Serum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism.

Extracellular vesicle (EV)-mediated transfer of long non-coding RNAs (lncRNAs) has been reported to regulate chemoresistance in various cancers. We herein investigate the therapeutic potential of bioinformatically identified HOTAIR transferred by serum-derived EVs (serum-EVs) in temozolomide (TMZ) resistance of glioblastoma (GBM) and the downstream mechanisms. EVs were isolated from the serum of GBM patients.

IGF2BP2 Induces U251 Glioblastoma Cell Chemoresistance by Inhibiting FOXO1-Mediated PID1 Expression Through Stabilizing lncRNA DANCR.

Glioma is the most common type of malignant tumor of the nervous system and is characterized by high mortality and poor outcome. This study aims to investigate the mechanism underlying IGF2 mRNA-binding protein 2 (IGF2BP2) and long noncoding RNA DANCR in etoposide resistance of glioblastoma (GBM) cells. Bioinformatics analysis identified the IGF2BP2-related regulators and DANCR target genes, which were subsequently evaluated by RNA pull-down and RIP assays.

Rapid and Accurate Detection of Lymph Node Metastases Enabled through Fluorescent Silicon Nanoparticles-Based Exosome Probes.

Sentinel lymph node (SLN) detection is of great significance for the prevention and treatment of cancer metastasis. Herein, we introduce silicon nanoparticles (SiNPs)-based exosome (SiNPs@EXO) probes for distinguishing normal and metastatic SLNs. Typically, SiNPs are suitable for stable and long-term tracking of exosomes, while cancer cell-driven exosomes with a tumor-homing effect allow targeting metastatic SLNs.

Targeted Noninvasive Treatment of Choroidal Neovascularization by Hybrid Cell-Membrane-Cloaked Biomimetic Nanoparticles.

Choroidal neovascularization (CNV) is the leading cause of vision loss in many blinding diseases, but current antiangiogenic therapies with invasively intravitreal injection suffer from poor patient compliance and a rate of devastating ocular complications. Here, we develop an alternative antiangiogenic agent based on hybrid cell-membrane-cloaked nanoparticles for noninvasively targeted treatment of CNV. The retinal endotheliocyte membrane coating provides as-fabricated nanoagents with homotypic targeting capability and binding ability to the vascular endothelial growth factor.

Hydrothermal Synthesis of Zinc-Doped Silica Nanospheres Simultaneously Featuring Stable Fluorescence and Long-Lived Room-Temperature Phosphorescence.

Fluorescence and phosphorescence are known as two kinds of fundamental optical signals, which have been used for myriad applications. To date, simultaneous activation of stable fluorescence and long-lived room-temperature phosphorescence (RTP) emission in the aqueous phase remains a big challenge. We prepare zinc-doped silica nanospheres (Zn@SiNSs) with fluorescence and RTP properties using a facile hydrothermal synthetic strategy.