurium in chicken.

Salmonella enterica (SE) is a major foodborne bacterial pathogen in the United States, commonly found as the normal flora of various animals that is attributed to causing at least 1.2 million infections annually. Poultry plays a major role in disseminating SE through direct contact with live animals and consumption of contaminated products.

Eradication and Sensitization of Methicillin Resistant to Methicillin with Bioactive Extracts of Berry Pomace.

The therapeutic roles of phenolic blueberry () and blackberry () pomace (commercial byproduct) extracts (BPE) and their mechanism of actions were evaluated against methicillin resistant (MRSA). Five major phenolic acids of BPE, e.g.

Bioactive extracts from berry byproducts on the pathogenicity of Salmonella Typhimurium.

The aim of this study was to evaluate the phenotypic and genotypic properties of Salmonella enterica serovar Typhimurium (ST) in the presence of lethal and sublethal concentrations (SLC) of blackberry (Rubus fruticosus) and blueberry (Vaccinium corymbosum) pomace extracts. Antimicrobial susceptibility, physicochemical properties, motility, biofilm formation ability, virulence gene expression patterns, and the ability of ST to colonize in chick cecum were evaluated in the presence of these bioactive extracts. HPLC-MS analysis indicated that the phenolics in the berry pomace extracts consisted, but not limited to, flavan, flavanone, flavones, glucuronides, glucosides, quinolones, catechol, coumarin, phenols, luteolines, tannins, quercetin, chlorogenic acid, ellagic acid, gallic acid, and xanthoxic acid.

Cutting Edge: Expression of IRF8 in Gastric Epithelial Cells Confers Protective Innate Immunity against Helicobacter pylori Infection.

IFN regulatory factor 8 (IRF8) is expressed in many types of blood cells and plays critical roles in cellular differentiation and function. However, the role of IRF8 in nonhematopoietic systems remains poorly understood. In this study, we provide evidence that IRF8 is a transcriptional modulator of the gastric mucosa necessary for limiting Helicobacter pylori colonization.

Investigating the Role of Helicobacter pylori PriA Protein.

Background: In bacteria, PriA protein, a conserved DEXH-type DNA helicase, plays a central role in replication restart at stalled replication forks. Its unique DNA binding property allows it to recognize and stabilize stalled forks and the structures derived from them. PriA plays a very critical role in replication fork stabilization and DNA repair in E.

Prevalence of Helicobacter pylori infection and its relation with body mass index in a Chinese population.

Background: Helicobacter pylori infection is highly prevalent worldwide. The association between obesity and H. pylori infection is controversial in the literature.

Olfm4 deletion enhances defense against Staphylococcus aureus in chronic granulomatous disease.

Chronic granulomatous disease (CGD) patients have recurrent life-threatening bacterial and fungal infections. Olfactomedin 4 (OLFM4) is a neutrophil granule protein that negatively regulates host defense against bacterial infection. The goal of this study was to evaluate the impact of Olfm4 deletion on host defense against Staphylococcus aureus and Aspergillus fumigatus in a murine X-linked gp91phox-deficiency CGD model.

Genetic organization and conjugal plasmid DNA transfer of pHP69, a plasmid from a Korean isolate of Helicobacter pylori.

We isolated pHP69, a 9,153 bp plasmid from Helicobacter pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences.

Screening Helicobacter pylori genes induced during infection of mouse stomachs.

Aim: To investigate the effect of in vivo environment on gene expression in Helicobacter pylori (H. pylori) as it relates to its survival in the host.

Methods: In vivo expression technology (IVET) systems are used to identify microbial virulence genes.

A thin-layer liquid culture technique for the growth of Helicobacter pylori.

Background And Aims: Several attempts have been successful in liquid cultivation of Helicobaccter pylori. However, there is a need to improve the growth of H. pylori in liquid media in order to get affluent growth and a simple approach for examining bacterial properties.

Proteomic analysis of Helicobacter pylori cellular proteins fractionated by ammonium sulfate precipitation.

Among 1590 ORFs in the Helicobacter pylori genome, >250 have been identified as authentic genes by proteomic analysis. Low-abundance proteins need to be enriched to a minimal amount for MALDI-TOF analysis and salt precipitation has generally been used for protein enrichment. Here, a whole-cell extract of H.