Publications by authors named "Jung-Suk Kwon"
Previous studies show that porcine oocyte aging resulting from asynchronised IVM impairs embryo developmental competence. In the present study we investigated whether trichostatin A (TSA; an inhibitor of histone deacetylation) prolongs the maturation time and prevents the aging of oocytes. Porcine oocytes were cultured in medium containing increasing concentrations of TSA (300 nM) for 24, 44 or 64 h.
View Article and Find Full Text PDFAutophagy, an essential process for cellular maintenance, cell viability, and development, is the bulk degradation of proteins and organelles. This study investigated the expression levels of autophagy-related genes and the effect of 3-methyladenine (3-MA, an autophagy inhibitor) or rapamycin (an autophagy inducer) on maternal gene degradation and apoptosis in porcine parthenotes developing in vitro. LC3, which is essential for the formation of autophagosomes, was widely expressed in porcine parthenotes.
View Article and Find Full Text PDFArticle Synopsis
- - Mitochondria play a crucial role in regulating cell death (apoptosis) and the recycling of cellular components (autophagy), with reactive oxygen species (ROS) being significant triggers for these processes.
- - In a study using pig embryos (parthenotes), hydrogen peroxide (H2O2) concentration impacted developmental rates and mitochondrial health, with higher doses leading to abnormal mitochondrial shapes and increased signs of cell death and autophagy.
- - Analysis revealed that lower levels of H2O2 (100 µM) did not affect gene expression significantly, but higher levels (500 µM) decreased anti-apoptotic gene expression while increasing pro-apoptotic and autophagy-related genes, indicating that