Fluidized-bed agglomeration of native potato starch (NPS) with sugar binders [maltodextrin (MD) and lactose] produced larger particles with a more porous and diverse shape. Moreover, the agglomerated potato starch (APS) exhibited improved flowability and solubility. All powders displayed a B-type X-ray diffraction pattern.
View Article and Find Full Text PDFBiomacromolecules
November 2024
This study sought to explore the rheological and tribological properties of fucoidan-xanthan gum (XG) mixtures at different fucoidan concentrations. A conformational transition of XG from disordered to ordered forms was observed with an increasing fucoidan concentration, as determined by intrinsic viscosity measurements and Fourier transform infrared analysis. All mixtures exhibited non-Newtonian flow behavior with the yield stress.
View Article and Find Full Text PDFBecause the viscosity of thickened beverages prepared with thickeners gradually changes before consumption, achieving their desired viscosity is important for managing dysphagia. This study aimed to investigate the viscosity changes of thickened beverages (water, orange juice, and milk) prepared with xanthan gum (XG)- and starch-based commercial thickeners over time using the syringe flow test (SFT) and line-spread test (LST). The LST values of beverages stabilized more quickly (≤1.
View Article and Find Full Text PDFA goal of this study is to explore the difference in rheological properties of xanthan gum (XG)-based mixtures with gum arabic (GA) or GA-based emulsion (GAE). The stability of GAE was improved by thickening with XG. The intrinsic viscosity of all mixtures decreased as GA concentration increased, implying an XG conformational transition from the disordered to the ordered form.
View Article and Find Full Text PDFSulfated saccharides exhibit diverse physiological activities, but a lack of any convenient assay hinders their evaluation. Herein, an assay for the analysis of sulfated saccharides is described using H nuclear magnetic resonance (NMR) spectroscopy by employing ligands that can form ionic complexes with the sulfate groups. Based on the change in the chemical shift (Δδ) of the ligands by sulfated mono- to tetrasaccharide, imidazole was found to be a good ligand, showing the maximum Δδ; neutral saccharides do not show any change in the δ value.
View Article and Find Full Text PDFThe present study investigated immune stimulatory effects of Cladosiphon okamuranus-derived fucoidan to activate murine macrophage-like cell line RAW264, and the functional relationship with zymosan, a Saccharomyces cerevisiae-derived β-glucan. The production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in RAW264 cells were remarkably enhanced in the presence of 10 μg/mL fucoidan, and the stimulatory effects of fucoidan were maximally augmented in combinational treatment with 500 ng/mL zymosan, whereas any TLR ligands had no those effects. Confocal microscopic analyses suggested that fucoidan bound on plasma membrane, and it was estimated that some cell surface molecules acted as receptor for fucoidan because cytochalasin D, an inhibitor of phagocytosis, did not affect the immune enhancing activities, whereas methyl-β-cyclodextrin (MβCD), a general agent for disruption of lipid rafts, diminished that.
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