Electrochemical detection of peroxynitrite using a biosensor based on a conducting polymer-manganese ion complex.

A peroxynitrite (ONOO(-)) biosensor has been developed through the preparation of a new manganese-[poly-2,5-di-(2-thienyl)-1H-pyrrole)-1-(p-benzoic acid)] (Mn-pDPB) complex. DPB monomer was first synthesized and polymerized for the purpose of providing a polymer backbone for complex formation with Mn(2+) ion. The Mn-pDPB complex was characterized via Magnetomotive Force (MMF) simulation, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry.

Amplification strategy based on gold nanoparticle-decorated carbon nanotubes for neomycin immunosensors.

A novel amperometric immunosensor with an enhanced sensitivity for the detection of neomycin (Neo) was prepared by covalently immobilizing a monoclonal Neo antibody onto a new conducting polymer, poly-[2,5-di-(2-thienyl)-1H-pyrrole-1-(p-benzoic acid)] (pDPB), as a sensor probe. The probe was used to detect Neo in a sandwich-type approach, where the secondary antibody was attached to gold nanoparticle-decorated multi-wall carbon nanotubes labeled with hydrazine (Hyd-MWCNT(AuNP)-Ab(2)). Hydrazine on the conjugate served as a catalyst for the reduction of hydrogen peroxide, and the catalytic current was monitored at -0.

Gold nanoparticles doped conducting polymer nanorod electrodes: ferrocene catalyzed aptamer-based thrombin immunosensor.

Au nanoparticles-doped conducting polymer nanorods electrodes (AuNPs/CPNEs) were prepared by coating Au nanorods (AuNRs) with a conducting polymer layer. The AuNRs were prepared through an electroless deposition method using the polycarbonate membrane (pore diameter, 50 nm, pore density, 6 x 10(8) pores/cm(2)) as a template. The AuNPs/CPNEs combining catalytic activity of ferrocene to ascorbic acid were used for the fabrication of an ultrasensitive aptamer sensor for thrombin detection.